Atrial natriuretic peptide polarizes human dendritic cells toward a Th2-promoting phenotype through its receptor guanylyl cyclase-coupled receptor A

Rimpei Morita, Naoya Ukyo, Mayumi Furuya, Takashi Uchiyama, Toshiyuki Hori

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Atrial natriuretic peptide (ANP) is a cardiovascular hormone secreted mainly by the cardiac atria and regulates the volume-pressure homeostasis. The action of ANP is mediated by its receptor, guanylyl cyclase-coupled receptor A (GC-A). In this study, we explored the possibility that ANP and GC-A may play a role in the dendritic cell (DC)-mediated immune regulation. We first examined the expression of GC-A in human monocyte-derived DCs in comparison with monocytes and found that DCs but not monocytes express GC-A at both the mRNA and protein levels. DCs responded to ANP with an increase in intracellular cGMP in a dose-dependent manner, indicating that GC-A expressed on DCs is functional. Furthermore, treatment of DCs with ANP decreased production of IL-12 and TNF-α and conversely increased that of IL-10 upon stimulation with LPS. In accordance with this change of cytokine production, DCs treated with ANP plus LPS promoted differentiation of naive CD4+ T cells into a Th2 phenotype. Finally, we presented evidence that ANP affected cytokine production of fresh whole blood stimulated with LPS in line with the above-mentioned results. These results indicate that ANP polarizes human DCs toward a Th2-promoting phenotype through GC-A and thus can regulate immune responses.

Original languageEnglish
Pages (from-to)5869-5875
Number of pages7
JournalJournal of Immunology
Volume170
Issue number12
Publication statusPublished - 2003 Jun 15
Externally publishedYes

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Atrial Natriuretic Factor
Dendritic Cells
Phenotype
Monocytes
Cytokines
atrial natriuretic factor receptor A
Interleukin-12
Interleukin-10
Homeostasis
Hormones
T-Lymphocytes
Pressure
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Atrial natriuretic peptide polarizes human dendritic cells toward a Th2-promoting phenotype through its receptor guanylyl cyclase-coupled receptor A. / Morita, Rimpei; Ukyo, Naoya; Furuya, Mayumi; Uchiyama, Takashi; Hori, Toshiyuki.

In: Journal of Immunology, Vol. 170, No. 12, 15.06.2003, p. 5869-5875.

Research output: Contribution to journalArticle

Morita, Rimpei ; Ukyo, Naoya ; Furuya, Mayumi ; Uchiyama, Takashi ; Hori, Toshiyuki. / Atrial natriuretic peptide polarizes human dendritic cells toward a Th2-promoting phenotype through its receptor guanylyl cyclase-coupled receptor A. In: Journal of Immunology. 2003 ; Vol. 170, No. 12. pp. 5869-5875.
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