Automatic generation system of cell-sized liposomes

Masahide Gotanda, Koki Kamiya, Toshihisa Osaki, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticle

Abstract

Artificial cell membranes such as liposomes and lipid vesicles have been developed using microfluidic technologies. These technologies have advantages for generating cell-sized liposomes, including a monodispersed and asymmetric lipid composition. Cell-sized asymmetric liposomes with various combinations of asymmetric lipid leaflets are sequentially produced using an improved pulsed-jetting device with the six wells arranged on a circular rotation table by manual manipulation. In this study, we reveal optimal parameters of rotation speed and jetting timing for the cell-sized liposome formation using an automatic sequential liposome generation system driven by a stepping motor. First, we optimize the rotation speed of the stepping motor for forming the planar lipid bilayer under control of a microcomputer. Next, we generate cell-sized liposomes using this automated system at the optimized rotation speed. This automatic system will contribute to high-throughput investigations of membrane protein activities and functions using the electrophysiological or the asymmetric liposome analyses.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume292
DOIs
Publication statusPublished - 2019 Aug 1

Fingerprint

Liposomes
lipids
stepping motors
cells
Lipids
Stepping motors
microcomputers
manipulators
time measurement
Lipid bilayers
membranes
proteins
Cell membranes
Microfluidics
Microcomputers
Membrane Proteins
Throughput
Proteins
Membranes
Chemical analysis

Keywords

  • Artificial cell membrane
  • Asymmetric lipid vesicle
  • Automated lipid bilayer formation
  • Lipid-protein interaction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Automatic generation system of cell-sized liposomes. / Gotanda, Masahide; Kamiya, Koki; Osaki, Toshihisa; Miki, Norihisa; Takeuchi, Shoji.

In: Sensors and Actuators, B: Chemical, Vol. 292, 01.08.2019, p. 57-63.

Research output: Contribution to journalArticle

Gotanda, Masahide ; Kamiya, Koki ; Osaki, Toshihisa ; Miki, Norihisa ; Takeuchi, Shoji. / Automatic generation system of cell-sized liposomes. In: Sensors and Actuators, B: Chemical. 2019 ; Vol. 292. pp. 57-63.
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