TY - JOUR
T1 - Attenuated chromatin compartmentalization in meiosis and its maturation in sperm development
AU - Alavattam, Kris G.
AU - Maezawa, So
AU - Sakashita, Akihiko
AU - Khoury, Haia
AU - Barski, Artem
AU - Kaplan, Noam
AU - Namekawa, Satoshi H.
N1 - Funding Information:
We thank J. Dekker, J. Gibcus, J. Taylor, M. Sauria, C. Price, J. Wolff, M. Yukawa, W. Deng, and R. Perea for advice and/or support during various stages of this project. We thank members of the Namekawa and Kaplan laboratories for discussion and helpful comments regarding the manuscript. Funding sources: Albert J. Ryan Fellowship to K.G.A.; National Institute of Health (NIH) R01 GM098605, R01 GM122776, and R21 ES027117, Research Grant (FY13-510) from the March of Dimes Foundation to S.H.N.; Azrieli Faculty Fellowship and Taub Fellowship to N.K.; NIH DP2 GM119134 to A.B.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Germ cells manifest a unique gene expression program and regain totipotency in the zygote. Here, we perform Hi-C analysis to examine 3D chromatin organization in male germ cells during spermatogenesis. We show that the highly compartmentalized 3D chromatin organization characteristic of interphase nuclei is attenuated in meiotic prophase. Meiotic prophase is predominated by short-range intrachromosomal interactions that represent a condensed form akin to that of mitotic chromosomes. However, unlike mitotic chromosomes, meiotic chromosomes display weak genomic compartmentalization, weak topologically associating domains, and localized point interactions in prophase. In postmeiotic round spermatids, genomic compartmentalization increases and gives rise to the strong compartmentalization seen in mature sperm. The X chromosome lacks domain organization during meiotic sex-chromosome inactivation. We propose that male meiosis occurs amid global reprogramming of 3D chromatin organization and that strengthening of chromatin compartmentalization takes place in spermiogenesis to prepare the next generation of life.
AB - Germ cells manifest a unique gene expression program and regain totipotency in the zygote. Here, we perform Hi-C analysis to examine 3D chromatin organization in male germ cells during spermatogenesis. We show that the highly compartmentalized 3D chromatin organization characteristic of interphase nuclei is attenuated in meiotic prophase. Meiotic prophase is predominated by short-range intrachromosomal interactions that represent a condensed form akin to that of mitotic chromosomes. However, unlike mitotic chromosomes, meiotic chromosomes display weak genomic compartmentalization, weak topologically associating domains, and localized point interactions in prophase. In postmeiotic round spermatids, genomic compartmentalization increases and gives rise to the strong compartmentalization seen in mature sperm. The X chromosome lacks domain organization during meiotic sex-chromosome inactivation. We propose that male meiosis occurs amid global reprogramming of 3D chromatin organization and that strengthening of chromatin compartmentalization takes place in spermiogenesis to prepare the next generation of life.
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U2 - 10.1038/s41594-019-0189-y
DO - 10.1038/s41594-019-0189-y
M3 - Article
C2 - 30778237
AN - SCOPUS:85061726199
VL - 26
SP - 175
EP - 184
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
SN - 1545-9993
IS - 3
ER -