@article{bdf6163ca4cb4fe68c7d9ed8670c2d54,
title = "Auto-reactive B cells against peripheral antigen, desmoglein 3, escape from tolerance mechanism",
abstract = "To examine the mechanism of B cell tolerance against natural peripheral self-antigen, we generated transgenic mice expressing IgM specific for desmoglein 3 (Dsg3) from AK7 monoclonal antibody which itself does not induce blisters. Dsg3 is mainly expressed on stratified squamous epithelium and is the target antigen of an autoimmune bullous disease, pemphigus vulgaris. Transgenic B cells reactive to Dsg3 were observed in the spleen and lymph node. Although these B cells are autoreactive, they did not develop into B1 B cells. These B cells were functionally competent and anti-Dsg3 IgM was detected in the serum and on the keratinocyte cell surface. These results indicate that auto-reactive B cells against peripheral antigen (Dsg3) are able to develop in the presence of Dsg3 but are ignored by the immune system.",
keywords = "Autoimmunity, B1 B cell, Pemphigus vulgaris, Peripheral tolerance, Transgenic mouse",
author = "Takayuki Ota and Miyo Aoki-Ota and Kazuyuki Tsunoda and Kouji Simoda and Takeji Nishikawa and Masayuki Amagai and Shigeo Koyasu",
note = "Funding Information: We are grateful to M. Motouchi, K. Furuichi and N. Yumoto for their excellent animal care. We also thank Drs H. Karasuyama, K. Minegishi and Linda Clayton for critical reading of the manuscript. This work was supported by a Keio University Special Grant-in-Aid for Innovative Collaborative Research Projects, a Grant-in-Aid for Scientific Research (B) (13557026) and a Grant-in-Aid for Creative Scientific Research (13GS0015) from the Japan Society for the Promotion of Science, a National Grant-in-Aid for the Establishment of a High-Tech Research Center in a Private University, a grant for the Promotion of the Advancement of Education and Research in Graduate Schools, and a Scientific Frontier Research Grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and Health and Labor Sciences Research Grants for Research on Measures for Intractable Diseases, from the Ministry of Health, Labor, and Welfare of Japan.",
year = "2004",
month = oct,
doi = "10.1093/intimm/dxh150",
language = "English",
volume = "16",
pages = "1487--1495",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "10",
}