Autoradiographic visualization of a calcium channel antagonist, [125I]ω-conotoxin GVIA, binding site in the brains of normal and cerebellar mutant mice (pcd and weaver)

Nobuaki Maeda, Kentaro Wada, Michisuke Yuzaki, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

An in vitro autoradiographic technique has been used to localize [125I]ω-conotoxin GVIA binding sites in the brains of normal and cerebellar mutant mice. In the brains of normal mice, the highest densities of binding sites were observed at glomeruli of the olfactory bulb, cerebral cortex, caudate nucleus-putamen, hippocampus, and the nucleus of the solitary tract. Moderate densities of the silver grains occured on the granular layer of the olfactory bulb, the molecular layer of the dentate gyrus, the molecular layer of the cerebellum, and the cochlear nucleus. No specific binding appeared in the white matter or the deep nucleus of the cerebellum, the corpus callosum, the internal capsule and the external plexiform layer of the olfactory bulb. Autoradiographic studies of the cerebella of Purkinje cell degeneration (pcd) mice showed that the distribution of binding sites on the molecular layer of the cerebellum are not affected by the degeneration of Purkinje cells. However, only background levels of the silver grains occured on the cerebella of agranular weaver mutant mice, suggesting that the receptor for ω-conotoxin GVIA in the cerebellum are predominantly distributed on the parellel fibers of granule cells.

Original languageEnglish
Pages (from-to)21-30
Number of pages10
JournalBrain Research
Volume489
Issue number1
DOIs
Publication statusPublished - 1989 Jun 5
Externally publishedYes

Fingerprint

Conotoxins
Purkinje Cells
Calcium Channel Blockers
Cerebellum
Binding Sites
Olfactory Bulb
Brain
Silver
Neurologic Mutant Mice
Cochlear Nucleus
Internal Capsule
Solitary Nucleus
Corpus Callosum
Caudate Nucleus
Putamen
Dentate Gyrus
Cerebral Cortex
Hippocampus

Keywords

  • Autoradiography
  • Calcium channel
  • Cerebellar mutant mouse
  • ω-Conotoxin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Autoradiographic visualization of a calcium channel antagonist, [125I]ω-conotoxin GVIA, binding site in the brains of normal and cerebellar mutant mice (pcd and weaver). / Maeda, Nobuaki; Wada, Kentaro; Yuzaki, Michisuke; Mikoshiba, Katsuhiko.

In: Brain Research, Vol. 489, No. 1, 05.06.1989, p. 21-30.

Research output: Contribution to journalArticle

@article{aea98f99a3414b99bec17e4ca3f87525,
title = "Autoradiographic visualization of a calcium channel antagonist, [125I]ω-conotoxin GVIA, binding site in the brains of normal and cerebellar mutant mice (pcd and weaver)",
abstract = "An in vitro autoradiographic technique has been used to localize [125I]ω-conotoxin GVIA binding sites in the brains of normal and cerebellar mutant mice. In the brains of normal mice, the highest densities of binding sites were observed at glomeruli of the olfactory bulb, cerebral cortex, caudate nucleus-putamen, hippocampus, and the nucleus of the solitary tract. Moderate densities of the silver grains occured on the granular layer of the olfactory bulb, the molecular layer of the dentate gyrus, the molecular layer of the cerebellum, and the cochlear nucleus. No specific binding appeared in the white matter or the deep nucleus of the cerebellum, the corpus callosum, the internal capsule and the external plexiform layer of the olfactory bulb. Autoradiographic studies of the cerebella of Purkinje cell degeneration (pcd) mice showed that the distribution of binding sites on the molecular layer of the cerebellum are not affected by the degeneration of Purkinje cells. However, only background levels of the silver grains occured on the cerebella of agranular weaver mutant mice, suggesting that the receptor for ω-conotoxin GVIA in the cerebellum are predominantly distributed on the parellel fibers of granule cells.",
keywords = "Autoradiography, Calcium channel, Cerebellar mutant mouse, ω-Conotoxin",
author = "Nobuaki Maeda and Kentaro Wada and Michisuke Yuzaki and Katsuhiko Mikoshiba",
year = "1989",
month = "6",
day = "5",
doi = "10.1016/0006-8993(89)90004-8",
language = "English",
volume = "489",
pages = "21--30",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Autoradiographic visualization of a calcium channel antagonist, [125I]ω-conotoxin GVIA, binding site in the brains of normal and cerebellar mutant mice (pcd and weaver)

AU - Maeda, Nobuaki

AU - Wada, Kentaro

AU - Yuzaki, Michisuke

AU - Mikoshiba, Katsuhiko

PY - 1989/6/5

Y1 - 1989/6/5

N2 - An in vitro autoradiographic technique has been used to localize [125I]ω-conotoxin GVIA binding sites in the brains of normal and cerebellar mutant mice. In the brains of normal mice, the highest densities of binding sites were observed at glomeruli of the olfactory bulb, cerebral cortex, caudate nucleus-putamen, hippocampus, and the nucleus of the solitary tract. Moderate densities of the silver grains occured on the granular layer of the olfactory bulb, the molecular layer of the dentate gyrus, the molecular layer of the cerebellum, and the cochlear nucleus. No specific binding appeared in the white matter or the deep nucleus of the cerebellum, the corpus callosum, the internal capsule and the external plexiform layer of the olfactory bulb. Autoradiographic studies of the cerebella of Purkinje cell degeneration (pcd) mice showed that the distribution of binding sites on the molecular layer of the cerebellum are not affected by the degeneration of Purkinje cells. However, only background levels of the silver grains occured on the cerebella of agranular weaver mutant mice, suggesting that the receptor for ω-conotoxin GVIA in the cerebellum are predominantly distributed on the parellel fibers of granule cells.

AB - An in vitro autoradiographic technique has been used to localize [125I]ω-conotoxin GVIA binding sites in the brains of normal and cerebellar mutant mice. In the brains of normal mice, the highest densities of binding sites were observed at glomeruli of the olfactory bulb, cerebral cortex, caudate nucleus-putamen, hippocampus, and the nucleus of the solitary tract. Moderate densities of the silver grains occured on the granular layer of the olfactory bulb, the molecular layer of the dentate gyrus, the molecular layer of the cerebellum, and the cochlear nucleus. No specific binding appeared in the white matter or the deep nucleus of the cerebellum, the corpus callosum, the internal capsule and the external plexiform layer of the olfactory bulb. Autoradiographic studies of the cerebella of Purkinje cell degeneration (pcd) mice showed that the distribution of binding sites on the molecular layer of the cerebellum are not affected by the degeneration of Purkinje cells. However, only background levels of the silver grains occured on the cerebella of agranular weaver mutant mice, suggesting that the receptor for ω-conotoxin GVIA in the cerebellum are predominantly distributed on the parellel fibers of granule cells.

KW - Autoradiography

KW - Calcium channel

KW - Cerebellar mutant mouse

KW - ω-Conotoxin

UR - http://www.scopus.com/inward/record.url?scp=0024414065&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024414065&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(89)90004-8

DO - 10.1016/0006-8993(89)90004-8

M3 - Article

C2 - 2743150

AN - SCOPUS:0024414065

VL - 489

SP - 21

EP - 30

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -