Astrocyte calcium signalling orchestrates neuronal synchronization in organotypic hippocampal slices

Takuya Sasaki, Tomoe Ishikawa, Reimi Abe, Ryota Nakayama, Akiko Asada, Norio Matsuki, Yuji Ikegaya

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Astrocytes are thought to detect neuronal activity in the form of intracellular calcium elevations; thereby, astrocytes can regulate neuronal excitability and synaptic transmission. Little is known, however, about how the astrocyte calcium signal regulates the activity of neuronal populations. In this study, we addressed this issue using functional multineuron calcium imaging in hippocampal slice cultures. Under normal conditions, CA3 neuronal networks exhibited temporally correlated activity patterns, occasionally generating large synchronization among a subset of cells. The synchronized neuronal activity was correlated with astrocyte calcium events. Calcium buffering by an intracellular injection of a calcium chelator into multiple astrocytes reduced the synaptic strength of unitary transmission between pairs of surrounding pyramidal cells and caused desynchronization of the neuronal networks. Uncaging the calcium in the astrocytes increased the frequency of neuronal synchronization. These data suggest an essential role of the astrocyte calcium signal in the maintenance of basal neuronal function at the circuit level.

Original languageEnglish
Pages (from-to)2771-2783
Number of pages13
JournalJournal of Physiology
Volume592
Issue number13
DOIs
Publication statusPublished - 2014 Jul 1
Externally publishedYes

ASJC Scopus subject areas

  • Physiology

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