TY - JOUR
T1 - Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy
AU - Saito, Yoshihiko
AU - Nakao, Kazuwa
AU - Arai, Hiroshi
AU - Sugawara, Akira
AU - Morii, Narito
AU - Yamada, Takayuki
AU - Itoh, Hiroshi
AU - Shiono, Shozo
AU - Mukoyama, Masashi
AU - Obata, Kenji
AU - Yasue, Hirofumi
AU - Ohkubo, Hiroaki
AU - Nakanishi, Shigetada
AU - Imura, Hiroo
N1 - Funding Information:
We acknowledge MS Tabata and Miss Furu for their secretarial assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare "Disorders of Adrenal Hormone" Research Committee, Japan, 1987, Japan Tobacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by a research grant for cardiovascular diseases (60A-3) and (62A'-1) from the Japanese Ministry Health and Welfare.
PY - 1987/10/14
Y1 - 1987/10/14
N2 - Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the human atrium and ventricle were measured simultaneously by the blot hybridization technique and the specific radioimmunoassay for ANP. Hearts were obtained from two patients without cardiac complications and from a patient with dilated cardiomyopathy (DCM) at autopsy. Total RNA extracted from ventricles contained a hydridizing RNA band of the same size as atrial ANPmRNA in both control and DCM hearts. The ANPmRNA level in the control ventricle was 0.2 % of that in the atrium. The ANPmRNA level in the DCM ventricle increased to about 7 % of that in the corresponding atrium and was approximately 40 times higher than that in the control ventricle, although the ANPmRNA level in the DCM atrium was comparable to that in the control atrium. The total content of ANPmRNA in the DCM ventricle reached about 30 % of that in the corresponding atrium and was much the same as that in the control atrium. The ANP level in the DCM ventricle was approximately 1.0 μg/g and much higher than that in the control ventricle (0.02 μg/g).
AB - Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the human atrium and ventricle were measured simultaneously by the blot hybridization technique and the specific radioimmunoassay for ANP. Hearts were obtained from two patients without cardiac complications and from a patient with dilated cardiomyopathy (DCM) at autopsy. Total RNA extracted from ventricles contained a hydridizing RNA band of the same size as atrial ANPmRNA in both control and DCM hearts. The ANPmRNA level in the control ventricle was 0.2 % of that in the atrium. The ANPmRNA level in the DCM ventricle increased to about 7 % of that in the corresponding atrium and was approximately 40 times higher than that in the control ventricle, although the ANPmRNA level in the DCM atrium was comparable to that in the control atrium. The total content of ANPmRNA in the DCM ventricle reached about 30 % of that in the corresponding atrium and was much the same as that in the control atrium. The ANP level in the DCM ventricle was approximately 1.0 μg/g and much higher than that in the control ventricle (0.02 μg/g).
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U2 - 10.1016/0006-291X(87)91097-7
DO - 10.1016/0006-291X(87)91097-7
M3 - Article
C2 - 2960322
AN - SCOPUS:0023616979
VL - 148
SP - 211
EP - 217
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -