Autoreactive T cells to the P3A+ isoform of AChR α subunit in myasthenia gravis

Shigeaki Suzuki; Kortaro Tanaka; Hidekata Yasuoka; Yasuo Fukuuchi; Yutaka Kawakami; Masataka Kuwana

doi:10.1016/S0165-5728(03)00078-X

Autoreactive T cells to the P3A⁺ isoform of AChR α subunit in myasthenia gravis

Shigeaki Suzuki, Kortaro Tanaka, Hidekata Yasuoka, Yasuo Fukuuchi, Yutaka Kawakami, Masataka Kuwana

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In vitro T cell proliferative response to an alternative splicing variant of acetylcholine receptor α subunit (AChRα) with the P3A exon-encoded region was examined in peripheral blood samples from 28 myasthenia gravis (MG) patients and 14 healthy donors using recombinant fragments and synthetic peptides. T cells responsive to the P3A region-specific sequences were detected in five MG patients, all of whom were late-onset disease with thymoma, but in none of healthy donors. These autoreactive T cells may be involved in the pathogenic process in a subset of MG patients.

Original language	English
Pages (from-to)	177-186
Number of pages	10
Journal	Journal of Neuroimmunology
Volume	137
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(03)00078-X
Publication status	Published - 2003 Apr

Keywords

Acetylcholine receptor α subunit
Myasthenia gravis
P3A exon
T cell
Thymoma

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/S0165-5728(03)00078-X

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title = "Autoreactive T cells to the P3A+ isoform of AChR α subunit in myasthenia gravis",

abstract = "In vitro T cell proliferative response to an alternative splicing variant of acetylcholine receptor α subunit (AChRα) with the P3A exon-encoded region was examined in peripheral blood samples from 28 myasthenia gravis (MG) patients and 14 healthy donors using recombinant fragments and synthetic peptides. T cells responsive to the P3A region-specific sequences were detected in five MG patients, all of whom were late-onset disease with thymoma, but in none of healthy donors. These autoreactive T cells may be involved in the pathogenic process in a subset of MG patients.",

keywords = "Acetylcholine receptor α subunit, Myasthenia gravis, P3A exon, T cell, Thymoma",

author = "Shigeaki Suzuki and Kortaro Tanaka and Hidekata Yasuoka and Yasuo Fukuuchi and Yutaka Kawakami and Masataka Kuwana",

note = "Funding Information: We thank Y. Okazaki and K. Kimura for their excellent technical assistance, Dr. T. Fujita and Dr. K. Dan for preparing the synthetic peptides, and Dr. A. Koto, Dr. T. Amano, Dr. N. Tanahashi, Dr. J. Hamada, and Dr. S. Nogawa for coordinating the blood specimen collection. This work was supported by a grant from the Japanese Ministry of Education, Science, Sports and Culture and the Keio University Medical Science Fund.",

year = "2003",

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doi = "10.1016/S0165-5728(03)00078-X",

language = "English",

volume = "137",

pages = "177--186",

journal = "Journal of Neuroimmunology",

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T1 - Autoreactive T cells to the P3A+ isoform of AChR α subunit in myasthenia gravis

AU - Suzuki, Shigeaki

AU - Tanaka, Kortaro

AU - Yasuoka, Hidekata

AU - Fukuuchi, Yasuo

AU - Kawakami, Yutaka

AU - Kuwana, Masataka

N1 - Funding Information: We thank Y. Okazaki and K. Kimura for their excellent technical assistance, Dr. T. Fujita and Dr. K. Dan for preparing the synthetic peptides, and Dr. A. Koto, Dr. T. Amano, Dr. N. Tanahashi, Dr. J. Hamada, and Dr. S. Nogawa for coordinating the blood specimen collection. This work was supported by a grant from the Japanese Ministry of Education, Science, Sports and Culture and the Keio University Medical Science Fund.

PY - 2003/4

Y1 - 2003/4

N2 - In vitro T cell proliferative response to an alternative splicing variant of acetylcholine receptor α subunit (AChRα) with the P3A exon-encoded region was examined in peripheral blood samples from 28 myasthenia gravis (MG) patients and 14 healthy donors using recombinant fragments and synthetic peptides. T cells responsive to the P3A region-specific sequences were detected in five MG patients, all of whom were late-onset disease with thymoma, but in none of healthy donors. These autoreactive T cells may be involved in the pathogenic process in a subset of MG patients.

AB - In vitro T cell proliferative response to an alternative splicing variant of acetylcholine receptor α subunit (AChRα) with the P3A exon-encoded region was examined in peripheral blood samples from 28 myasthenia gravis (MG) patients and 14 healthy donors using recombinant fragments and synthetic peptides. T cells responsive to the P3A region-specific sequences were detected in five MG patients, all of whom were late-onset disease with thymoma, but in none of healthy donors. These autoreactive T cells may be involved in the pathogenic process in a subset of MG patients.

KW - Acetylcholine receptor α subunit

KW - Myasthenia gravis

KW - P3A exon

KW - T cell

KW - Thymoma

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