Augmented expression of the endothelin-A receptor gene in cultured mesangial cells from stroke-prone spontaneously hypertensive rats

J. Hiraoka, H. Arai, T. Yoshimasa, K. Takaya, Y. Miyamoto, J. Yamashita, S. I. Suga, Y. Ogawa, G. Shirakami, Hiroshi Itoh, Y. Yamori, K. Nakao

Research output: Contribution to journalArticle

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Abstract

1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET-A receptor (ET-AR) gene and the ET-B receptor (ET-BR) gene in cultured mesangial cells isolated from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET-AR predominantly. The level of the ET-AR mRNA in mesangial cells from SHRSP was 5-fold higher than that in the cells from WKY. The ET-BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET-AR gene was markedly augmented in mesangial cells from SHRSP.

Original languageEnglish
JournalClinical and Experimental Pharmacology and Physiology
Volume22
Issue numberSUPPL. 1
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Endothelin A Receptors
Mesangial Cells
Inbred SHR Rats
Endothelins
Cultured Cells
Stroke
Genes
Endothelin B Receptors
Endothelin Receptors
Messenger RNA
Inbred WKY Rats
Northern Blotting
Hypertension

Keywords

  • Endothelin
  • Endothelin receptor
  • Gene expression
  • Glomerular mesangial cells
  • Hypertension
  • Stroke-prone spontaneously hypertensive rat
  • Wistar-Kyoto rat

ASJC Scopus subject areas

  • Physiology
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Augmented expression of the endothelin-A receptor gene in cultured mesangial cells from stroke-prone spontaneously hypertensive rats. / Hiraoka, J.; Arai, H.; Yoshimasa, T.; Takaya, K.; Miyamoto, Y.; Yamashita, J.; Suga, S. I.; Ogawa, Y.; Shirakami, G.; Itoh, Hiroshi; Yamori, Y.; Nakao, K.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 22, No. SUPPL. 1, 1995.

Research output: Contribution to journalArticle

Hiraoka, J, Arai, H, Yoshimasa, T, Takaya, K, Miyamoto, Y, Yamashita, J, Suga, SI, Ogawa, Y, Shirakami, G, Itoh, H, Yamori, Y & Nakao, K 1995, 'Augmented expression of the endothelin-A receptor gene in cultured mesangial cells from stroke-prone spontaneously hypertensive rats', Clinical and Experimental Pharmacology and Physiology, vol. 22, no. SUPPL. 1.
Hiraoka, J. ; Arai, H. ; Yoshimasa, T. ; Takaya, K. ; Miyamoto, Y. ; Yamashita, J. ; Suga, S. I. ; Ogawa, Y. ; Shirakami, G. ; Itoh, Hiroshi ; Yamori, Y. ; Nakao, K. / Augmented expression of the endothelin-A receptor gene in cultured mesangial cells from stroke-prone spontaneously hypertensive rats. In: Clinical and Experimental Pharmacology and Physiology. 1995 ; Vol. 22, No. SUPPL. 1.
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T1 - Augmented expression of the endothelin-A receptor gene in cultured mesangial cells from stroke-prone spontaneously hypertensive rats

AU - Hiraoka, J.

AU - Arai, H.

AU - Yoshimasa, T.

AU - Takaya, K.

AU - Miyamoto, Y.

AU - Yamashita, J.

AU - Suga, S. I.

AU - Ogawa, Y.

AU - Shirakami, G.

AU - Itoh, Hiroshi

AU - Yamori, Y.

AU - Nakao, K.

PY - 1995

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N2 - 1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET-A receptor (ET-AR) gene and the ET-B receptor (ET-BR) gene in cultured mesangial cells isolated from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET-AR predominantly. The level of the ET-AR mRNA in mesangial cells from SHRSP was 5-fold higher than that in the cells from WKY. The ET-BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET-AR gene was markedly augmented in mesangial cells from SHRSP.

AB - 1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET-A receptor (ET-AR) gene and the ET-B receptor (ET-BR) gene in cultured mesangial cells isolated from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET-AR predominantly. The level of the ET-AR mRNA in mesangial cells from SHRSP was 5-fold higher than that in the cells from WKY. The ET-BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET-AR gene was markedly augmented in mesangial cells from SHRSP.

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KW - Endothelin receptor

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KW - Stroke-prone spontaneously hypertensive rat

KW - Wistar-Kyoto rat

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