Rhodococcus rhodochrous ATCC 21197 hydrolyzed prochiral butylmethylmalononitrile to afford the corresponding amide-carboxylic acid with high enantiomeric excess. The reaction proceeds via the hydration of the starting dinitrile by a nitrile hydratase and the subsequent enantioselective hydrolysis of the intermediate diamide by an amidase.
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry
- Inorganic Chemistry