Automated determination of carboxyhemoglobin contents in autopsy materials using head-space gas chromatography/mass spectrometry

Shigeki Oritani, Bao Li Zhu, Kaori Ishida, Keiko Shimotouge, Li Quan, Masaki Q. Fujita, Hitoshi Maeda

Research output: Contribution to journalArticle

34 Citations (Scopus)


To establish a method for the routine analysis of carboxyhemoglobin (COHb) in autopsy materials including those which have undergone postmortem changes, e.g. thermo-coagulation, putrifaction and contamination, an automated head-space gas chromatography/mass spectrometry (GC/MS) analysis was utilized. The procedure consisted of preparation of the sample in a vial and a carbon monoxide (CO) saturated sample, for estimation of hemoglobin content, in another vial, the addition of n-octanol, potassium ferricyanide and an internal standard (t-butanol), GC separation and determination of CO using a GC/MS system equipped with an automated head-space gas sampler. The method was practical not only with the blood and bone marrow aspirates to confirm the findings on the CO-oximeter system, but also with the thermo-coagulated and putrified blood. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)375-379
Number of pages5
JournalForensic Science International
Issue number1-3
Publication statusPublished - 2000 Sep 11



  • Bone marrow
  • Carboxyhemoglobin
  • Forensic toxicology
  • Gas chromatography/mass spectrometry
  • Thermocoagulated blood

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this