Automatic generation system of cell-sized liposomes

Masahide Gotanda, Koki Kamiya, Toshihisa Osaki, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Artificial cell membranes such as liposomes and lipid vesicles have been developed using microfluidic technologies. These technologies have advantages for generating cell-sized liposomes, including a monodispersed and asymmetric lipid composition. Cell-sized asymmetric liposomes with various combinations of asymmetric lipid leaflets are sequentially produced using an improved pulsed-jetting device with the six wells arranged on a circular rotation table by manual manipulation. In this study, we reveal optimal parameters of rotation speed and jetting timing for the cell-sized liposome formation using an automatic sequential liposome generation system driven by a stepping motor. First, we optimize the rotation speed of the stepping motor for forming the planar lipid bilayer under control of a microcomputer. Next, we generate cell-sized liposomes using this automated system at the optimized rotation speed. This automatic system will contribute to high-throughput investigations of membrane protein activities and functions using the electrophysiological or the asymmetric liposome analyses.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume292
DOIs
Publication statusPublished - 2019 Aug 1

Keywords

  • Artificial cell membrane
  • Asymmetric lipid vesicle
  • Automated lipid bilayer formation
  • Lipid-protein interaction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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