Abstract
Objective. The functions of a small GTP-binding protein, Rho, in human leukemic cell invasion was investigated in vivo and in vitro. Materials and Methods. Human leukemic KM3 and Reh cells (derived from B-cell-type common acute lymphoid leukemias) were inoculated into severe combined immundeficiency (SCID) mice. Alteration of invasion in SCID mice inoculated with KM3 cells that were introduced with the expression vector for Rho Val14 (Rho V14), an activated mutant form of Rho, was observed. Results. SCID mice inoculated with KM3 and Reh cells developed paraplegia 21 days after inoculation. All died by day 26-27. The leukemic cells were localized to bone marrow and around the spinal cord, with no infiltration into peripheral blood, spleen, liver, thymus, or lymph nodes. SCID mice inoculated with Rho V14-transfected KM3 cells showed a 5-day reduction in the time to paraplegia and death compared with SCID mice inoculated with hygromycin-resistance gene-transfected KM3 (hyg(r)) cells. In addition, the mice inoculated with Rho V14 cells showed leukemic cell infiltration, not only into bone marrow and around the spinal cord but also into peripheral blood, liver, and spleen. There were no in vitro or in vivo differences in growth rates of Rho V14 and hyg(r) cells. However, the Rho V14 cells showed markedly increased cell adhesion compared to the hyg(r) cells. Conclusion. Results suggest that Rho activation accelerates human leukemic cell invasion via augmentation of cell adhesion. Copyright (C) 2000 International Society for Experimental Hematology.
| Original language | English |
|---|---|
| Pages (from-to) | 391-400 |
| Number of pages | 10 |
| Journal | Experimental Hematology |
| Volume | 28 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2000 Apr |
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Keywords
- Cell adhesion
- Invasion
- Leukemia
- Rho
- SCID mice
ASJC Scopus subject areas
- Cancer Research
- Cell Biology
- Genetics
- Hematology
- Oncology
- Transplantation
Cite this
Augmentation of human leukemic cell invasion by activation of a small GTP-binding protein Rho. / Fukushima, Sachiko; Yamada, Taketo; Hashiguchi, Akinori; Nakata, Yuji; Hata, Jun ichi.
In: Experimental Hematology, Vol. 28, No. 4, 04.2000, p. 391-400.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Augmentation of human leukemic cell invasion by activation of a small GTP-binding protein Rho
AU - Fukushima, Sachiko
AU - Yamada, Taketo
AU - Hashiguchi, Akinori
AU - Nakata, Yuji
AU - Hata, Jun ichi
PY - 2000/4
Y1 - 2000/4
N2 - Objective. The functions of a small GTP-binding protein, Rho, in human leukemic cell invasion was investigated in vivo and in vitro. Materials and Methods. Human leukemic KM3 and Reh cells (derived from B-cell-type common acute lymphoid leukemias) were inoculated into severe combined immundeficiency (SCID) mice. Alteration of invasion in SCID mice inoculated with KM3 cells that were introduced with the expression vector for Rho Val14 (Rho V14), an activated mutant form of Rho, was observed. Results. SCID mice inoculated with KM3 and Reh cells developed paraplegia 21 days after inoculation. All died by day 26-27. The leukemic cells were localized to bone marrow and around the spinal cord, with no infiltration into peripheral blood, spleen, liver, thymus, or lymph nodes. SCID mice inoculated with Rho V14-transfected KM3 cells showed a 5-day reduction in the time to paraplegia and death compared with SCID mice inoculated with hygromycin-resistance gene-transfected KM3 (hyg(r)) cells. In addition, the mice inoculated with Rho V14 cells showed leukemic cell infiltration, not only into bone marrow and around the spinal cord but also into peripheral blood, liver, and spleen. There were no in vitro or in vivo differences in growth rates of Rho V14 and hyg(r) cells. However, the Rho V14 cells showed markedly increased cell adhesion compared to the hyg(r) cells. Conclusion. Results suggest that Rho activation accelerates human leukemic cell invasion via augmentation of cell adhesion. Copyright (C) 2000 International Society for Experimental Hematology.
AB - Objective. The functions of a small GTP-binding protein, Rho, in human leukemic cell invasion was investigated in vivo and in vitro. Materials and Methods. Human leukemic KM3 and Reh cells (derived from B-cell-type common acute lymphoid leukemias) were inoculated into severe combined immundeficiency (SCID) mice. Alteration of invasion in SCID mice inoculated with KM3 cells that were introduced with the expression vector for Rho Val14 (Rho V14), an activated mutant form of Rho, was observed. Results. SCID mice inoculated with KM3 and Reh cells developed paraplegia 21 days after inoculation. All died by day 26-27. The leukemic cells were localized to bone marrow and around the spinal cord, with no infiltration into peripheral blood, spleen, liver, thymus, or lymph nodes. SCID mice inoculated with Rho V14-transfected KM3 cells showed a 5-day reduction in the time to paraplegia and death compared with SCID mice inoculated with hygromycin-resistance gene-transfected KM3 (hyg(r)) cells. In addition, the mice inoculated with Rho V14 cells showed leukemic cell infiltration, not only into bone marrow and around the spinal cord but also into peripheral blood, liver, and spleen. There were no in vitro or in vivo differences in growth rates of Rho V14 and hyg(r) cells. However, the Rho V14 cells showed markedly increased cell adhesion compared to the hyg(r) cells. Conclusion. Results suggest that Rho activation accelerates human leukemic cell invasion via augmentation of cell adhesion. Copyright (C) 2000 International Society for Experimental Hematology.
KW - Cell adhesion
KW - Invasion
KW - Leukemia
KW - Rho
KW - SCID mice
UR - http://www.scopus.com/inward/record.url?scp=0034009104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034009104&partnerID=8YFLogxK
U2 - 10.1016/S0301-472X(00)00129-6
DO - 10.1016/S0301-472X(00)00129-6
M3 - Article
C2 - 10781897
AN - SCOPUS:0034009104
VL - 28
SP - 391
EP - 400
JO - Experimental Hematology
JF - Experimental Hematology
SN - 0301-472X
IS - 4
ER -