Autoreactive T-cell responses to myeloperoxidase in patients with antineutrophil cytoplasmic antibody-associated vasculitis and in healthy individuals

Noriyuki Seta, Michiko Tajima, Shigeto Kobayashi, Yutaka Kawakami, Hiroshi Hashimoto, Masataka Kuwana

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The aim of this study was to evaluate the characteristics of autoreactive T cells to myeloperoxidase (MPO) in patients with MPO-antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Peripheral blood T cells from 15 patients with MPO-ANCA-associated vasculitis and 14 healthy individuals were cultured with three recombinant proteins that together comprised the entire MPO sequence (L, all 112 amino acids (AA) of the light chain; HI, AA 1-227 of the heavy chain; HII, AA 212-467 of the heavy chain), and the antigen-specific T-cell proliferative response was measured by 3H-thymidine incorporation. T-cell responses to MPO-L and HI were both detected in four patients and three healthy donors, and responses to MPO-HII were detected in four patients and seven healthy donors. These findings indicate that at least three independent T-cell epitopes exist on the MPO molecule. Interestingly, the patients whose T cells showed these MPO-induced responses were mainly in remission. Peripheral blood T cells reactive with MPO were primarily of the HLA-DR-restricted CD4+ phenotype. In summary, we successfully used recombinant MPO fragments to detect autoreactive CD4+ T cells to multiple MPO epitopes in blood samples from patients with MPO-ANCA-associated vasculitis and healthy individuals.

Original languageEnglish
Pages (from-to)593-600
Number of pages8
JournalModern Rheumatology
Volume18
Issue number6
DOIs
Publication statusPublished - 2008

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Antineutrophil Cytoplasmic Antibodies
Vasculitis
Peroxidase
T-Lymphocytes
Amino Acids
Blood Cells
Tissue Donors
T-Lymphocyte Epitopes
HLA-DR Antigens
Recombinant Proteins
Thymidine
Epitopes

Keywords

  • ANCA-associated vasculitis
  • MPO-reactive T cell
  • Myeloperoxidase

ASJC Scopus subject areas

  • Rheumatology

Cite this

Autoreactive T-cell responses to myeloperoxidase in patients with antineutrophil cytoplasmic antibody-associated vasculitis and in healthy individuals. / Seta, Noriyuki; Tajima, Michiko; Kobayashi, Shigeto; Kawakami, Yutaka; Hashimoto, Hiroshi; Kuwana, Masataka.

In: Modern Rheumatology, Vol. 18, No. 6, 2008, p. 593-600.

Research output: Contribution to journalArticle

Seta, Noriyuki ; Tajima, Michiko ; Kobayashi, Shigeto ; Kawakami, Yutaka ; Hashimoto, Hiroshi ; Kuwana, Masataka. / Autoreactive T-cell responses to myeloperoxidase in patients with antineutrophil cytoplasmic antibody-associated vasculitis and in healthy individuals. In: Modern Rheumatology. 2008 ; Vol. 18, No. 6. pp. 593-600.
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