Autoantibodies against cell adhesion molecules in pemphigus

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

cDNA cloning has demonstrated that pemphigus autoantigens of both pemphigus vulgaris (PV) and pemphigus foliaceus are members of the desmoglein subfamily of the cadherin supergene family. The availability of these cDNAs allowed us to utilize molecular engineering to attempt to understand the pathophysiology of pemphigus. Transfection study with a chimeric molecule containing the extracellular domain of PV antigen (PVA) and the cytoplasmic domain of E-cadherin demonstrated that the extracellular domain of PVA mediates weak hemophilic cell adhesion. Bacterial fusion proteins representing different parts of PVA showed that the major immunogenic domains are EC1, EC2, and EC4 and that at least one pathogenic epitope is located on the amino-terminal region of PVA, an area thought to be important for classic cadherin hemophilic interaction. Further, a secreted form of PVA recombinant protein, PVIg, was produced by baculovirus expression. Immunoabsorption assay has demonstrated that PVIg is capable of absorbing pathogenic autoantibodies from patients' sera and preventing blister formation in neonatal mice.

Original languageEnglish
Pages (from-to)833-837
Number of pages5
JournalJournal of Dermatology
Volume21
Issue number11
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Pemphigus
Cell Adhesion Molecules
Autoantibodies
Antigens
Cadherins
Desmogleins
Complementary DNA
Bacterial Proteins
Baculoviridae
Autoantigens
Blister
Recombinant Proteins
Cell Adhesion
Transfection
Organism Cloning
Epitopes
Serum

ASJC Scopus subject areas

  • Dermatology

Cite this

Autoantibodies against cell adhesion molecules in pemphigus. / Amagai, Masayuki.

In: Journal of Dermatology, Vol. 21, No. 11, 1994, p. 833-837.

Research output: Contribution to journalArticle

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