Augmentation of endotoxin-induced pulmonary responses by mononuclear cell phagocytosis in the reticuloendothelial system

Akitoshi Ishizaka, Naoki Hasegawa, Kouichi Sayama, Tetsuya Urano, Hidetoshi Nakamura, Fumio Sakamaki, Kenzo Soejima, Yasuhiro Waki, Sadatomo Tasaka, Morio Nakamura, Hiroaki Matsubara, Minoru Kanazawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: To test the hypothesis that the effects of intravenous injection of latex particles would demonstrate the contribution of phagocytosis by mononuclear phagocytes to the development of Escherichia coli-induced acute lung injury in neutropenic guinea pigs. Design: Prospective, controlled, experimental study. Intravenously injected the latex particles into 41 guinea pigs to investigate the contribution of the phagocytosis in acute lung injury. Subjects: Forty-one guinea pigs. Interventions: Forty-one guinea pigs were divided into five experimental groups: a saline group (n = 9); an endotoxin group (n = 10) receiving 2 mg/kg of intravenous E. coli endotoxin; a latex group (n = 7) receiving 2 x 109/kg of intravenous polystyrene latex (mean diameter 3.19 μm); an endotoxin + latex group (n = 8); and an E. coli group (n = 7) receiving 2 x 109 live E. coli/kg. Measurements and Main Results: The lung wet/dry ratio was increased in the live E. coli-treated guinea pigs (6.71±0.16 [SEM], p < .01) as compared with the saline control (5.40±0.16), whereas the ratio was not increased in the endotoxin (5.52±0.14) or latex (5.58±0.20) groups. However, the lung wet/dry ratio was greater in the endotoxin + latex group (6.11±0.16, p < .05) than in the saline control. The 125I albumin lung tissue/plasma ratio was greater in the E. coli (2.00±0.29, p < .01) and endotoxin + latex (0.84±0.12, p < .05) groups than in the saline group (0.18±0.07), whereas no increases were observed in the endotoxin group (0.22±0.10) and the latex (0.34±0.13) group. More than 40% of the injected radiolabeled latex was observed to have accumulated in the reticuloendothelial system (liver and spleen), in both the saline control (40.1 ±2.3%, n = 4) and endotoxin (57.3±6.8%, n = 5) groups, with 2.6± 1.5% and 3.1±1.7% in the lungs for the saline control and the endotoxin groups, respectively. The percent deposition of radiolabeled latex in the liver was greater in the endotoxin group (51.7± 3.8%, p < .05) than in the saline group (37.6±5.9%). Conclusions: These findings suggest that, in neutropenic guinea pigs: a) the combination of endotoxin and latex particles induces acute lung injury; and b) the phagocytic properties of mononuclear phagocytes in the reticuloendothelial system augment endotoxin-induced pulmonary responses and may play a role in the development of live E. coli-induced acute lung injury.

Original languageEnglish
Pages (from-to)1034-1040
Number of pages7
JournalCritical Care Medicine
Volume24
Issue number6
DOIs
Publication statusPublished - 1996 Jun

Fingerprint

Cytophagocytosis
Mononuclear Phagocyte System
Endotoxins
Latex
Lung
Guinea Pigs
Acute Lung Injury
Escherichia coli
Microspheres
Pulmonary Edema
Phagocytes
Phagocytosis
Liver
Intravenous Injections
Albumins
Spleen

Keywords

  • critical illness
  • endotoxin
  • latex
  • lung injury
  • neutrophil
  • phagocytes
  • pulmonary emergencies
  • reticuloendothelial system

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Augmentation of endotoxin-induced pulmonary responses by mononuclear cell phagocytosis in the reticuloendothelial system. / Ishizaka, Akitoshi; Hasegawa, Naoki; Sayama, Kouichi; Urano, Tetsuya; Nakamura, Hidetoshi; Sakamaki, Fumio; Soejima, Kenzo; Waki, Yasuhiro; Tasaka, Sadatomo; Nakamura, Morio; Matsubara, Hiroaki; Kanazawa, Minoru.

In: Critical Care Medicine, Vol. 24, No. 6, 06.1996, p. 1034-1040.

