Autoantibodies to glycyl-transfer RNA synthetase in myositis

Michito Hirakata, Akira Suwa, Yoshihiko Takeda, Yasuo Matsuoka, Shoichiro Irimajiri, Ira N. Targoff, John A. Hardin, Joe Craft

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective. To elucidate the clinical significance and immunologic heterogeneity of anti-glycyl-transfer RNA (tRNA) synthetase antibodies in polymyositis/ dermatomyositis (PM/DM). Methods. Sera from 345 patients with rheumatic diseases, including 91 with myositis, were examined using immunoprecipitation assays. Autoantibodies to aminoacyl-tRNA synthetases were further analyzed with 2-dimensional RNA fractionation and via inhibition of in vitro aminoacylation. Results. Serum from 1 patient with DM and interstitial lung disease immunoprecipitated glycyl-tRNA synthetase along with only 1 of 4 associated tRNAs, in comparison with control anti-glycyl-tRNA synthetase antibodies, which bound the enzyme along with all 4 associated tRNAs. Immunoblotting findings and a lack of in vitro inhibition aminoacylation of tRNAgly by serum from this patient also suggested differences between the epitope specificity of this serum and that of other sera with anti-glycyl-tRNA synthetase antibodies. Conclusion. This identification of antibodies to glycyl-tRNA synthetase from a patient with DM underscores the association of this specificity with the disease. The finding that these antibodies bound an epitope outside the active site of the synthetase enzyme, in contrast to most anti-aminoacyl-tRNA synthetases, emphasizes the immunologic heterogeneity of these autoantibodies.

Original languageEnglish
Pages (from-to)146-151
Number of pages6
JournalArthritis and Rheumatism
Volume39
Issue number1
Publication statusPublished - 1996 Jan

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Amino Acyl-tRNA Synthetases
Myositis
Autoantibodies
Antibodies
Aminoacylation
Serum
Transfer RNA
Epitopes
Dermatomyositis
Interstitial Lung Diseases
Enzymes
Ligases
Rheumatic Diseases
Immunoprecipitation
Immunoblotting
Catalytic Domain
RNA

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Hirakata, M., Suwa, A., Takeda, Y., Matsuoka, Y., Irimajiri, S., Targoff, I. N., ... Craft, J. (1996). Autoantibodies to glycyl-transfer RNA synthetase in myositis. Arthritis and Rheumatism, 39(1), 146-151.

Autoantibodies to glycyl-transfer RNA synthetase in myositis. / Hirakata, Michito; Suwa, Akira; Takeda, Yoshihiko; Matsuoka, Yasuo; Irimajiri, Shoichiro; Targoff, Ira N.; Hardin, John A.; Craft, Joe.

In: Arthritis and Rheumatism, Vol. 39, No. 1, 01.1996, p. 146-151.

Research output: Contribution to journalArticle

Hirakata, M, Suwa, A, Takeda, Y, Matsuoka, Y, Irimajiri, S, Targoff, IN, Hardin, JA & Craft, J 1996, 'Autoantibodies to glycyl-transfer RNA synthetase in myositis', Arthritis and Rheumatism, vol. 39, no. 1, pp. 146-151.
Hirakata M, Suwa A, Takeda Y, Matsuoka Y, Irimajiri S, Targoff IN et al. Autoantibodies to glycyl-transfer RNA synthetase in myositis. Arthritis and Rheumatism. 1996 Jan;39(1):146-151.
Hirakata, Michito ; Suwa, Akira ; Takeda, Yoshihiko ; Matsuoka, Yasuo ; Irimajiri, Shoichiro ; Targoff, Ira N. ; Hardin, John A. ; Craft, Joe. / Autoantibodies to glycyl-transfer RNA synthetase in myositis. In: Arthritis and Rheumatism. 1996 ; Vol. 39, No. 1. pp. 146-151.
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