Association of autophagy status with amount of Fusobacterium nucleatum in colorectal cancer

Koichiro Haruki, Keisuke Kosumi, Tsuyoshi Hamada, Tyler S. Twombly, Juha P. Väyrynen, Sun A. Kim, Yohei Masugi, Zhi Rong Qian, Kosuke Mima, Yoshifumi Baba, Annacarolina da Silva, Jennifer Borowsky, Kota Arima, Kenji Fujiyoshi, Mai Chan Lau, Peilong Li, Chunguang Guo, Yang Chen, Mingyang Song, Jonathan A. NowakReiko Nishihara, Katsuhiko Yanaga, Xuehong Zhang, Kana Wu, Susan Bullman, Wendy S. Garrett, Curtis Huttenhower, Jeffrey A. Meyerhardt, Marios Giannakis, Andrew T. Chan, Charles S. Fuchs, Shuji Ogino

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fusobacterium nucleatum (F. nucleatum), which has been associated with colorectal carcinogenesis, can impair anti-tumour immunity, and actively invade colon epithelial cells. Considering the critical role of autophagy in host defence against microorganisms, we hypothesised that autophagic activity of tumour cells might influence the amount of F. nucleatum in colorectal cancer tissue. Using 724 rectal and colon cancer cases within the Nurses' Health Study and the Health Professionals Follow-up Study, we evaluated autophagic activity of tumour cells by immunohistochemical analyses of BECN1 (beclin 1), MAP1LC3 (LC3), and SQSTM1 (p62) expression. We measured the amount of F. nucleatum DNA in tumour tissue by quantitative polymerase chain reaction (PCR). We conducted multivariable ordinal logistic regression analyses to examine the association of tumour BECN1, MAP1LC3, and SQSTM1 expression with the amount of F. nucleatum, adjusting for potential confounders, including microsatellite instability status; CpG island methylator phenotype; long-interspersed nucleotide element-1 methylation; and KRAS, BRAF, and PIK3CA mutations. Compared with BECN1-low cases, BECN1-intermediate and BECN1-high cases were associated with lower amounts of F. nucleatum with odds ratios (for a unit increase in three ordinal categories of the amount of F. nucleatum) of 0.54 (95% confidence interval, 0.29–0.99) and 0.31 (95% confidence interval, 0.16–0.60), respectively (Ptrend < 0.001 across ordinal BECN1 categories). Tumour MAP1LC3 and SQSTM1 levels were not significantly associated with the amount of F. nucleatum (Ptrend > 0.06). Tumour BECN1, MAP1LC3, and SQSTM1 levels were not significantly associated with patient survival (Ptrend > 0.10). In conclusion, tumour BECN1 expression is inversely associated with the amount of F. nucleatum in colorectal cancer tissue, suggesting a possible role of autophagy in the elimination of invasive microorganisms.

Original languageEnglish
Pages (from-to)397-408
Number of pages12
JournalJournal of Pathology
Volume250
Issue number4
DOIs
Publication statusPublished - 2020 Apr 1
Externally publishedYes

Keywords

  • Colorectal neoplasms
  • Immunology
  • Microbiology
  • Microbiome
  • Molecular pathological epidemiology
  • Tumour microenvironment

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Association of autophagy status with amount of Fusobacterium nucleatum in colorectal cancer'. Together they form a unique fingerprint.

  • Cite this

    Haruki, K., Kosumi, K., Hamada, T., Twombly, T. S., Väyrynen, J. P., Kim, S. A., Masugi, Y., Qian, Z. R., Mima, K., Baba, Y., da Silva, A., Borowsky, J., Arima, K., Fujiyoshi, K., Lau, M. C., Li, P., Guo, C., Chen, Y., Song, M., ... Ogino, S. (2020). Association of autophagy status with amount of Fusobacterium nucleatum in colorectal cancer. Journal of Pathology, 250(4), 397-408. https://doi.org/10.1002/path.5381