Autophosphorylation of a Newly Identified Site of Aurora-B Is Indispensable for Cytokinesis

Yoshihiro Yasui, Takeshi Urano, Aie Kawajiri, Koh Ichi Nagata, Masaaki Tatsuka, Hideyuki Saya, Koichi Furukawa, Toshitada Takahashi, Ichiro Izawa, Masaki Inagaki

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Mitotic kinases regulate cell division and its checkpoints, errors of which can lead to aneuploidy or genetic instability. One of these is Aurora-B, a key kinase that is required for chromosome alignment at the metaphase plate and for cytokinesis in mammalian cells. We report here that human Aurora-B is phosphorylated at Thr-232 through interaction with the inner centromere protein (INCENP) in vivo. The phosphorylation of Thr-232 occurs by means of an autophosphorylation mechanism, which is indispensable for the Aurora-B kinase activity. The activation of Aurora-B spatio-temporally correlated with the site-specific phosphorylation of its physiological substrates, histone H3 and vimentin. Overexpression of the TA mutant of Aurora-B, in which Thr-232 was changed into alanine, frequently induced multinuclearity in cells. These results indicate that the phosphorylation of Thr-232 is an essential regulatory mechanism for Aurora-B activation.

Original languageEnglish
Pages (from-to)12997-13003
Number of pages7
JournalJournal of Biological Chemistry
Volume279
Issue number13
DOIs
Publication statusPublished - 2004 Mar 26
Externally publishedYes

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Phosphorylation
Cytokinesis
Phosphotransferases
Aurora Kinase B
Chemical activation
Cells
Centromere
Aneuploidy
Vimentin
Metaphase
Chromosomes
Alanine
Cell Division
Histones
Substrates
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yasui, Y., Urano, T., Kawajiri, A., Nagata, K. I., Tatsuka, M., Saya, H., ... Inagaki, M. (2004). Autophosphorylation of a Newly Identified Site of Aurora-B Is Indispensable for Cytokinesis. Journal of Biological Chemistry, 279(13), 12997-13003. https://doi.org/10.1074/jbc.M311128200

Autophosphorylation of a Newly Identified Site of Aurora-B Is Indispensable for Cytokinesis. / Yasui, Yoshihiro; Urano, Takeshi; Kawajiri, Aie; Nagata, Koh Ichi; Tatsuka, Masaaki; Saya, Hideyuki; Furukawa, Koichi; Takahashi, Toshitada; Izawa, Ichiro; Inagaki, Masaki.

In: Journal of Biological Chemistry, Vol. 279, No. 13, 26.03.2004, p. 12997-13003.

Research output: Contribution to journalArticle

Yasui, Y, Urano, T, Kawajiri, A, Nagata, KI, Tatsuka, M, Saya, H, Furukawa, K, Takahashi, T, Izawa, I & Inagaki, M 2004, 'Autophosphorylation of a Newly Identified Site of Aurora-B Is Indispensable for Cytokinesis', Journal of Biological Chemistry, vol. 279, no. 13, pp. 12997-13003. https://doi.org/10.1074/jbc.M311128200
Yasui, Yoshihiro ; Urano, Takeshi ; Kawajiri, Aie ; Nagata, Koh Ichi ; Tatsuka, Masaaki ; Saya, Hideyuki ; Furukawa, Koichi ; Takahashi, Toshitada ; Izawa, Ichiro ; Inagaki, Masaki. / Autophosphorylation of a Newly Identified Site of Aurora-B Is Indispensable for Cytokinesis. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 13. pp. 12997-13003.
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