Autoimmune regulator is expressed in the cells regulating immune tolerance in thymus medulla

Maarit Heino, Pärt Peterson, Jun Kudo, Kentaro Nagamine, Anssi Lagerstedt, Vladimir Ovod, Annamari Ranki, Immo Rantala, Markku Nieminen, Juha Tuukkanen, Hamish S. Scott, Stylianos E. Antonarakis, Nobuyoshi Shimizu, Kai Krohn

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

The AIRE gene (autoimmune regulator), coding for a putative transcriptional regulatory factor, is mutated in autoimmune-polyendocrinopathy-candidiasis ectodermal dystrophy (APECED). We have investigated the expression of the AIRE gene by mRNA in situ hybridization and immunohistochemistry in various human tissues. Here we show that AIRE is expressed in distinct cells in thymus medulla, and also in rare cells in lymph node paracortex and medulla, and in spleen and fetal liver, but not in the target organs of autoimmune destruction. Double immunofluorescence studies revealed that in thymus medulla both epithelial (cytokeratin positive) and non-epithelial cells expressed AIRE. Subcellularly, AIRE was localised in nuclear dots in thymus and lymph node and also in transfected cells. The cellular localisation of AIRE and its nuclear localisation, compatible with its predicted protein domains, suggest that AlRE may regulate the mechanisms involved in the induction and maintenance of immune tolerance.

Original languageEnglish
Pages (from-to)821-825
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume257
Issue number3
DOIs
Publication statusPublished - 1999 Apr 21
Externally publishedYes

Fingerprint

Thymus
Immune Tolerance
Thymus Gland
Genes
Lymph Nodes
Autoimmune Polyendocrinopathies
Keratins
Liver
Regulator Genes
Cells
Tissue
In Situ Hybridization
Fluorescent Antibody Technique
Messenger RNA
Spleen
Immunohistochemistry
Maintenance
Gene Expression
Proteins

Keywords

  • Autoimmunity
  • Monocyte-dendritic cell
  • Polyendocrinopathy
  • Thymic epithelial cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Autoimmune regulator is expressed in the cells regulating immune tolerance in thymus medulla. / Heino, Maarit; Peterson, Pärt; Kudo, Jun; Nagamine, Kentaro; Lagerstedt, Anssi; Ovod, Vladimir; Ranki, Annamari; Rantala, Immo; Nieminen, Markku; Tuukkanen, Juha; Scott, Hamish S.; Antonarakis, Stylianos E.; Shimizu, Nobuyoshi; Krohn, Kai.

In: Biochemical and Biophysical Research Communications, Vol. 257, No. 3, 21.04.1999, p. 821-825.

Research output: Contribution to journalArticle

Heino, M, Peterson, P, Kudo, J, Nagamine, K, Lagerstedt, A, Ovod, V, Ranki, A, Rantala, I, Nieminen, M, Tuukkanen, J, Scott, HS, Antonarakis, SE, Shimizu, N & Krohn, K 1999, 'Autoimmune regulator is expressed in the cells regulating immune tolerance in thymus medulla', Biochemical and Biophysical Research Communications, vol. 257, no. 3, pp. 821-825. https://doi.org/10.1006/bbrc.1999.0308
Heino, Maarit ; Peterson, Pärt ; Kudo, Jun ; Nagamine, Kentaro ; Lagerstedt, Anssi ; Ovod, Vladimir ; Ranki, Annamari ; Rantala, Immo ; Nieminen, Markku ; Tuukkanen, Juha ; Scott, Hamish S. ; Antonarakis, Stylianos E. ; Shimizu, Nobuyoshi ; Krohn, Kai. / Autoimmune regulator is expressed in the cells regulating immune tolerance in thymus medulla. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 257, No. 3. pp. 821-825.
@article{d74bff5f29104802aed50ab05344cad7,
title = "Autoimmune regulator is expressed in the cells regulating immune tolerance in thymus medulla",
abstract = "The AIRE gene (autoimmune regulator), coding for a putative transcriptional regulatory factor, is mutated in autoimmune-polyendocrinopathy-candidiasis ectodermal dystrophy (APECED). We have investigated the expression of the AIRE gene by mRNA in situ hybridization and immunohistochemistry in various human tissues. Here we show that AIRE is expressed in distinct cells in thymus medulla, and also in rare cells in lymph node paracortex and medulla, and in spleen and fetal liver, but not in the target organs of autoimmune destruction. Double immunofluorescence studies revealed that in thymus medulla both epithelial (cytokeratin positive) and non-epithelial cells expressed AIRE. Subcellularly, AIRE was localised in nuclear dots in thymus and lymph node and also in transfected cells. The cellular localisation of AIRE and its nuclear localisation, compatible with its predicted protein domains, suggest that AlRE may regulate the mechanisms involved in the induction and maintenance of immune tolerance.",
keywords = "Autoimmunity, Monocyte-dendritic cell, Polyendocrinopathy, Thymic epithelial cell",
author = "Maarit Heino and P{\"a}rt Peterson and Jun Kudo and Kentaro Nagamine and Anssi Lagerstedt and Vladimir Ovod and Annamari Ranki and Immo Rantala and Markku Nieminen and Juha Tuukkanen and Scott, {Hamish S.} and Antonarakis, {Stylianos E.} and Nobuyoshi Shimizu and Kai Krohn",
year = "1999",
month = "4",
day = "21",
doi = "10.1006/bbrc.1999.0308",
language = "English",
volume = "257",
pages = "821--825",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Autoimmune regulator is expressed in the cells regulating immune tolerance in thymus medulla

