Auto-reactive B cells against peripheral antigen, desmoglein 3, escape from tolerance mechanism

Takayuki Ota, Miyo Aoki-Ota, Kazuyuki Tsunoda, Kouji Simoda, Takeji Nishikawa, Masayuki Amagai, Shigeo Koyasu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To examine the mechanism of B cell tolerance against natural peripheral self-antigen, we generated transgenic mice expressing IgM specific for desmoglein 3 (Dsg3) from AK7 monoclonal antibody which itself does not induce blisters. Dsg3 is mainly expressed on stratified squamous epithelium and is the target antigen of an autoimmune bullous disease, pemphigus vulgaris. Transgenic B cells reactive to Dsg3 were observed in the spleen and lymph node. Although these B cells are autoreactive, they did not develop into B1 B cells. These B cells were functionally competent and anti-Dsg3 IgM was detected in the serum and on the keratinocyte cell surface. These results indicate that auto-reactive B cells against peripheral antigen (Dsg3) are able to develop in the presence of Dsg3 but are ignored by the immune system.

Original languageEnglish
Pages (from-to)1487-1495
Number of pages9
JournalInternational immunology
Volume16
Issue number10
DOIs
Publication statusPublished - 2004 Oct 1

Keywords

  • Autoimmunity
  • B1 B cell
  • Pemphigus vulgaris
  • Peripheral tolerance
  • Transgenic mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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