Atomic force microscopy study on the unfolding of globular proteins in the Langmuir films

Taiji Furuno

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Protein Langmuir films have been studied by atomic force microscopy (AFM). Ten standard proteins were spread at the air/water interface. Among them, seven formed stable interfacial films and were transferred onto silicon wafer using the Langmuir-Schaefer method to be studied by AFM. The concentration of the protein was in a concentrated regime as > 1.5 mg/m2, and the granules of the protein were contained in most transferred films. The dynamic structural changes of carbonic anhydrase (CA) at the air/water interface were examined as the decrease in the density of protein granules in relation to the emergence of the surface pressure. Merging of intact CA molecules into the unfolded CA film was suggested. It was observed that spreading a high concentration of protein solution delivered a high density of protein granules in the Langmuir film, although ordered arrays were never found. These results support the historically proposed structure for the unfolding of proteins in the concentrated protein Langmuir film, the mosaic structure, instead of the duplex conformation. Furthermore, the present study demonstrated at the molecular resolution that the Langmuir-Schaefer technique is a facile method to immobilize proteins with granular configuration onto solid surfaces in water.

Original languageEnglish
Pages (from-to)170-179
Number of pages10
JournalThin Solid Films
Volume552
DOIs
Publication statusPublished - 2014 Feb 3

Fingerprint

Langmuir Blodgett films
Atomic force microscopy
atomic force microscopy
proteins
Proteins
Carbonic anhydrase
carbonic anhydrase
Carbonic Anhydrases
Water
water
dynamic structural analysis
air
Structural dynamics
Air
Silicon wafers
Merging
solid surfaces
Conformations
wafers
Molecules

Keywords

  • Dense packing
  • Langmuir-Schaefer method
  • Mosaic structure
  • Protein Langmuir film
  • Unfolding of protein

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry
  • Metals and Alloys
  • Surfaces, Coatings and Films
  • Surfaces and Interfaces

Cite this

Atomic force microscopy study on the unfolding of globular proteins in the Langmuir films. / Furuno, Taiji.

In: Thin Solid Films, Vol. 552, 03.02.2014, p. 170-179.

Research output: Contribution to journalArticle

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