Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis

Shigeaki Suzuki; Kimiaki Utsugisawa; Kazuo Iwasa; Takashi Satoh; Yuriko Nagane; Hiroaki Yoshikawa; Masataka Kuwana; Norihiro Suzuki

doi:10.1016/j.jneuroim.2011.06.011

Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis

Shigeaki Suzuki, Kimiaki Utsugisawa, Kazuo Iwasa, Takashi Satoh, Yuriko Nagane, Hiroaki Yoshikawa, Masataka Kuwana, Norihiro Suzuki

Department of Internal Medicine (Neurology)

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Immune responses to ER stress have been closely related to the pathogenesis of autoimmune diseases. Using an immunoprecipitation assay, 24 (7.1%) of 336 MG serum samples immunoprecipitated a 90-kDa protein from the muscle cellular extracts, but none of the disease or healthy control sera. The 90-kDa protein was affinity-purified and found to match to ER chaperon GRP94 by matrix-assisted laser desorption/ionization-time of flight mass spectroscopy analysis. The frequency of associated autoimmune diseases was much higher in the anti-GRP94-positive than the -negative patients (71% versus 11%, p < 0.001). Autoimmunity to ER chaperone GRP94 is associated with a subset of MG patients who have additional autoimmune diseases.

Original language	English
Pages (from-to)	87-92
Number of pages	6
Journal	Journal of Neuroimmunology
Volume	237
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2011.06.011
Publication status	Published - 2011 Aug 15

Keywords

Autoantibody
Autoimmune diseases
ER stress
GRP94
Immunoprecipitation
Myasthenia gravis

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2011.06.011

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title = "Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis",

abstract = "Immune responses to ER stress have been closely related to the pathogenesis of autoimmune diseases. Using an immunoprecipitation assay, 24 (7.1%) of 336 MG serum samples immunoprecipitated a 90-kDa protein from the muscle cellular extracts, but none of the disease or healthy control sera. The 90-kDa protein was affinity-purified and found to match to ER chaperon GRP94 by matrix-assisted laser desorption/ionization-time of flight mass spectroscopy analysis. The frequency of associated autoimmune diseases was much higher in the anti-GRP94-positive than the -negative patients (71% versus 11%, p < 0.001). Autoimmunity to ER chaperone GRP94 is associated with a subset of MG patients who have additional autoimmune diseases.",

keywords = "Autoantibody, Autoimmune diseases, ER stress, GRP94, Immunoprecipitation, Myasthenia gravis",

author = "Shigeaki Suzuki and Kimiaki Utsugisawa and Kazuo Iwasa and Takashi Satoh and Yuriko Nagane and Hiroaki Yoshikawa and Masataka Kuwana and Norihiro Suzuki",

note = "Funding Information: This work was supported by a grant from the Japanese Ministry of Education, Science, Sports and Culture (no. 23591255 ) and a Neuroimmunological Disease Research Committee grant from the Japanese Ministry of Health, Labour, and Welfare .",

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AU - Suzuki, Shigeaki

AU - Utsugisawa, Kimiaki

AU - Iwasa, Kazuo

AU - Satoh, Takashi

AU - Nagane, Yuriko

AU - Yoshikawa, Hiroaki

AU - Kuwana, Masataka

AU - Suzuki, Norihiro

N1 - Funding Information: This work was supported by a grant from the Japanese Ministry of Education, Science, Sports and Culture (no. 23591255 ) and a Neuroimmunological Disease Research Committee grant from the Japanese Ministry of Health, Labour, and Welfare .

PY - 2011/8/15

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N2 - Immune responses to ER stress have been closely related to the pathogenesis of autoimmune diseases. Using an immunoprecipitation assay, 24 (7.1%) of 336 MG serum samples immunoprecipitated a 90-kDa protein from the muscle cellular extracts, but none of the disease or healthy control sera. The 90-kDa protein was affinity-purified and found to match to ER chaperon GRP94 by matrix-assisted laser desorption/ionization-time of flight mass spectroscopy analysis. The frequency of associated autoimmune diseases was much higher in the anti-GRP94-positive than the -negative patients (71% versus 11%, p < 0.001). Autoimmunity to ER chaperone GRP94 is associated with a subset of MG patients who have additional autoimmune diseases.

AB - Immune responses to ER stress have been closely related to the pathogenesis of autoimmune diseases. Using an immunoprecipitation assay, 24 (7.1%) of 336 MG serum samples immunoprecipitated a 90-kDa protein from the muscle cellular extracts, but none of the disease or healthy control sera. The 90-kDa protein was affinity-purified and found to match to ER chaperon GRP94 by matrix-assisted laser desorption/ionization-time of flight mass spectroscopy analysis. The frequency of associated autoimmune diseases was much higher in the anti-GRP94-positive than the -negative patients (71% versus 11%, p < 0.001). Autoimmunity to ER chaperone GRP94 is associated with a subset of MG patients who have additional autoimmune diseases.

KW - Autoantibody

KW - Autoimmune diseases

KW - ER stress

KW - GRP94

KW - Immunoprecipitation

KW - Myasthenia gravis

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Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis

Abstract

Keywords

ASJC Scopus subject areas

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