Research output: Contribution to journalArticle

Ishizaka, A, Hasegawa, N, Sayama, K, Urano, T, Nakamura, H, Sakamaki, F, Soejima, K, Waki, Y, Tasaka, S, Nakamura, M, Matsubara, H & Kanazawa, M 1996, 'Augmentation of endotoxin-induced pulmonary responses by mononuclear cell phagocytosis in the reticuloendothelial system', Critical Care Medicine, vol. 24, no. 6, pp. 1034-1040. https://doi.org/10.1097/00003246-199606000-00025
Ishizaka, Akitoshi ; Hasegawa, Naoki ; Sayama, Kouichi ; Urano, Tetsuya ; Nakamura, Hidetoshi ; Sakamaki, Fumio ; Soejima, Kenzo ; Waki, Yasuhiro ; Tasaka, Sadatomo ; Nakamura, Morio ; Matsubara, Hiroaki ; Kanazawa, Minoru. / Augmentation of endotoxin-induced pulmonary responses by mononuclear cell phagocytosis in the reticuloendothelial system. In: Critical Care Medicine. 1996 ; Vol. 24, No. 6. pp. 1034-1040.
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abstract = "Objective: To test the hypothesis that the effects of intravenous injection of latex particles would demonstrate the contribution of phagocytosis by mononuclear phagocytes to the development of Escherichia coli-induced acute lung injury in neutropenic guinea pigs. Design: Prospective, controlled, experimental study. Intravenously injected the latex particles into 41 guinea pigs to investigate the contribution of the phagocytosis in acute lung injury. Subjects: Forty-one guinea pigs. Interventions: Forty-one guinea pigs were divided into five experimental groups: a saline group (n = 9); an endotoxin group (n = 10) receiving 2 mg/kg of intravenous E. coli endotoxin; a latex group (n = 7) receiving 2 x 109/kg of intravenous polystyrene latex (mean diameter 3.19 μm); an endotoxin + latex group (n = 8); and an E. coli group (n = 7) receiving 2 x 109 live E. coli/kg. Measurements and Main Results: The lung wet/dry ratio was increased in the live E. coli-treated guinea pigs (6.71±0.16 [SEM], p < .01) as compared with the saline control (5.40±0.16), whereas the ratio was not increased in the endotoxin (5.52±0.14) or latex (5.58±0.20) groups. However, the lung wet/dry ratio was greater in the endotoxin + latex group (6.11±0.16, p < .05) than in the saline control. The 125I albumin lung tissue/plasma ratio was greater in the E. coli (2.00±0.29, p < .01) and endotoxin + latex (0.84±0.12, p < .05) groups than in the saline group (0.18±0.07), whereas no increases were observed in the endotoxin group (0.22±0.10) and the latex (0.34±0.13) group. More than 40{\%} of the injected radiolabeled latex was observed to have accumulated in the reticuloendothelial system (liver and spleen), in both the saline control (40.1 ±2.3{\%}, n = 4) and endotoxin (57.3±6.8{\%}, n = 5) groups, with 2.6± 1.5{\%} and 3.1±1.7{\%} in the lungs for the saline control and the endotoxin groups, respectively. The percent deposition of radiolabeled latex in the liver was greater in the endotoxin group (51.7± 3.8{\%}, p < .05) than in the saline group (37.6±5.9{\%}). Conclusions: These findings suggest that, in neutropenic guinea pigs: a) the combination of endotoxin and latex particles induces acute lung injury; and b) the phagocytic properties of mononuclear phagocytes in the reticuloendothelial system augment endotoxin-induced pulmonary responses and may play a role in the development of live E. coli-induced acute lung injury.",
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TY - JOUR

T1 - Augmentation of endotoxin-induced pulmonary responses by mononuclear cell phagocytosis in the reticuloendothelial system

