Astrocytic lineage analysis by detection of gfap promoter activity in vitro

Noriyuki Morita, Kensuke Nakahira, Hiroko Baba, Hiromi Akira, Tatsuro Kumada, Masahara Ogawa, Kazunori Nakajima, Mitsuhiro Kawata, Katsnhiko Mikoshiba, Kazuhiro Lkenaka

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10 Citations (Scopus)

Abstract

To survey the emergence and onset of differentiation of the astrocytic lineage in the developing mouse cerebral wall, the promoter activity of a 2.5 kb 5''-flanking region of glial fibrillary acidic protein (GFAP) was measured in individual developing brain cells using a retrovirus-mediated gene transfer system. We identified precursors for astrocytes in primary culture of embryonic mouse cerebral wall cells by detection of GFAP promoter activity, which was detected approximately 3 days prior to the appearance of GFAP immunoreactivity. Since retroviruses only integrate into the chromosomes of actively proliferating cells, cells detected by this method should have been mitotically active at the time of retroviral infection on day 15 postfertilization (E15). Furthermore, we observed that cells activating GFAP promoter were located near the ventricular surface of cultured cerebral wall slices as a cluster of spherical cells. These results demonstrate that precursor cells for astrocytes exist within the germinative zone of developing cerebral wall, and that these cells are mitotically active on day E15, which is a late stage of neuronal production period in the mouse cerebral wall. The morphology, location and mitotic activity of these cells suggest that they are unlikely to be cells that have been transformed from radial glial cells.

Original languageEnglish
Pages (from-to)210-218
Number of pages9
JournalDermatology
Volume19
Issue number2
DOIs
Publication statusPublished - 1997 Jan

Keywords

  • Astrocyte
  • Cerebral wall slice culture
  • Glial fibrillary acidic protein
  • Primary culture
  • Retroviral vector

ASJC Scopus subject areas

  • Dermatology

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    Morita, N., Nakahira, K., Baba, H., Akira, H., Kumada, T., Ogawa, M., Nakajima, K., Kawata, M., Mikoshiba, K., & Lkenaka, K. (1997). Astrocytic lineage analysis by detection of gfap promoter activity in vitro. Dermatology, 19(2), 210-218. https://doi.org/10.1159/000111208