Autoimmune regulator is expressed in the cells regulating immune tolerance in thymus medulla

Maarit Heino, Pärt Peterson, Jun Kudoh, Kentaro Nagamine, Anssi Lagerstedt, Vladimir Ovod, Annamari Ranki, Immo Rantala, Markku Nieminen, Juha Tuukkanen, Hamish S. Scott, Stylianos E. Antonarakis, Nobuyoshi Shimizu, Kai Krohn

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

The AIRE gene (autoimmune regulator), coding for a putative transcriptional regulatory factor, is mutated in autoimmune-polyendocrinopathy-candidiasis ectodermal dystrophy (APECED). We have investigated the expression of the AIRE gene by mRNA in situ hybridization and immunohistochemistry in various human tissues. Here we show that AIRE is expressed in distinct cells in thymus medulla, and also in rare cells in lymph node paracortex and medulla, and in spleen and fetal liver, but not in the target organs of autoimmune destruction. Double immunofluorescence studies revealed that in thymus medulla both epithelial (cytokeratin positive) and non-epithelial cells expressed AIRE. Subcellularly, AIRE was localised in nuclear dots in thymus and lymph node and also in transfected cells. The cellular localisation of AIRE and its nuclear localisation, compatible with its predicted protein domains, suggest that AlRE may regulate the mechanisms involved in the induction and maintenance of immune tolerance.

Original languageEnglish
Pages (from-to)821-825
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume257
Issue number3
DOIs
Publication statusPublished - 1999 Apr 21

Keywords

  • Autoimmunity
  • Monocyte-dendritic cell
  • Polyendocrinopathy
  • Thymic epithelial cell

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Heino, M., Peterson, P., Kudoh, J., Nagamine, K., Lagerstedt, A., Ovod, V., Ranki, A., Rantala, I., Nieminen, M., Tuukkanen, J., Scott, H. S., Antonarakis, S. E., Shimizu, N., & Krohn, K. (1999). Autoimmune regulator is expressed in the cells regulating immune tolerance in thymus medulla. Biochemical and Biophysical Research Communications, 257(3), 821-825. https://doi.org/10.1006/bbrc.1999.0308