Asymmetry in the mitotic spindle induced by the attachment to the cell surface during maturation in the starfish oocyte

S. K. Satoh, M. T. Oka, Y. Hamaguchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In order to study the dynamic behavior of the mitotic apparatus leading to unequal cleavage, we investigated the distribution of mitotic microtubules (MTs) during maturation division of starfish oocytes. When the mitotic apparatus attached to the cell surface at metaphase, in both the first and second meiotic division, it is revealed, by immunofluorescence, that the MT distribution in the spindle, as well as in the aster, became asymmetric. MTs in the peripheral half spindle increased in number compared with those in the inner half spindle. Furthermore, these results were confirmed in the living cell by polarization microscopy; shortly after the attachment, the birefringence retardation of the peripheral half spindle became greater than that of the inner one, and the difference increased with time during anaphase. By inhibiting the attachment of the mitotic apparatus by means of centrifugation, the MT distribution maintained a symmetrical pattern through mitosis. These results suggest that the attachment of the mitotic apparatus to the cell surface induces the asymmetrical distribution of MTs not only in the aster but also in the spindle. Such a rich distribution of MTs in the peripheral half spindle appears to ensure chromosome exclusion into the polar body by anchoring them firmly to the cell surface of the animal pole.

Original languageEnglish
Pages (from-to)557-565
Number of pages9
JournalDevelopment Growth and Differentiation
Volume36
Issue number6
Publication statusPublished - 1994

Fingerprint

Starfish
Spindle Apparatus
Microtubules
Oocytes
Polarization Microscopy
Polar Bodies
Birefringence
Anaphase
Metaphase
Centrifugation
Mitosis
Fluorescent Antibody Technique
Chromosomes

Keywords

  • immunofluorescence
  • maturation
  • meiosis
  • microtubule
  • spindle

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Anatomy

Cite this

Asymmetry in the mitotic spindle induced by the attachment to the cell surface during maturation in the starfish oocyte. / Satoh, S. K.; Oka, M. T.; Hamaguchi, Y.

In: Development Growth and Differentiation, Vol. 36, No. 6, 1994, p. 557-565.

Research output: Contribution to journalArticle

@article{b56ff60ea34f4d7580eb671953d77025,
title = "Asymmetry in the mitotic spindle induced by the attachment to the cell surface during maturation in the starfish oocyte",
abstract = "In order to study the dynamic behavior of the mitotic apparatus leading to unequal cleavage, we investigated the distribution of mitotic microtubules (MTs) during maturation division of starfish oocytes. When the mitotic apparatus attached to the cell surface at metaphase, in both the first and second meiotic division, it is revealed, by immunofluorescence, that the MT distribution in the spindle, as well as in the aster, became asymmetric. MTs in the peripheral half spindle increased in number compared with those in the inner half spindle. Furthermore, these results were confirmed in the living cell by polarization microscopy; shortly after the attachment, the birefringence retardation of the peripheral half spindle became greater than that of the inner one, and the difference increased with time during anaphase. By inhibiting the attachment of the mitotic apparatus by means of centrifugation, the MT distribution maintained a symmetrical pattern through mitosis. These results suggest that the attachment of the mitotic apparatus to the cell surface induces the asymmetrical distribution of MTs not only in the aster but also in the spindle. Such a rich distribution of MTs in the peripheral half spindle appears to ensure chromosome exclusion into the polar body by anchoring them firmly to the cell surface of the animal pole.",
keywords = "immunofluorescence, maturation, meiosis, microtubule, spindle",
author = "Satoh, {S. K.} and Oka, {M. T.} and Y. Hamaguchi",
year = "1994",
language = "English",
volume = "36",
pages = "557--565",
journal = "Development, growth & differentiation",
issn = "0012-1592",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Asymmetry in the mitotic spindle induced by the attachment to the cell surface during maturation in the starfish oocyte

AU - Satoh, S. K.

AU - Oka, M. T.

AU - Hamaguchi, Y.

PY - 1994

Y1 - 1994

N2 - In order to study the dynamic behavior of the mitotic apparatus leading to unequal cleavage, we investigated the distribution of mitotic microtubules (MTs) during maturation division of starfish oocytes. When the mitotic apparatus attached to the cell surface at metaphase, in both the first and second meiotic division, it is revealed, by immunofluorescence, that the MT distribution in the spindle, as well as in the aster, became asymmetric. MTs in the peripheral half spindle increased in number compared with those in the inner half spindle. Furthermore, these results were confirmed in the living cell by polarization microscopy; shortly after the attachment, the birefringence retardation of the peripheral half spindle became greater than that of the inner one, and the difference increased with time during anaphase. By inhibiting the attachment of the mitotic apparatus by means of centrifugation, the MT distribution maintained a symmetrical pattern through mitosis. These results suggest that the attachment of the mitotic apparatus to the cell surface induces the asymmetrical distribution of MTs not only in the aster but also in the spindle. Such a rich distribution of MTs in the peripheral half spindle appears to ensure chromosome exclusion into the polar body by anchoring them firmly to the cell surface of the animal pole.

AB - In order to study the dynamic behavior of the mitotic apparatus leading to unequal cleavage, we investigated the distribution of mitotic microtubules (MTs) during maturation division of starfish oocytes. When the mitotic apparatus attached to the cell surface at metaphase, in both the first and second meiotic division, it is revealed, by immunofluorescence, that the MT distribution in the spindle, as well as in the aster, became asymmetric. MTs in the peripheral half spindle increased in number compared with those in the inner half spindle. Furthermore, these results were confirmed in the living cell by polarization microscopy; shortly after the attachment, the birefringence retardation of the peripheral half spindle became greater than that of the inner one, and the difference increased with time during anaphase. By inhibiting the attachment of the mitotic apparatus by means of centrifugation, the MT distribution maintained a symmetrical pattern through mitosis. These results suggest that the attachment of the mitotic apparatus to the cell surface induces the asymmetrical distribution of MTs not only in the aster but also in the spindle. Such a rich distribution of MTs in the peripheral half spindle appears to ensure chromosome exclusion into the polar body by anchoring them firmly to the cell surface of the animal pole.

KW - immunofluorescence

KW - maturation

KW - meiosis

KW - microtubule

KW - spindle

UR - http://www.scopus.com/inward/record.url?scp=0028583108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028583108&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0028583108

VL - 36

SP - 557

EP - 565

JO - Development, growth & differentiation

JF - Development, growth & differentiation

SN - 0012-1592

IS - 6

ER -