Association between Wisteria floribunda agglutinin-positive Mac-2 binding protein and the fibrosis stage of non-alcoholic fatty liver disease

Masanori Abe, Teruki Miyake, Atsushi Kuno, Yasuharu Imai, Yoshiyuki Sawai, Keisuke Hino, Yuichi Hara, Shuhei Hige, Michiie Sakamoto, Gotaro Yamada, Masayoshi Kage, Masaaki Korenaga, Yoichi Hiasa, Masashi Mizokami, Hisashi Narimatsu

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Background: Accurately evaluating liver fibrosis in patients with non-alcoholic fatty liver disease (NAFLD) is important for identifying those who may develop complications. The aims of this study were (1) to measure serum Wisteria floribunda agglutinin-positive Mac-2 binding protein (WFA<sup>+</sup>-M2BP) using the glycan sugar chain-based immunoassay and (2) to compare the results with clinical assessments of fibrosis. Methods: Serum WFA<sup>+</sup>-M2BP values were retrospectively evaluated in 289 patients with NAFLD who had undergone liver biopsy. Histological findings were evaluated by three blinded, experienced liver-specific pathologists. Results: For stages 0 (n = 35), 1 (n = 113), 2 (n = 49), 3 (n = 41), and 4 (n = 51) of liver fibrosis, the serum WFA<sup>+</sup>-M2BP cutoff indexes were 0.57, 0.70, 1.02, 1.57, and 2.96, respectively. Multivariate regression analysis showed that serum WFA<sup>+</sup>-M2BP values were associated with the stage of fibrosis (≥stage 2). The areas under the receiver operating characteristic curve (AUROC), sensitivity, and specificity of serum WFA<sup>+</sup>-M2BP were 0.876, 85.9, and 74.6 %, respectively, for severe fibrosis (≥stage 3) and were 0.879, 74.6, and 87.0 %, respectively, for cirrhosis. When compared with six non-invasive conventional markers, serum WFA<sup>+</sup>-M2BP had the greatest AUROC for diagnosing severe fibrosis and cirrhosis. Conclusions: Serum WFA<sup>+</sup>-M2BP values are useful for assessing the stage of liver fibrosis in patients with NAFLD.

Original languageEnglish
Pages (from-to)776-784
Number of pages9
JournalJournal of Gastroenterology
Volume50
Issue number7
DOIs
Publication statusPublished - 2014 Oct 18

Fingerprint

Carrier Proteins
Fibrosis
Serum
Liver Cirrhosis
ROC Curve
Liver
Immunoassay
Polysaccharides
wisteria lectin
Non-alcoholic Fatty Liver Disease
Multivariate Analysis
Biomarkers
Regression Analysis
Biopsy
Sensitivity and Specificity

Keywords

  • Cirrhosis
  • Fibrosis marker
  • Glycoprotein
  • Mac-2 binding protein
  • Non-alcoholic fatty liver disease

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Association between Wisteria floribunda agglutinin-positive Mac-2 binding protein and the fibrosis stage of non-alcoholic fatty liver disease. / Abe, Masanori; Miyake, Teruki; Kuno, Atsushi; Imai, Yasuharu; Sawai, Yoshiyuki; Hino, Keisuke; Hara, Yuichi; Hige, Shuhei; Sakamoto, Michiie; Yamada, Gotaro; Kage, Masayoshi; Korenaga, Masaaki; Hiasa, Yoichi; Mizokami, Masashi; Narimatsu, Hisashi.

In: Journal of Gastroenterology, Vol. 50, No. 7, 18.10.2014, p. 776-784.

