Atrial natriuretic polypeptide in spinal cord and autonomic ganglia

Narito Morii, Kazuwa Nakao, Hiroshi Itoh, Shozo Shiono, Takayuki Yamada, Akira Sugawara, Yoshihiko Saito, Masashi Mukoyama, Hiroshi Arai, Makoto Sakamoto, Hiroo Imura

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Abstract

Using a radioimmunoassay for α-rat atrial natriuretic polypeptide (α-rANP), tissue levels of α-rANP-like immunoreactivity (-LI) in the rat spinal cord and autonomic ganglia were investigated. The α-rANP-LI level was higher in the more caudal parts of the spinal cord and the highest in the sacral spinal cord. α-rANP-LI was also detected in the superior cervical and coeliac ganglia. Gel permeation chromatographic analysis showed that the major peak of α-rANP-LI in the spinal cord was a low molecular weight form co-eluted with synthetic α-rANP. Reverse-phase high performance liquid chromatographic analysis revealed that α-rANP-LI with a low molecular weight in the spinal cord consisted of several components, two major components of which co-migrated with synthetic α-rANP(4-28) and α-rANP(5-28), whereas little immunoreactivity was eluted at the position of α-rANP. These findings suggest the involvement of ANP in the function of the spinal cord and autonomic nervous system.

Original languageEnglish
Pages (from-to)196-203
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume145
Issue number1
DOIs
Publication statusPublished - 1987 May 29
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Morii, N., Nakao, K., Itoh, H., Shiono, S., Yamada, T., Sugawara, A., Saito, Y., Mukoyama, M., Arai, H., Sakamoto, M., & Imura, H. (1987). Atrial natriuretic polypeptide in spinal cord and autonomic ganglia. Biochemical and Biophysical Research Communications, 145(1), 196-203. https://doi.org/10.1016/0006-291X(87)91306-4