Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis

Shigeaki Suzuki, Kimiaki Utsugisawa, Kazuo Iwasa, Takashi Satoh, Yuriko Nagane, Hiroaki Yoshikawa, Masataka Kuwana, Norihiro Suzuki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Immune responses to ER stress have been closely related to the pathogenesis of autoimmune diseases. Using an immunoprecipitation assay, 24 (7.1%) of 336 MG serum samples immunoprecipitated a 90-kDa protein from the muscle cellular extracts, but none of the disease or healthy control sera. The 90-kDa protein was affinity-purified and found to match to ER chaperon GRP94 by matrix-assisted laser desorption/ionization-time of flight mass spectroscopy analysis. The frequency of associated autoimmune diseases was much higher in the anti-GRP94-positive than the -negative patients (71% versus 11%, p < 0.001). Autoimmunity to ER chaperone GRP94 is associated with a subset of MG patients who have additional autoimmune diseases.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalJournal of Neuroimmunology
Volume237
Issue number1-2
DOIs
Publication statusPublished - 2011 Aug 15

Fingerprint

Myasthenia Gravis
Autoimmunity
Endoplasmic Reticulum
Autoimmune Diseases
Muscle Proteins
Serum
Immunoprecipitation
Mass Spectrometry
Lasers
glucose-regulated proteins
Proteins

Keywords

  • Autoantibody
  • Autoimmune diseases
  • ER stress
  • GRP94
  • Immunoprecipitation
  • Myasthenia gravis

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Suzuki, S., Utsugisawa, K., Iwasa, K., Satoh, T., Nagane, Y., Yoshikawa, H., ... Suzuki, N. (2011). Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis. Journal of Neuroimmunology, 237(1-2), 87-92. https://doi.org/10.1016/j.jneuroim.2011.06.011

Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis. / Suzuki, Shigeaki; Utsugisawa, Kimiaki; Iwasa, Kazuo; Satoh, Takashi; Nagane, Yuriko; Yoshikawa, Hiroaki; Kuwana, Masataka; Suzuki, Norihiro.

In: Journal of Neuroimmunology, Vol. 237, No. 1-2, 15.08.2011, p. 87-92.

Research output: Contribution to journalArticle

Suzuki, S, Utsugisawa, K, Iwasa, K, Satoh, T, Nagane, Y, Yoshikawa, H, Kuwana, M & Suzuki, N 2011, 'Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis', Journal of Neuroimmunology, vol. 237, no. 1-2, pp. 87-92. https://doi.org/10.1016/j.jneuroim.2011.06.011
Suzuki, Shigeaki ; Utsugisawa, Kimiaki ; Iwasa, Kazuo ; Satoh, Takashi ; Nagane, Yuriko ; Yoshikawa, Hiroaki ; Kuwana, Masataka ; Suzuki, Norihiro. / Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis. In: Journal of Neuroimmunology. 2011 ; Vol. 237, No. 1-2. pp. 87-92.
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