Abstract
Purpose: The current study was done to determine whether atorvastatin, the HMGCoA (3-hydroxy-3-methylglutaryl CoA) reductase inhibitor, could decrease renal transforming growth factor-β (TGF-β) levels in unilateral ureteral obstruction (UUO) and concomitantly affect renal tissue damage in UUO. Materials and Methods: Atorvastatin (20 mg/kg) was administered to rats 1 day prior to UUO and every day thereafter. Kidneys were harvested at day 14 after UUO. Tissue TGF-β was measured by bioassay using mink lung epithelial cells. Renal tubular proliferation and apoptosis were detected by immunostaining proliferating cell nuclear antigen and polyclonal antisingle strand DNA antibody, respectively. Fibrosis was assessed by measuring collagen deposition with trichrome stained slides. Interstitial leukocyte was detected by immunostaining CD45. Results: TGF-β bioassay showed that the obstructed kidney in the control group contained significantly higher TGF-β than the unobstructed kidney in the control group (mean ± SD 79.1 ± 48.5 vs 28.7 ± 13.7 pg/mg tissue) and atorvastatin significantly decrease tissue TGF-β in the obstructed kidney (53.4 ± 37.0 pg/mg tissue). Immunostaining polyclonal antisingle strand DNA antibody demonstrated that the obstructed kidney in the control group has significantly more tubular apoptosis than the unobstructed counterpart (4.8 ± 2.8 vs 2.1 ± 1.2 nuclei per high power field) and atorvastatin significantly decreased renal tubular apoptosis in the obstructed kidney (1.1 ± 0.7 nuclei per high power field). In addition, immunostaining proliferating cell nuclear antigen showed that the obstructed kidney in the atorvastatin group had significantly more renal tubular proliferation than the obstructed kidney in the control group (48.7 ± 20.8 vs 17.3 ± 10.6 per high power field). Control obstructed kidney showed significantly more fibrosis, which was also blunted by atorvastatin. Conclusions: Atorvastatin significantly decreases tissue TGF-β, resulting in a decrease in tubular damage and interstitial fibrosis. This suggests that atorvastatin is a promising agent for preventing renal tubular damage in UUO.
| Original language | English |
|---|---|
| Pages (from-to) | 2456-2459 |
| Number of pages | 4 |
| Journal | Journal of Urology |
| Volume | 172 |
| Issue number | 6 I |
| DOIs | |
| Publication status | Published - 2004 Dec |
| Externally published | Yes |
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Keywords
- Apoptosis
- Atorvastatin
- Kidney
- Transforming growth factor beta
- Ureteral obstruction
ASJC Scopus subject areas
- Urology
Cite this
Atorvastatin ameliorates renal tissue damage in unilateral ureteral obstruction. / Mizuguchi, Yasunori; Miyajima, Akira; Kosaka, Takeo; Asano, Takako; Asano, Tomohiko; Hayakawa, Masamichi.