AU - Heino, Maarit

AU - Peterson, Pärt

AU - Kudo, Jun

AU - Nagamine, Kentaro

AU - Lagerstedt, Anssi

AU - Ovod, Vladimir

AU - Ranki, Annamari

AU - Rantala, Immo

AU - Nieminen, Markku

AU - Tuukkanen, Juha

AU - Scott, Hamish S.

AU - Antonarakis, Stylianos E.

AU - Shimizu, Nobuyoshi

AU - Krohn, Kai

PY - 1999/4/21

Y1 - 1999/4/21

N2 - The AIRE gene (autoimmune regulator), coding for a putative transcriptional regulatory factor, is mutated in autoimmune-polyendocrinopathy-candidiasis ectodermal dystrophy (APECED). We have investigated the expression of the AIRE gene by mRNA in situ hybridization and immunohistochemistry in various human tissues. Here we show that AIRE is expressed in distinct cells in thymus medulla, and also in rare cells in lymph node paracortex and medulla, and in spleen and fetal liver, but not in the target organs of autoimmune destruction. Double immunofluorescence studies revealed that in thymus medulla both epithelial (cytokeratin positive) and non-epithelial cells expressed AIRE. Subcellularly, AIRE was localised in nuclear dots in thymus and lymph node and also in transfected cells. The cellular localisation of AIRE and its nuclear localisation, compatible with its predicted protein domains, suggest that AlRE may regulate the mechanisms involved in the induction and maintenance of immune tolerance.

AB - The AIRE gene (autoimmune regulator), coding for a putative transcriptional regulatory factor, is mutated in autoimmune-polyendocrinopathy-candidiasis ectodermal dystrophy (APECED). We have investigated the expression of the AIRE gene by mRNA in situ hybridization and immunohistochemistry in various human tissues. Here we show that AIRE is expressed in distinct cells in thymus medulla, and also in rare cells in lymph node paracortex and medulla, and in spleen and fetal liver, but not in the target organs of autoimmune destruction. Double immunofluorescence studies revealed that in thymus medulla both epithelial (cytokeratin positive) and non-epithelial cells expressed AIRE. Subcellularly, AIRE was localised in nuclear dots in thymus and lymph node and also in transfected cells. The cellular localisation of AIRE and its nuclear localisation, compatible with its predicted protein domains, suggest that AlRE may regulate the mechanisms involved in the induction and maintenance of immune tolerance.

KW - Autoimmunity

KW - Monocyte-dendritic cell

KW - Polyendocrinopathy

KW - Thymic epithelial cell

UR - http://www.scopus.com/inward/record.url?scp=0033590981&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033590981&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1999.0308

DO - 10.1006/bbrc.1999.0308

M3 - Article

C2 - 10208866

AN - SCOPUS:0033590981

VL - 257

SP - 821

EP - 825

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -