Abstract
To survey the emergence and onset of differentiation of the astrocytic lineage in the developing mouse cerebral wall, the promoter activity of a 2.5 kb 5'-flanking region of glial fibrillary acidic protein (GFAP) was measured in individual developing brain cells using a retrovirus-mediated gene transfer system. We identified precursors for astrocytes in primary culture of embryonic mouse cerebral wall cells by detection of GFAP promoter activity, which was detected approximately 3 days prior to the appearance of GFAP immunoreactivity. Since retroviruses only integrate into the chromosomes of actively proliferating cells, cells detected by this method should have been mitotically active at the time of retroviral infection on day 15 postfertilization (E15). Furthermore, we observed that cells activating GFAP promoter were located near the ventricular surface of cultured cerebral wall slices as a cluster of spherical cells. These results demonstrate that precursor cells for astrocytes exist within the germinative zone of developing cerebral wall, and that these cells are mitotically active on day E15, which is a late stage of neuronal production period in the mouse cerebral wall. The morphology, location and mitotic activity of these cells suggest that they are unlikely to be cells that have been transformed from radial glial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 210-218 |
| Number of pages | 9 |
| Journal | Developmental Neuroscience |
| Volume | 19 |
| Issue number | 2 |
| Publication status | Published - 1997 Mar |
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Keywords
- Astrocyte
- Cerebral wall slice culture
- Glial fibrillary acidic protein
- Primary culture
- Retroviral vector
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Astrocytic lineage analysis by detection of GFAP promoter activity in vitro. / Morita, Noriyuki; Nakahira, Kensuke; Baba, Hiroko; Akita, Hiromi; Kumada, Tatsuro; Ogawa, Masaharu; Nakajima, Kazunori; Kawata, Mitsuhiro; Mikoshiba, Katsuhiko; Ikenaka, Kazuhiro.
In: Developmental Neuroscience, Vol. 19, No. 2, 03.1997, p. 210-218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Astrocytic lineage analysis by detection of GFAP promoter activity in vitro
AU - Morita, Noriyuki
AU - Nakahira, Kensuke
AU - Baba, Hiroko
AU - Akita, Hiromi
AU - Kumada, Tatsuro
AU - Ogawa, Masaharu
AU - Nakajima, Kazunori
AU - Kawata, Mitsuhiro
AU - Mikoshiba, Katsuhiko
AU - Ikenaka, Kazuhiro
PY - 1997/3
Y1 - 1997/3
N2 - To survey the emergence and onset of differentiation of the astrocytic lineage in the developing mouse cerebral wall, the promoter activity of a 2.5 kb 5'-flanking region of glial fibrillary acidic protein (GFAP) was measured in individual developing brain cells using a retrovirus-mediated gene transfer system. We identified precursors for astrocytes in primary culture of embryonic mouse cerebral wall cells by detection of GFAP promoter activity, which was detected approximately 3 days prior to the appearance of GFAP immunoreactivity. Since retroviruses only integrate into the chromosomes of actively proliferating cells, cells detected by this method should have been mitotically active at the time of retroviral infection on day 15 postfertilization (E15). Furthermore, we observed that cells activating GFAP promoter were located near the ventricular surface of cultured cerebral wall slices as a cluster of spherical cells. These results demonstrate that precursor cells for astrocytes exist within the germinative zone of developing cerebral wall, and that these cells are mitotically active on day E15, which is a late stage of neuronal production period in the mouse cerebral wall. The morphology, location and mitotic activity of these cells suggest that they are unlikely to be cells that have been transformed from radial glial cells.
AB - To survey the emergence and onset of differentiation of the astrocytic lineage in the developing mouse cerebral wall, the promoter activity of a 2.5 kb 5'-flanking region of glial fibrillary acidic protein (GFAP) was measured in individual developing brain cells using a retrovirus-mediated gene transfer system. We identified precursors for astrocytes in primary culture of embryonic mouse cerebral wall cells by detection of GFAP promoter activity, which was detected approximately 3 days prior to the appearance of GFAP immunoreactivity. Since retroviruses only integrate into the chromosomes of actively proliferating cells, cells detected by this method should have been mitotically active at the time of retroviral infection on day 15 postfertilization (E15). Furthermore, we observed that cells activating GFAP promoter were located near the ventricular surface of cultured cerebral wall slices as a cluster of spherical cells. These results demonstrate that precursor cells for astrocytes exist within the germinative zone of developing cerebral wall, and that these cells are mitotically active on day E15, which is a late stage of neuronal production period in the mouse cerebral wall. The morphology, location and mitotic activity of these cells suggest that they are unlikely to be cells that have been transformed from radial glial cells.
KW - Astrocyte
KW - Cerebral wall slice culture
KW - Glial fibrillary acidic protein
KW - Primary culture
KW - Retroviral vector
UR - http://www.scopus.com/inward/record.url?scp=0031046567&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031046567&partnerID=8YFLogxK
M3 - Article
C2 - 9097037
AN - SCOPUS:0031046567
VL - 19
SP - 210
EP - 218
JO - Developmental Neuroscience
JF - Developmental Neuroscience
SN - 0378-5866
IS - 2
ER -