Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy

Yoshihiko Saito, Kazuwa Nakao, Hiroshi Arai, Akira Sugawara, Narito Morii, Takayuki Yamada, Hiroshi Itoh, Shozo Shiono, Masashi Mukoyama, Kenji Obata, Hirofumi Yasue, Hiroaki Ohkubo, Shigetada Nakanishi, Hiroo Imura

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the human atrium and ventricle were measured simultaneously by the blot hybridization technique and the specific radioimmunoassay for ANP. Hearts were obtained from two patients without cardiac complications and from a patient with dilated cardiomyopathy (DCM) at autopsy. Total RNA extracted from ventricles contained a hydridizing RNA band of the same size as atrial ANPmRNA in both control and DCM hearts. The ANPmRNA level in the control ventricle was 0.2 % of that in the atrium. The ANPmRNA level in the DCM ventricle increased to about 7 % of that in the corresponding atrium and was approximately 40 times higher than that in the control ventricle, although the ANPmRNA level in the DCM atrium was comparable to that in the control atrium. The total content of ANPmRNA in the DCM ventricle reached about 30 % of that in the corresponding atrium and was much the same as that in the control atrium. The ANP level in the DCM ventricle was approximately 1.0 μg/g and much higher than that in the control ventricle (0.02 μg/g).

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number1
DOIs
Publication statusPublished - 1987 Oct 14
Externally publishedYes

Fingerprint

Dilated Cardiomyopathy
Gene expression
Atrial Natriuretic Factor
Gene Expression
Messenger RNA
Peptides
RNA
Radioimmunoassay
Autopsy
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy. / Saito, Yoshihiko; Nakao, Kazuwa; Arai, Hiroshi; Sugawara, Akira; Morii, Narito; Yamada, Takayuki; Itoh, Hiroshi; Shiono, Shozo; Mukoyama, Masashi; Obata, Kenji; Yasue, Hirofumi; Ohkubo, Hiroaki; Nakanishi, Shigetada; Imura, Hiroo.

In: Biochemical and Biophysical Research Communications, Vol. 148, No. 1, 14.10.1987, p. 211-217.

Research output: Contribution to journalArticle

Saito, Y, Nakao, K, Arai, H, Sugawara, A, Morii, N, Yamada, T, Itoh, H, Shiono, S, Mukoyama, M, Obata, K, Yasue, H, Ohkubo, H, Nakanishi, S & Imura, H 1987, 'Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy', Biochemical and Biophysical Research Communications, vol. 148, no. 1, pp. 211-217. https://doi.org/10.1016/0006-291X(87)91097-7
Saito, Yoshihiko ; Nakao, Kazuwa ; Arai, Hiroshi ; Sugawara, Akira ; Morii, Narito ; Yamada, Takayuki ; Itoh, Hiroshi ; Shiono, Shozo ; Mukoyama, Masashi ; Obata, Kenji ; Yasue, Hirofumi ; Ohkubo, Hiroaki ; Nakanishi, Shigetada ; Imura, Hiroo. / Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 148, No. 1. pp. 211-217.
@article{5a94e2b2db4f48ae97aed22223c5746a,
title = "Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy",
abstract = "Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the human atrium and ventricle were measured simultaneously by the blot hybridization technique and the specific radioimmunoassay for ANP. Hearts were obtained from two patients without cardiac complications and from a patient with dilated cardiomyopathy (DCM) at autopsy. Total RNA extracted from ventricles contained a hydridizing RNA band of the same size as atrial ANPmRNA in both control and DCM hearts. The ANPmRNA level in the control ventricle was 0.2 {\%} of that in the atrium. The ANPmRNA level in the DCM ventricle increased to about 7 {\%} of that in the corresponding atrium and was approximately 40 times higher than that in the control ventricle, although the ANPmRNA level in the DCM atrium was comparable to that in the control atrium. The total content of ANPmRNA in the DCM ventricle reached about 30 {\%} of that in the corresponding atrium and was much the same as that in the control atrium. The ANP level in the DCM ventricle was approximately 1.0 μg/g and much higher than that in the control ventricle (0.02 μg/g).",
author = "Yoshihiko Saito and Kazuwa Nakao and Hiroshi Arai and Akira Sugawara and Narito Morii and Takayuki Yamada and Hiroshi Itoh and Shozo Shiono and Masashi Mukoyama and Kenji Obata and Hirofumi Yasue and Hiroaki Ohkubo and Shigetada Nakanishi and Hiroo Imura",
year = "1987",
month = "10",
day = "14",
doi = "10.1016/0006-291X(87)91097-7",
language = "English",
volume = "148",
pages = "211--217",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy

AU - Saito, Yoshihiko

AU - Nakao, Kazuwa

AU - Arai, Hiroshi

AU - Sugawara, Akira

AU - Morii, Narito

AU - Yamada, Takayuki

AU - Itoh, Hiroshi

AU - Shiono, Shozo

AU - Mukoyama, Masashi

AU - Obata, Kenji

AU - Yasue, Hirofumi

AU - Ohkubo, Hiroaki

AU - Nakanishi, Shigetada

AU - Imura, Hiroo

PY - 1987/10/14

Y1 - 1987/10/14

N2 - Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the human atrium and ventricle were measured simultaneously by the blot hybridization technique and the specific radioimmunoassay for ANP. Hearts were obtained from two patients without cardiac complications and from a patient with dilated cardiomyopathy (DCM) at autopsy. Total RNA extracted from ventricles contained a hydridizing RNA band of the same size as atrial ANPmRNA in both control and DCM hearts. The ANPmRNA level in the control ventricle was 0.2 % of that in the atrium. The ANPmRNA level in the DCM ventricle increased to about 7 % of that in the corresponding atrium and was approximately 40 times higher than that in the control ventricle, although the ANPmRNA level in the DCM atrium was comparable to that in the control atrium. The total content of ANPmRNA in the DCM ventricle reached about 30 % of that in the corresponding atrium and was much the same as that in the control atrium. The ANP level in the DCM ventricle was approximately 1.0 μg/g and much higher than that in the control ventricle (0.02 μg/g).

AB - Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the human atrium and ventricle were measured simultaneously by the blot hybridization technique and the specific radioimmunoassay for ANP. Hearts were obtained from two patients without cardiac complications and from a patient with dilated cardiomyopathy (DCM) at autopsy. Total RNA extracted from ventricles contained a hydridizing RNA band of the same size as atrial ANPmRNA in both control and DCM hearts. The ANPmRNA level in the control ventricle was 0.2 % of that in the atrium. The ANPmRNA level in the DCM ventricle increased to about 7 % of that in the corresponding atrium and was approximately 40 times higher than that in the control ventricle, although the ANPmRNA level in the DCM atrium was comparable to that in the control atrium. The total content of ANPmRNA in the DCM ventricle reached about 30 % of that in the corresponding atrium and was much the same as that in the control atrium. The ANP level in the DCM ventricle was approximately 1.0 μg/g and much higher than that in the control ventricle (0.02 μg/g).

UR - http://www.scopus.com/inward/record.url?scp=0023616979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023616979&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(87)91097-7

DO - 10.1016/0006-291X(87)91097-7

M3 - Article

C2 - 2960322

AN - SCOPUS:0023616979

VL - 148

SP - 211

EP - 217

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -