Autoantibody response against a novel testicular antigen protein highly expressed in testis (PHET) in SSc patients

Hidekata Yasuoka, Masataka Kuwana

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Systemic sclerosis (SSc) is characterized by excessive fibrosis and autoantibody production. However, the pathogenesis of SSc is still under investigation. We have demonstrated that a novel testicular antigen, protein highly expressed in testis (PHET), is overexpressed in SSc dermal fibroblasts and targeted by autoantibodies. PHET was identified by screening of HepG2 cDNA library using an SSc serum and was found to belong to UniGene cluster of sperm associated antigen 9 (SPAG9) from its nucleotide sequence. PHET mRNA expression was examined by RT-PCR using mRNA panels of human tissues, and PHET mRNA was highly expressed only in testis in normal tissues. Anti-PHET antibodies were detected in 8.4% of sera of SSc patients by immunoblotting, and associated with diffuse scleroderma and lung involvement. Expression of PHET mRNA and protein was increased in cultured SSc dermal fibroblasts compared with control fibroblasts. These results suggest that ectopic expression of PHET in dermal fibroblasts induces autoantibody production against PHET in patients with SSc.

Original languageEnglish
Pages (from-to)228-231
Number of pages4
JournalAutoimmunity Reviews
Volume6
Issue number4
DOIs
Publication statusPublished - 2007 Mar
Externally publishedYes

Fingerprint

Systemic Scleroderma
Autoantibodies
Testis
Antigens
Proteins
Fibroblasts
Messenger RNA
Skin
Diffuse Scleroderma
Serum
Gene Library
Immunoblotting
Spermatozoa
Fibrosis
Polymerase Chain Reaction
Lung
Antibodies

Keywords

  • Autoantibody
  • Protein highly expressed in testis (PHET)
  • Systemic sclerosis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Autoantibody response against a novel testicular antigen protein highly expressed in testis (PHET) in SSc patients. / Yasuoka, Hidekata; Kuwana, Masataka.

In: Autoimmunity Reviews, Vol. 6, No. 4, 03.2007, p. 228-231.

Research output: Contribution to journalArticle

@article{09fc0f90efa04eef8e74b98cd49fcb72,
title = "Autoantibody response against a novel testicular antigen protein highly expressed in testis (PHET) in SSc patients",
abstract = "Systemic sclerosis (SSc) is characterized by excessive fibrosis and autoantibody production. However, the pathogenesis of SSc is still under investigation. We have demonstrated that a novel testicular antigen, protein highly expressed in testis (PHET), is overexpressed in SSc dermal fibroblasts and targeted by autoantibodies. PHET was identified by screening of HepG2 cDNA library using an SSc serum and was found to belong to UniGene cluster of sperm associated antigen 9 (SPAG9) from its nucleotide sequence. PHET mRNA expression was examined by RT-PCR using mRNA panels of human tissues, and PHET mRNA was highly expressed only in testis in normal tissues. Anti-PHET antibodies were detected in 8.4{\%} of sera of SSc patients by immunoblotting, and associated with diffuse scleroderma and lung involvement. Expression of PHET mRNA and protein was increased in cultured SSc dermal fibroblasts compared with control fibroblasts. These results suggest that ectopic expression of PHET in dermal fibroblasts induces autoantibody production against PHET in patients with SSc.",
keywords = "Autoantibody, Protein highly expressed in testis (PHET), Systemic sclerosis",
author = "Hidekata Yasuoka and Masataka Kuwana",
year = "2007",
month = "3",
doi = "10.1016/j.autrev.2006.08.006",
language = "English",
volume = "6",
pages = "228--231",
journal = "Autoimmunity Reviews",
issn = "1568-9972",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Autoantibody response against a novel testicular antigen protein highly expressed in testis (PHET) in SSc patients

AU - Yasuoka, Hidekata

AU - Kuwana, Masataka

PY - 2007/3

Y1 - 2007/3

N2 - Systemic sclerosis (SSc) is characterized by excessive fibrosis and autoantibody production. However, the pathogenesis of SSc is still under investigation. We have demonstrated that a novel testicular antigen, protein highly expressed in testis (PHET), is overexpressed in SSc dermal fibroblasts and targeted by autoantibodies. PHET was identified by screening of HepG2 cDNA library using an SSc serum and was found to belong to UniGene cluster of sperm associated antigen 9 (SPAG9) from its nucleotide sequence. PHET mRNA expression was examined by RT-PCR using mRNA panels of human tissues, and PHET mRNA was highly expressed only in testis in normal tissues. Anti-PHET antibodies were detected in 8.4% of sera of SSc patients by immunoblotting, and associated with diffuse scleroderma and lung involvement. Expression of PHET mRNA and protein was increased in cultured SSc dermal fibroblasts compared with control fibroblasts. These results suggest that ectopic expression of PHET in dermal fibroblasts induces autoantibody production against PHET in patients with SSc.

AB - Systemic sclerosis (SSc) is characterized by excessive fibrosis and autoantibody production. However, the pathogenesis of SSc is still under investigation. We have demonstrated that a novel testicular antigen, protein highly expressed in testis (PHET), is overexpressed in SSc dermal fibroblasts and targeted by autoantibodies. PHET was identified by screening of HepG2 cDNA library using an SSc serum and was found to belong to UniGene cluster of sperm associated antigen 9 (SPAG9) from its nucleotide sequence. PHET mRNA expression was examined by RT-PCR using mRNA panels of human tissues, and PHET mRNA was highly expressed only in testis in normal tissues. Anti-PHET antibodies were detected in 8.4% of sera of SSc patients by immunoblotting, and associated with diffuse scleroderma and lung involvement. Expression of PHET mRNA and protein was increased in cultured SSc dermal fibroblasts compared with control fibroblasts. These results suggest that ectopic expression of PHET in dermal fibroblasts induces autoantibody production against PHET in patients with SSc.

KW - Autoantibody

KW - Protein highly expressed in testis (PHET)

KW - Systemic sclerosis

UR - http://www.scopus.com/inward/record.url?scp=33847027591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847027591&partnerID=8YFLogxK

U2 - 10.1016/j.autrev.2006.08.006

DO - 10.1016/j.autrev.2006.08.006

M3 - Article

C2 - 17317613

AN - SCOPUS:33847027591

VL - 6

SP - 228

EP - 231

JO - Autoimmunity Reviews

JF - Autoimmunity Reviews

SN - 1568-9972

IS - 4

ER -