Automated determination of carboxyhemoglobin contents in autopsy materials using head-space gas chromatography/mass spectrometry

Shigeki Oritani, Bao Li Zhu, Kaori Ishida, Keiko Shimotouge, Li Quan, Masaki Q Fujita, Hitoshi Maeda

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

To establish a method for the routine analysis of carboxyhemoglobin (COHb) in autopsy materials including those which have undergone postmortem changes, e.g. thermo-coagulation, putrifaction and contamination, an automated head-space gas chromatography/mass spectrometry (GC/MS) analysis was utilized. The procedure consisted of preparation of the sample in a vial and a carbon monoxide (CO) saturated sample, for estimation of hemoglobin content, in another vial, the addition of n-octanol, potassium ferricyanide and an internal standard (t-butanol), GC separation and determination of CO using a GC/MS system equipped with an automated head-space gas sampler. The method was practical not only with the blood and bone marrow aspirates to confirm the findings on the CO-oximeter system, but also with the thermo-coagulated and putrified blood. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)375-379
Number of pages5
JournalForensic Science International
Volume113
Issue number1-3
DOIs
Publication statusPublished - 2000 Sep 11
Externally publishedYes

Fingerprint

Carboxyhemoglobin
Carbon Monoxide
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Autopsy
Head
Blood
Postmortem Changes
tert-Butyl Alcohol
Oximeters
1-Octanol
Coagulation
Bone
Hemoglobins
Contamination
Gases
Bone Marrow

Keywords

  • Bone marrow
  • Carboxyhemoglobin
  • Forensic toxicology
  • Gas chromatography/mass spectrometry
  • Thermocoagulated blood

ASJC Scopus subject areas

  • Genetics
  • Analytical Chemistry
  • Pathology and Forensic Medicine
  • Psychiatry and Mental health

Cite this

Automated determination of carboxyhemoglobin contents in autopsy materials using head-space gas chromatography/mass spectrometry. / Oritani, Shigeki; Zhu, Bao Li; Ishida, Kaori; Shimotouge, Keiko; Quan, Li; Fujita, Masaki Q; Maeda, Hitoshi.

In: Forensic Science International, Vol. 113, No. 1-3, 11.09.2000, p. 375-379.

Research output: Contribution to journalArticle

Oritani, Shigeki ; Zhu, Bao Li ; Ishida, Kaori ; Shimotouge, Keiko ; Quan, Li ; Fujita, Masaki Q ; Maeda, Hitoshi. / Automated determination of carboxyhemoglobin contents in autopsy materials using head-space gas chromatography/mass spectrometry. In: Forensic Science International. 2000 ; Vol. 113, No. 1-3. pp. 375-379.
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