AU - Ishizaka, Akitoshi

AU - Hasegawa, Naoki

AU - Sayama, Kouichi

AU - Urano, Tetsuya

AU - Nakamura, Hidetoshi

AU - Sakamaki, Fumio

AU - Soejima, Kenzo

AU - Waki, Yasuhiro

AU - Tasaka, Sadatomo

AU - Nakamura, Morio

AU - Matsubara, Hiroaki

AU - Kanazawa, Minoru

PY - 1996/6

Y1 - 1996/6

N2 - Objective: To test the hypothesis that the effects of intravenous injection of latex particles would demonstrate the contribution of phagocytosis by mononuclear phagocytes to the development of Escherichia coli-induced acute lung injury in neutropenic guinea pigs. Design: Prospective, controlled, experimental study. Intravenously injected the latex particles into 41 guinea pigs to investigate the contribution of the phagocytosis in acute lung injury. Subjects: Forty-one guinea pigs. Interventions: Forty-one guinea pigs were divided into five experimental groups: a saline group (n = 9); an endotoxin group (n = 10) receiving 2 mg/kg of intravenous E. coli endotoxin; a latex group (n = 7) receiving 2 x 109/kg of intravenous polystyrene latex (mean diameter 3.19 μm); an endotoxin + latex group (n = 8); and an E. coli group (n = 7) receiving 2 x 109 live E. coli/kg. Measurements and Main Results: The lung wet/dry ratio was increased in the live E. coli-treated guinea pigs (6.71±0.16 [SEM], p < .01) as compared with the saline control (5.40±0.16), whereas the ratio was not increased in the endotoxin (5.52±0.14) or latex (5.58±0.20) groups. However, the lung wet/dry ratio was greater in the endotoxin + latex group (6.11±0.16, p < .05) than in the saline control. The 125I albumin lung tissue/plasma ratio was greater in the E. coli (2.00±0.29, p < .01) and endotoxin + latex (0.84±0.12, p < .05) groups than in the saline group (0.18±0.07), whereas no increases were observed in the endotoxin group (0.22±0.10) and the latex (0.34±0.13) group. More than 40% of the injected radiolabeled latex was observed to have accumulated in the reticuloendothelial system (liver and spleen), in both the saline control (40.1 ±2.3%, n = 4) and endotoxin (57.3±6.8%, n = 5) groups, with 2.6± 1.5% and 3.1±1.7% in the lungs for the saline control and the endotoxin groups, respectively. The percent deposition of radiolabeled latex in the liver was greater in the endotoxin group (51.7± 3.8%, p < .05) than in the saline group (37.6±5.9%). Conclusions: These findings suggest that, in neutropenic guinea pigs: a) the combination of endotoxin and latex particles induces acute lung injury; and b) the phagocytic properties of mononuclear phagocytes in the reticuloendothelial system augment endotoxin-induced pulmonary responses and may play a role in the development of live E. coli-induced acute lung injury.

AB - Objective: To test the hypothesis that the effects of intravenous injection of latex particles would demonstrate the contribution of phagocytosis by mononuclear phagocytes to the development of Escherichia coli-induced acute lung injury in neutropenic guinea pigs. Design: Prospective, controlled, experimental study. Intravenously injected the latex particles into 41 guinea pigs to investigate the contribution of the phagocytosis in acute lung injury. Subjects: Forty-one guinea pigs. Interventions: Forty-one guinea pigs were divided into five experimental groups: a saline group (n = 9); an endotoxin group (n = 10) receiving 2 mg/kg of intravenous E. coli endotoxin; a latex group (n = 7) receiving 2 x 109/kg of intravenous polystyrene latex (mean diameter 3.19 μm); an endotoxin + latex group (n = 8); and an E. coli group (n = 7) receiving 2 x 109 live E. coli/kg. Measurements and Main Results: The lung wet/dry ratio was increased in the live E. coli-treated guinea pigs (6.71±0.16 [SEM], p < .01) as compared with the saline control (5.40±0.16), whereas the ratio was not increased in the endotoxin (5.52±0.14) or latex (5.58±0.20) groups. However, the lung wet/dry ratio was greater in the endotoxin + latex group (6.11±0.16, p < .05) than in the saline control. The 125I albumin lung tissue/plasma ratio was greater in the E. coli (2.00±0.29, p < .01) and endotoxin + latex (0.84±0.12, p < .05) groups than in the saline group (0.18±0.07), whereas no increases were observed in the endotoxin group (0.22±0.10) and the latex (0.34±0.13) group. More than 40% of the injected radiolabeled latex was observed to have accumulated in the reticuloendothelial system (liver and spleen), in both the saline control (40.1 ±2.3%, n = 4) and endotoxin (57.3±6.8%, n = 5) groups, with 2.6± 1.5% and 3.1±1.7% in the lungs for the saline control and the endotoxin groups, respectively. The percent deposition of radiolabeled latex in the liver was greater in the endotoxin group (51.7± 3.8%, p < .05) than in the saline group (37.6±5.9%). Conclusions: These findings suggest that, in neutropenic guinea pigs: a) the combination of endotoxin and latex particles induces acute lung injury; and b) the phagocytic properties of mononuclear phagocytes in the reticuloendothelial system augment endotoxin-induced pulmonary responses and may play a role in the development of live E. coli-induced acute lung injury.

KW - critical illness

KW - endotoxin

KW - latex

KW - lung injury

KW - neutrophil

KW - phagocytes

KW - pulmonary emergencies

KW - reticuloendothelial system

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