Automatic analysis for neuron by confocal laser scanning microscope

Kouhei Satou, Yoshimitsu Aoki, Nobuko Mataga, Takao K. Hensh, Katuhiko Taki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The aim of this study is to develop a system that recognizes both the macro- and microscopic configurations of nerve cells and automatically performs the necessary 3-D measurements and functional classification of spines. The acquisition of 3-D images of cranial nerves has been enabled by the use of a confocal laser scanning microscope, although the highly accurate 3-D measurements of the microscopic structures of cranial nerves and their classification based on their configurations have not yet been accomplished. In this study, in order to obtain highly accurate measurements of the microscopic structures of cranial nerves, existing positions of spines were predicted by the 2-D image processing of tomographic images. Next, based on the positions that were predicted on the 2-D images, the positions and configurations of the spines were determined more accurately by 3-D image processing of the volume data. We report the successful construction of an automatic analysis system that uses a coarse-to-fine technique to analyze the microscopic structures of cranial nerves with high speed and accuracy by combining 2-D and 3-D image analyses.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsK. Sumi
Volume6051
DOIs
Publication statusPublished - 2005
Externally publishedYes
EventOptomechatronic Machine Vision - Sappora, Japan
Duration: 2005 Dec 52005 Dec 7

Other

OtherOptomechatronic Machine Vision
CountryJapan
CitySappora
Period05/12/505/12/7

Fingerprint

nerves
neurons
Neurons
Microscopes
microscopes
spine
Scanning
scanning
Lasers
Image processing
lasers
image processing
configurations
Macros
systems analysis
acquisition
high speed
cells

Keywords

  • Confocal laser scanning microscope
  • Neuron image analysis
  • Pattern recognition
  • Spine

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Satou, K., Aoki, Y., Mataga, N., Hensh, T. K., & Taki, K. (2005). Automatic analysis for neuron by confocal laser scanning microscope. In K. Sumi (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 6051). [60510A] https://doi.org/10.1117/12.649449

Automatic analysis for neuron by confocal laser scanning microscope. / Satou, Kouhei; Aoki, Yoshimitsu; Mataga, Nobuko; Hensh, Takao K.; Taki, Katuhiko.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / K. Sumi. Vol. 6051 2005. 60510A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Satou, K, Aoki, Y, Mataga, N, Hensh, TK & Taki, K 2005, Automatic analysis for neuron by confocal laser scanning microscope. in K Sumi (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 6051, 60510A, Optomechatronic Machine Vision, Sappora, Japan, 05/12/5. https://doi.org/10.1117/12.649449
Satou K, Aoki Y, Mataga N, Hensh TK, Taki K. Automatic analysis for neuron by confocal laser scanning microscope. In Sumi K, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6051. 2005. 60510A https://doi.org/10.1117/12.649449
Satou, Kouhei ; Aoki, Yoshimitsu ; Mataga, Nobuko ; Hensh, Takao K. ; Taki, Katuhiko. / Automatic analysis for neuron by confocal laser scanning microscope. Proceedings of SPIE - The International Society for Optical Engineering. editor / K. Sumi. Vol. 6051 2005.
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