Research output: Contribution to journalArticle

Abe, M, Miyake, T, Kuno, A, Imai, Y, Sawai, Y, Hino, K, Hara, Y, Hige, S, Sakamoto, M, Yamada, G, Kage, M, Korenaga, M, Hiasa, Y, Mizokami, M & Narimatsu, H 2014, 'Association between Wisteria floribunda agglutinin-positive Mac-2 binding protein and the fibrosis stage of non-alcoholic fatty liver disease', Journal of Gastroenterology, vol. 50, no. 7, pp. 776-784. https://doi.org/10.1007/s00535-014-1007-2
Abe, Masanori ; Miyake, Teruki ; Kuno, Atsushi ; Imai, Yasuharu ; Sawai, Yoshiyuki ; Hino, Keisuke ; Hara, Yuichi ; Hige, Shuhei ; Sakamoto, Michiie ; Yamada, Gotaro ; Kage, Masayoshi ; Korenaga, Masaaki ; Hiasa, Yoichi ; Mizokami, Masashi ; Narimatsu, Hisashi. / Association between Wisteria floribunda agglutinin-positive Mac-2 binding protein and the fibrosis stage of non-alcoholic fatty liver disease. In: Journal of Gastroenterology. 2014 ; Vol. 50, No. 7. pp. 776-784.
@article{6cc3187a3a8443f7971710621ff8c694,
title = "Association between Wisteria floribunda agglutinin-positive Mac-2 binding protein and the fibrosis stage of non-alcoholic fatty liver disease",
abstract = "Background: Accurately evaluating liver fibrosis in patients with non-alcoholic fatty liver disease (NAFLD) is important for identifying those who may develop complications. The aims of this study were (1) to measure serum Wisteria floribunda agglutinin-positive Mac-2 binding protein (WFA+-M2BP) using the glycan sugar chain-based immunoassay and (2) to compare the results with clinical assessments of fibrosis. Methods: Serum WFA+-M2BP values were retrospectively evaluated in 289 patients with NAFLD who had undergone liver biopsy. Histological findings were evaluated by three blinded, experienced liver-specific pathologists. Results: For stages 0 (n = 35), 1 (n = 113), 2 (n = 49), 3 (n = 41), and 4 (n = 51) of liver fibrosis, the serum WFA+-M2BP cutoff indexes were 0.57, 0.70, 1.02, 1.57, and 2.96, respectively. Multivariate regression analysis showed that serum WFA+-M2BP values were associated with the stage of fibrosis (≥stage 2). The areas under the receiver operating characteristic curve (AUROC), sensitivity, and specificity of serum WFA+-M2BP were 0.876, 85.9, and 74.6 {\%}, respectively, for severe fibrosis (≥stage 3) and were 0.879, 74.6, and 87.0 {\%}, respectively, for cirrhosis. When compared with six non-invasive conventional markers, serum WFA+-M2BP had the greatest AUROC for diagnosing severe fibrosis and cirrhosis. Conclusions: Serum WFA+-M2BP values are useful for assessing the stage of liver fibrosis in patients with NAFLD.",
keywords = "Cirrhosis, Fibrosis marker, Glycoprotein, Mac-2 binding protein, Non-alcoholic fatty liver disease",
author = "Masanori Abe and Teruki Miyake and Atsushi Kuno and Yasuharu Imai and Yoshiyuki Sawai and Keisuke Hino and Yuichi Hara and Shuhei Hige and Michiie Sakamoto and Gotaro Yamada and Masayoshi Kage and Masaaki Korenaga and Yoichi Hiasa and Masashi Mizokami and Hisashi Narimatsu",
year = "2014",
month = "10",
day = "18",
doi = "10.1007/s00535-014-1007-2",
language = "English",
volume = "50",
pages = "776--784",
journal = "Journal of Gastroenterology",
issn = "0944-1174",
publisher = "Springer Japan",
number = "7",

}

TY - JOUR

T1 - Association between Wisteria floribunda agglutinin-positive Mac-2 binding protein and the fibrosis stage of non-alcoholic fatty liver disease

