Asymmetric hydrolysis of a disubstituted malononitrile by the aid of a microorganism

Masahiro Yokoyama, Takeshi Sugai, Hiromichi Ohta

Research output: Contribution to journalArticle

46 Citations (Scopus)


Rhodococcus rhodochrous ATCC 21197 hydrolyzed prochiral butylmethylmalononitrile to afford the corresponding amide-carboxylic acid with high enantiomeric excess. The reaction proceeds via the hydration of the starting dinitrile by a nitrile hydratase and the subsequent enantioselective hydrolysis of the intermediate diamide by an amidase.

Original languageEnglish
Pages (from-to)1081-1084
Number of pages4
JournalTetrahedron: Asymmetry
Issue number6
Publication statusPublished - 1993 Jun


ASJC Scopus subject areas

  • Catalysis
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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