Axenic cultivation of Entamoeba dispar in newly designed yeast extract-iron-gluconic acid-dihyrdoxyacetone-serum medium

Seiki Kobayashi, Eiko Imai, Ali Haghighi, Shaden A. Khalifa, Hiroshi Tachibana, Tsutomu Takeuchi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Yeast extract-iron-gluconic acid-dihyrdoxyacetone-serum medium that allows axenic cultivation of Entamoeba dispar was designed based on casein-free yeast extract-iron-serum (YI-S) medium, and the usefulness of the medium was assessed. The main differences from YI-S medium are replacement of glucose by gluconic acid, addition of dihydroxyacetone and D-galacturonic acid monohydrate, and sterilization by filtration. This medium promoted the axenic growth of 5 strains of E. dispar (2 strains of nonhuman primate isolates and 3 strains of human isolates). In addition, to clarify the biological basis for the growth of E. dispar in this medium, analyses of relevant enzymes on the glycolytic pathway of the amoebae as well as of the protozoans that are the best culture supplement for amoebae are being performed.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalJournal of Parasitology
Volume91
Issue number1
DOIs
Publication statusPublished - 2005 Feb 1

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

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