Association of the myosin-binding subunit of myosin phosphatase and moesin: Dual regulation of moesin phosphorylation by Rho-associated kinase and myosin phosphatase

Yuko Fukata, Kazushi Kimura, Noriko Oshiro, Hideyuki Saya, Yoshiharu Matsuura, Kozo Kaibuchi

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

The small GTPase Rho is believed to regulate the actin cytoskeleton and cell adhesion through its specific targets. We previously identified the Rho targets: protein kinase N, Rho-associated kinase (Rho-kinase), and the myosin-binding subunit (MBS) of myosin phosphatase. We found that in MDCK epithelial cells, MBS accumulated at the tetradecanoylphorbol-13-acetate (TPA)-induced membrane ruffling area, where moesin, a member of the ERM (ezrin, radixin, and moesin) family, was localized. Neither membrane ruffling nor an accumulation of moesin and MBS at the free-end plasma membrane was induced when MDCK cells were stimulated with TPA after the microinjection of C3, which ADP-ribosylates and inactivates Rho. MBS was colocalized with moesin at the cell-cell contact sites in MDCK cells. We also found that moesin was coimmunoprecipitated with MBS from MDCK cells. Recombinant MBS interacted with the amino-terminal domains of moesin and ezrin. Myosin phosphatase composed of the catalytic subunit and MBS showed phosphatase activity toward moesin, which was phosphorylated by Rho-kinase. The phosphatase activity was inhibited when MBS was phosphorylated by Rho- kinase. These results suggest that MBS is recruited with moesin to the plasma membrane and that myosin phosphatase and Rho-kinase regulate the phosphorylation state of moesin downstream of Rho.

Original languageEnglish
Pages (from-to)409-418
Number of pages10
JournalJournal of Cell Biology
Volume141
Issue number2
DOIs
Publication statusPublished - 1998 Apr 20
Externally publishedYes

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Myosin-Light-Chain Phosphatase
rho-Associated Kinases
Myosins
Phosphorylation
Madin Darby Canine Kidney Cells
Tetradecanoylphorbol Acetate
Phosphoric Monoester Hydrolases
Cell Membrane
moesin
Membranes
Monomeric GTP-Binding Proteins
Microinjections
Actin Cytoskeleton
Cell Adhesion
Adenosine Diphosphate
Catalytic Domain
Epithelial Cells

ASJC Scopus subject areas

  • Cell Biology

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Association of the myosin-binding subunit of myosin phosphatase and moesin : Dual regulation of moesin phosphorylation by Rho-associated kinase and myosin phosphatase. / Fukata, Yuko; Kimura, Kazushi; Oshiro, Noriko; Saya, Hideyuki; Matsuura, Yoshiharu; Kaibuchi, Kozo.

In: Journal of Cell Biology, Vol. 141, No. 2, 20.04.1998, p. 409-418.

Research output: Contribution to journalArticle

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