In: Journal of Urology, Vol. 172, No. 6 I, 12.2004, p. 2456-2459.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Atorvastatin ameliorates renal tissue damage in unilateral ureteral obstruction
AU - Mizuguchi, Yasunori
AU - Miyajima, Akira
AU - Kosaka, Takeo
AU - Asano, Takako
AU - Asano, Tomohiko
AU - Hayakawa, Masamichi
PY - 2004/12
Y1 - 2004/12
N2 - Purpose: The current study was done to determine whether atorvastatin, the HMGCoA (3-hydroxy-3-methylglutaryl CoA) reductase inhibitor, could decrease renal transforming growth factor-β (TGF-β) levels in unilateral ureteral obstruction (UUO) and concomitantly affect renal tissue damage in UUO. Materials and Methods: Atorvastatin (20 mg/kg) was administered to rats 1 day prior to UUO and every day thereafter. Kidneys were harvested at day 14 after UUO. Tissue TGF-β was measured by bioassay using mink lung epithelial cells. Renal tubular proliferation and apoptosis were detected by immunostaining proliferating cell nuclear antigen and polyclonal antisingle strand DNA antibody, respectively. Fibrosis was assessed by measuring collagen deposition with trichrome stained slides. Interstitial leukocyte was detected by immunostaining CD45. Results: TGF-β bioassay showed that the obstructed kidney in the control group contained significantly higher TGF-β than the unobstructed kidney in the control group (mean ± SD 79.1 ± 48.5 vs 28.7 ± 13.7 pg/mg tissue) and atorvastatin significantly decrease tissue TGF-β in the obstructed kidney (53.4 ± 37.0 pg/mg tissue). Immunostaining polyclonal antisingle strand DNA antibody demonstrated that the obstructed kidney in the control group has significantly more tubular apoptosis than the unobstructed counterpart (4.8 ± 2.8 vs 2.1 ± 1.2 nuclei per high power field) and atorvastatin significantly decreased renal tubular apoptosis in the obstructed kidney (1.1 ± 0.7 nuclei per high power field). In addition, immunostaining proliferating cell nuclear antigen showed that the obstructed kidney in the atorvastatin group had significantly more renal tubular proliferation than the obstructed kidney in the control group (48.7 ± 20.8 vs 17.3 ± 10.6 per high power field). Control obstructed kidney showed significantly more fibrosis, which was also blunted by atorvastatin. Conclusions: Atorvastatin significantly decreases tissue TGF-β, resulting in a decrease in tubular damage and interstitial fibrosis. This suggests that atorvastatin is a promising agent for preventing renal tubular damage in UUO.
AB - Purpose: The current study was done to determine whether atorvastatin, the HMGCoA (3-hydroxy-3-methylglutaryl CoA) reductase inhibitor, could decrease renal transforming growth factor-β (TGF-β) levels in unilateral ureteral obstruction (UUO) and concomitantly affect renal tissue damage in UUO. Materials and Methods: Atorvastatin (20 mg/kg) was administered to rats 1 day prior to UUO and every day thereafter. Kidneys were harvested at day 14 after UUO. Tissue TGF-β was measured by bioassay using mink lung epithelial cells. Renal tubular proliferation and apoptosis were detected by immunostaining proliferating cell nuclear antigen and polyclonal antisingle strand DNA antibody, respectively. Fibrosis was assessed by measuring collagen deposition with trichrome stained slides. Interstitial leukocyte was detected by immunostaining CD45. Results: TGF-β bioassay showed that the obstructed kidney in the control group contained significantly higher TGF-β than the unobstructed kidney in the control group (mean ± SD 79.1 ± 48.5 vs 28.7 ± 13.7 pg/mg tissue) and atorvastatin significantly decrease tissue TGF-β in the obstructed kidney (53.4 ± 37.0 pg/mg tissue). Immunostaining polyclonal antisingle strand DNA antibody demonstrated that the obstructed kidney in the control group has significantly more tubular apoptosis than the unobstructed counterpart (4.8 ± 2.8 vs 2.1 ± 1.2 nuclei per high power field) and atorvastatin significantly decreased renal tubular apoptosis in the obstructed kidney (1.1 ± 0.7 nuclei per high power field). In addition, immunostaining proliferating cell nuclear antigen showed that the obstructed kidney in the atorvastatin group had significantly more renal tubular proliferation than the obstructed kidney in the control group (48.7 ± 20.8 vs 17.3 ± 10.6 per high power field). Control obstructed kidney showed significantly more fibrosis, which was also blunted by atorvastatin. Conclusions: Atorvastatin significantly decreases tissue TGF-β, resulting in a decrease in tubular damage and interstitial fibrosis. This suggests that atorvastatin is a promising agent for preventing renal tubular damage in UUO.
KW - Apoptosis
KW - Atorvastatin
KW - Kidney
KW - Transforming growth factor beta
KW - Ureteral obstruction
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U2 - 10.1097/01.ju.0000138473.38447.f0
DO - 10.1097/01.ju.0000138473.38447.f0
M3 - Article
VL - 172
SP - 2456
EP - 2459
JO - Journal of Urology
JF - Journal of Urology
SN - 0022-5347
IS - 6 I
ER -