Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy

Yoshihiko Saito, Kazuwa Nakao, Hiroshi Arai, Akira Sugawara, Narito Morii, Takayuki Yamada, Hiroshi Itoh, Shozo Shiono, Masashi Mukoyama, Kenji Obata, Hirofumi Yasue, Hiroaki Ohkubo, Shigetada Nakanishi, Hiroo Imura

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53 Citations (Scopus)

Abstract

Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the human atrium and ventricle were measured simultaneously by the blot hybridization technique and the specific radioimmunoassay for ANP. Hearts were obtained from two patients without cardiac complications and from a patient with dilated cardiomyopathy (DCM) at autopsy. Total RNA extracted from ventricles contained a hydridizing RNA band of the same size as atrial ANPmRNA in both control and DCM hearts. The ANPmRNA level in the control ventricle was 0.2 % of that in the atrium. The ANPmRNA level in the DCM ventricle increased to about 7 % of that in the corresponding atrium and was approximately 40 times higher than that in the control ventricle, although the ANPmRNA level in the DCM atrium was comparable to that in the control atrium. The total content of ANPmRNA in the DCM ventricle reached about 30 % of that in the corresponding atrium and was much the same as that in the control atrium. The ANP level in the DCM ventricle was approximately 1.0 μg/g and much higher than that in the control ventricle (0.02 μg/g).

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number1
DOIs
Publication statusPublished - 1987 Oct 14
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Saito, Y., Nakao, K., Arai, H., Sugawara, A., Morii, N., Yamada, T., Itoh, H., Shiono, S., Mukoyama, M., Obata, K., Yasue, H., Ohkubo, H., Nakanishi, S., & Imura, H. (1987). Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy. Biochemical and Biophysical Research Communications, 148(1), 211-217. https://doi.org/10.1016/0006-291X(87)91097-7