AUGMENTED EXPRESSION OF THE ENDOTHELIN‐A RECEPTOR GENE IN CULTURED MESANGIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS

Junko Hiraoka; Hiroshi Arai; Takaaki Yoshimasa; Kazuhiko Takaya; Yoshihiro Miyamoto; Jun Yamashita; Shin‐ichi ‐i Suga; Yoshihiro Ogawa; Gotaro Shirakami; Hiroshi Itoh; Yukio Yamori; Kazuwa Nakao

doi:10.1111/j.1440-1681.1995.tb02876.x

AUGMENTED EXPRESSION OF THE ENDOTHELIN‐A RECEPTOR GENE IN CULTURED MESANGIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS

Junko Hiraoka, Hiroshi Arai, Takaaki Yoshimasa, Kazuhiko Takaya, Yoshihiro Miyamoto, Jun Yamashita, Shin‐ichi ‐i Suga, Yoshihiro Ogawa, Gotaro Shirakami, Hiroshi Itoh, Yukio Yamori, Kazuwa Nakao

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET‐A receptor (ET‐ AR) gene and the ET‐B receptor (ET‐BR) gene in cultured mesangial cells isolated from stroke‐prone spontaneously hypertensive rats (SHRSP) and Wistar‐Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET‐AR predominantly. The level of the ET‐AR mRNA in mesangial cells from SHRSP was 5‐fold higher than that in the cells from WKY. The ET‐BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET‐AR gene was markedly augmented in mesangial cells from SHRSP.

Original language	English
Pages (from-to)	S191-S192
Journal	Clinical and Experimental Pharmacology and Physiology
Volume	22
DOIs	https://doi.org/10.1111/j.1440-1681.1995.tb02876.x
Publication status	Published - 1995 Nov
Externally published	Yes

Keywords

Wistar‐Kyoto rat
endothelin
endothelin receptor
gene expression
glomerular mesangial cells
hypertension
stroke‐prone spontaneously hypertensive rat

ASJC Scopus subject areas

Physiology
Pharmacology
Physiology (medical)

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Hiraoka, J., Arai, H., Yoshimasa, T., Takaya, K., Miyamoto, Y., Yamashita, J., Suga, S. ., Ogawa, Y., Shirakami, G., Itoh, H., Yamori, Y., & Nakao, K. (1995). AUGMENTED EXPRESSION OF THE ENDOTHELIN‐A RECEPTOR GENE IN CULTURED MESANGIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS. Clinical and Experimental Pharmacology and Physiology, 22, S191-S192. https://doi.org/10.1111/j.1440-1681.1995.tb02876.x

AUGMENTED EXPRESSION OF THE ENDOTHELIN‐A RECEPTOR GENE IN CULTURED MESANGIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS. / Hiraoka, Junko; Arai, Hiroshi; Yoshimasa, Takaaki; Takaya, Kazuhiko; Miyamoto, Yoshihiro; Yamashita, Jun; Suga, Shin‐ichi ‐i; Ogawa, Yoshihiro; Shirakami, Gotaro; Itoh, Hiroshi; Yamori, Yukio; Nakao, Kazuwa.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 22, 11.1995, p. S191-S192.

Research output: Contribution to journal › Article

Hiraoka, J, Arai, H, Yoshimasa, T, Takaya, K, Miyamoto, Y, Yamashita, J, Suga, S, Ogawa, Y, Shirakami, G, Itoh, H, Yamori, Y & Nakao, K 1995, 'AUGMENTED EXPRESSION OF THE ENDOTHELIN‐A RECEPTOR GENE IN CULTURED MESANGIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS', Clinical and Experimental Pharmacology and Physiology, vol. 22, pp. S191-S192. https://doi.org/10.1111/j.1440-1681.1995.tb02876.x

Hiraoka, Junko ; Arai, Hiroshi ; Yoshimasa, Takaaki ; Takaya, Kazuhiko ; Miyamoto, Yoshihiro ; Yamashita, Jun ; Suga, Shin‐ichi ‐i ; Ogawa, Yoshihiro ; Shirakami, Gotaro ; Itoh, Hiroshi ; Yamori, Yukio ; Nakao, Kazuwa. / AUGMENTED EXPRESSION OF THE ENDOTHELIN‐A RECEPTOR GENE IN CULTURED MESANGIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS. In: Clinical and Experimental Pharmacology and Physiology. 1995 ; Vol. 22. pp. S191-S192.

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abstract = "1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET‐A receptor (ET‐ AR) gene and the ET‐B receptor (ET‐BR) gene in cultured mesangial cells isolated from stroke‐prone spontaneously hypertensive rats (SHRSP) and Wistar‐Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET‐AR predominantly. The level of the ET‐AR mRNA in mesangial cells from SHRSP was 5‐fold higher than that in the cells from WKY. The ET‐BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET‐AR gene was markedly augmented in mesangial cells from SHRSP.",

keywords = "Wistar‐Kyoto rat, endothelin, endothelin receptor, gene expression, glomerular mesangial cells, hypertension, stroke‐prone spontaneously hypertensive rat",

author = "Junko Hiraoka and Hiroshi Arai and Takaaki Yoshimasa and Kazuhiko Takaya and Yoshihiro Miyamoto and Jun Yamashita and Suga, {Shin‐ichi ‐i} and Yoshihiro Ogawa and Gotaro Shirakami and Hiroshi Itoh and Yukio Yamori and Kazuwa Nakao",

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doi = "10.1111/j.1440-1681.1995.tb02876.x",

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AU - Hiraoka, Junko

AU - Arai, Hiroshi

AU - Yoshimasa, Takaaki

AU - Takaya, Kazuhiko

AU - Miyamoto, Yoshihiro

AU - Yamashita, Jun

AU - Suga, Shin‐ichi ‐i

AU - Ogawa, Yoshihiro

AU - Shirakami, Gotaro

AU - Itoh, Hiroshi

AU - Yamori, Yukio

AU - Nakao, Kazuwa

PY - 1995/11

Y1 - 1995/11

N2 - 1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET‐A receptor (ET‐ AR) gene and the ET‐B receptor (ET‐BR) gene in cultured mesangial cells isolated from stroke‐prone spontaneously hypertensive rats (SHRSP) and Wistar‐Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET‐AR predominantly. The level of the ET‐AR mRNA in mesangial cells from SHRSP was 5‐fold higher than that in the cells from WKY. The ET‐BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET‐AR gene was markedly augmented in mesangial cells from SHRSP.

AB - 1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET‐A receptor (ET‐ AR) gene and the ET‐B receptor (ET‐BR) gene in cultured mesangial cells isolated from stroke‐prone spontaneously hypertensive rats (SHRSP) and Wistar‐Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET‐AR predominantly. The level of the ET‐AR mRNA in mesangial cells from SHRSP was 5‐fold higher than that in the cells from WKY. The ET‐BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET‐AR gene was markedly augmented in mesangial cells from SHRSP.

KW - Wistar‐Kyoto rat

KW - endothelin

KW - endothelin receptor

KW - gene expression

KW - glomerular mesangial cells

KW - hypertension

KW - stroke‐prone spontaneously hypertensive rat

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