Abstract
Hematopoietic stem cells (HSCs) are quiescent cells in the bone marrow niche and are relatively dependent on glycolytic ATP production. On the other hand, differentiated cells, including hematopoietic progenitor cells (HPCs), preferentially generate ATP via oxidative phosphorylation. However, it is unclear how cellular differentiation and the cell cycle status affect nutritional requirements and ATP production in HSCs and HPCs. Using a newly developed culture system, we demonstrated that survival of HPCs was strongly dependent on glucose, whereas quiescent HSCs survived for a certain duration without glucose. Among HPCs, granulocyte/monocyte progenitors (GMPs) were particularly dependent on glucose during proliferation. By monitoring the ATP concentration in live cells, we demonstrated that the ATP level was maintained for a short duration without glucose in HSCs, possibly due to their metabolic flexibility. In addition, HSCs exhibited low ATP turnover, whereas HPCs including GMPs demonstrated high ATP turnover and required efficient ATP production from glucose. These findings show that ATP turnover and nutritional requirements differ between HSCs and HPCs according to the cell cycle and differentiation status.
| Original language | English |
|---|---|
| Journal | Biochemical and Biophysical Research Communications |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
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Keywords
- Adenosine triphosphate
- Granulocyte/macrophage progenitor
- Hematopoietic stem cell
- Stem cell metabolism
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
ATP turnover and glucose dependency in hematopoietic stem/progenitor cells are increased by proliferation and differentiation. / Watanuki, Shintaro; Kobayashi, Hiroshi; Sorimachi, Yuriko; Yamamoto, Masamichi; Okamoto, Shinichiro; Takubo, Keiyo.
In: Biochemical and Biophysical Research Communications, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ATP turnover and glucose dependency in hematopoietic stem/progenitor cells are increased by proliferation and differentiation
AU - Watanuki, Shintaro
AU - Kobayashi, Hiroshi
AU - Sorimachi, Yuriko
AU - Yamamoto, Masamichi
AU - Okamoto, Shinichiro
AU - Takubo, Keiyo
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Hematopoietic stem cells (HSCs) are quiescent cells in the bone marrow niche and are relatively dependent on glycolytic ATP production. On the other hand, differentiated cells, including hematopoietic progenitor cells (HPCs), preferentially generate ATP via oxidative phosphorylation. However, it is unclear how cellular differentiation and the cell cycle status affect nutritional requirements and ATP production in HSCs and HPCs. Using a newly developed culture system, we demonstrated that survival of HPCs was strongly dependent on glucose, whereas quiescent HSCs survived for a certain duration without glucose. Among HPCs, granulocyte/monocyte progenitors (GMPs) were particularly dependent on glucose during proliferation. By monitoring the ATP concentration in live cells, we demonstrated that the ATP level was maintained for a short duration without glucose in HSCs, possibly due to their metabolic flexibility. In addition, HSCs exhibited low ATP turnover, whereas HPCs including GMPs demonstrated high ATP turnover and required efficient ATP production from glucose. These findings show that ATP turnover and nutritional requirements differ between HSCs and HPCs according to the cell cycle and differentiation status.
AB - Hematopoietic stem cells (HSCs) are quiescent cells in the bone marrow niche and are relatively dependent on glycolytic ATP production. On the other hand, differentiated cells, including hematopoietic progenitor cells (HPCs), preferentially generate ATP via oxidative phosphorylation. However, it is unclear how cellular differentiation and the cell cycle status affect nutritional requirements and ATP production in HSCs and HPCs. Using a newly developed culture system, we demonstrated that survival of HPCs was strongly dependent on glucose, whereas quiescent HSCs survived for a certain duration without glucose. Among HPCs, granulocyte/monocyte progenitors (GMPs) were particularly dependent on glucose during proliferation. By monitoring the ATP concentration in live cells, we demonstrated that the ATP level was maintained for a short duration without glucose in HSCs, possibly due to their metabolic flexibility. In addition, HSCs exhibited low ATP turnover, whereas HPCs including GMPs demonstrated high ATP turnover and required efficient ATP production from glucose. These findings show that ATP turnover and nutritional requirements differ between HSCs and HPCs according to the cell cycle and differentiation status.
KW - Adenosine triphosphate
KW - Granulocyte/macrophage progenitor
KW - Hematopoietic stem cell
KW - Stem cell metabolism
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UR - http://www.scopus.com/inward/citedby.url?scp=85064646726&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2019.04.123
DO - 10.1016/j.bbrc.2019.04.123
M3 - Article
C2 - 31030941
AN - SCOPUS:85064646726
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
ER -