AU - Abe, Masanori

AU - Miyake, Teruki

AU - Kuno, Atsushi

AU - Imai, Yasuharu

AU - Sawai, Yoshiyuki

AU - Hino, Keisuke

AU - Hara, Yuichi

AU - Hige, Shuhei

AU - Sakamoto, Michiie

AU - Yamada, Gotaro

AU - Kage, Masayoshi

AU - Korenaga, Masaaki

AU - Hiasa, Yoichi

AU - Mizokami, Masashi

AU - Narimatsu, Hisashi

PY - 2014/10/18

Y1 - 2014/10/18

N2 - Background: Accurately evaluating liver fibrosis in patients with non-alcoholic fatty liver disease (NAFLD) is important for identifying those who may develop complications. The aims of this study were (1) to measure serum Wisteria floribunda agglutinin-positive Mac-2 binding protein (WFA+-M2BP) using the glycan sugar chain-based immunoassay and (2) to compare the results with clinical assessments of fibrosis. Methods: Serum WFA+-M2BP values were retrospectively evaluated in 289 patients with NAFLD who had undergone liver biopsy. Histological findings were evaluated by three blinded, experienced liver-specific pathologists. Results: For stages 0 (n = 35), 1 (n = 113), 2 (n = 49), 3 (n = 41), and 4 (n = 51) of liver fibrosis, the serum WFA+-M2BP cutoff indexes were 0.57, 0.70, 1.02, 1.57, and 2.96, respectively. Multivariate regression analysis showed that serum WFA+-M2BP values were associated with the stage of fibrosis (≥stage 2). The areas under the receiver operating characteristic curve (AUROC), sensitivity, and specificity of serum WFA+-M2BP were 0.876, 85.9, and 74.6 %, respectively, for severe fibrosis (≥stage 3) and were 0.879, 74.6, and 87.0 %, respectively, for cirrhosis. When compared with six non-invasive conventional markers, serum WFA+-M2BP had the greatest AUROC for diagnosing severe fibrosis and cirrhosis. Conclusions: Serum WFA+-M2BP values are useful for assessing the stage of liver fibrosis in patients with NAFLD.

AB - Background: Accurately evaluating liver fibrosis in patients with non-alcoholic fatty liver disease (NAFLD) is important for identifying those who may develop complications. The aims of this study were (1) to measure serum Wisteria floribunda agglutinin-positive Mac-2 binding protein (WFA+-M2BP) using the glycan sugar chain-based immunoassay and (2) to compare the results with clinical assessments of fibrosis. Methods: Serum WFA+-M2BP values were retrospectively evaluated in 289 patients with NAFLD who had undergone liver biopsy. Histological findings were evaluated by three blinded, experienced liver-specific pathologists. Results: For stages 0 (n = 35), 1 (n = 113), 2 (n = 49), 3 (n = 41), and 4 (n = 51) of liver fibrosis, the serum WFA+-M2BP cutoff indexes were 0.57, 0.70, 1.02, 1.57, and 2.96, respectively. Multivariate regression analysis showed that serum WFA+-M2BP values were associated with the stage of fibrosis (≥stage 2). The areas under the receiver operating characteristic curve (AUROC), sensitivity, and specificity of serum WFA+-M2BP were 0.876, 85.9, and 74.6 %, respectively, for severe fibrosis (≥stage 3) and were 0.879, 74.6, and 87.0 %, respectively, for cirrhosis. When compared with six non-invasive conventional markers, serum WFA+-M2BP had the greatest AUROC for diagnosing severe fibrosis and cirrhosis. Conclusions: Serum WFA+-M2BP values are useful for assessing the stage of liver fibrosis in patients with NAFLD.

KW - Cirrhosis

KW - Fibrosis marker

KW - Glycoprotein

KW - Mac-2 binding protein

KW - Non-alcoholic fatty liver disease

UR - http://www.scopus.com/inward/record.url?scp=84937252875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937252875&partnerID=8YFLogxK

U2 - 10.1007/s00535-014-1007-2

DO - 10.1007/s00535-014-1007-2

M3 - Article

C2 - 25326152

AN - SCOPUS:84937252875

VL - 50

SP - 776

EP - 784

JO - Journal of Gastroenterology

JF - Journal of Gastroenterology

SN - 0944-1174

IS - 7

ER -