Attenuation of hyperoxic lung injury by the 21-aminosteroid U-74389G

S. Tasaka, A. Ishizaka, T. Urano, K. Sayama, F. Sakamaki, H. Nakamura, T. Terashima, Y. Waki, Kenzo Soejima, Seitaro Fujishima, M. Kanazawa

Research output: Contribution to journalArticle

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Abstract

Hyperoxic lung injury is attributable to oxygen radicals produced under hyperoxic conditions. The 21-aminosteroid (AS), U-74389G, is a potent antioxidant. We examined the effect of U-74389G on lung injury in guinea pigs during exposure to 90% O2 for 48 h. We injected either vehicle or 10 mg/kg of U-74389G 30 min before the O2 exposure and injected the same dose 12, 24, and 36 h later. We performed two series of experiments after exposure. In the first series, we measured the clearance rate of (99m)Tc-labeled dialdehyde starch (DAS) from the lungs as an index of pulmonary epithelial damage in three experimental groups consisting of 1) control (n = 6), 2) O2 alone (n = 6), and 3) O2 + AS (n = 6). In the second series, pulmonary endothelial injury was estimated by using 28 guinea pigs divided into four experimental groups consisting of 1) control (n = 8), 2) AS alone (n = 5), 3) O2 alone (n = 6), and 4) O2 + AS (n = 9). In the second series, we measured the wet-to- dry weight ratio (W/D) as an index of lung water and the concentration ratio of 125I-labeled albumin in lung tissue and bronchoalveolar lavage (BAL) fluid compared with plasma (T/P and BAL/P, respectively) as indexes of pulmonary endothelial damage. Cell accumulation in BAL fluid and lung tissue samples was also assessed in the second series. In the O2 alone group, W/D, T/P, BAL/P, and DAS clearance rate were all increased compared with the control group (P < 0.05). The increases in W/D, BAL/P, and DAS clearance rate were attenuated by U-74389G treatment. Cell accumulation in the O2 alone group was also attenuated by U-74389G treatment. In conclusion, this study suggests that U-74389G attenuates lung injury induced by hyperoxic exposure.

Original languageEnglish
Pages (from-to)1635-1641
Number of pages7
JournalJournal of Applied Physiology
Volume78
Issue number5
Publication statusPublished - 1995

Fingerprint

Lung Injury
Bronchoalveolar Lavage Fluid
Bronchoalveolar Lavage
Lung
Guinea Pigs
U 74389F
Albumins
Reactive Oxygen Species
Antioxidants
Weights and Measures
Control Groups
Water
Therapeutics
dialdehyde starch

Keywords

  • endothelium
  • epithelium
  • hyperoxia
  • lung water

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Tasaka, S., Ishizaka, A., Urano, T., Sayama, K., Sakamaki, F., Nakamura, H., ... Kanazawa, M. (1995). Attenuation of hyperoxic lung injury by the 21-aminosteroid U-74389G. Journal of Applied Physiology, 78(5), 1635-1641.

Attenuation of hyperoxic lung injury by the 21-aminosteroid U-74389G. / Tasaka, S.; Ishizaka, A.; Urano, T.; Sayama, K.; Sakamaki, F.; Nakamura, H.; Terashima, T.; Waki, Y.; Soejima, Kenzo; Fujishima, Seitaro; Kanazawa, M.

In: Journal of Applied Physiology, Vol. 78, No. 5, 1995, p. 1635-1641.

Research output: Contribution to journalArticle

Tasaka, S, Ishizaka, A, Urano, T, Sayama, K, Sakamaki, F, Nakamura, H, Terashima, T, Waki, Y, Soejima, K, Fujishima, S & Kanazawa, M 1995, 'Attenuation of hyperoxic lung injury by the 21-aminosteroid U-74389G', Journal of Applied Physiology, vol. 78, no. 5, pp. 1635-1641.
Tasaka S, Ishizaka A, Urano T, Sayama K, Sakamaki F, Nakamura H et al. Attenuation of hyperoxic lung injury by the 21-aminosteroid U-74389G. Journal of Applied Physiology. 1995;78(5):1635-1641.
Tasaka, S. ; Ishizaka, A. ; Urano, T. ; Sayama, K. ; Sakamaki, F. ; Nakamura, H. ; Terashima, T. ; Waki, Y. ; Soejima, Kenzo ; Fujishima, Seitaro ; Kanazawa, M. / Attenuation of hyperoxic lung injury by the 21-aminosteroid U-74389G. In: Journal of Applied Physiology. 1995 ; Vol. 78, No. 5. pp. 1635-1641.
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