Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics

Hyun Jeong Yang, Yuki Sugiura, Koji Ikegami, Yoshiyuki Konishi, Mitsutoshi Setou

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Phosphatidylcholine (PC) is the most abundant component of lipid bilayers and exists in various molecular forms, through combinations of two acylated fatty acids. Arachidonic acid (AA)-containing PC (AA-PC) can be a source of AA, which is a crucial mediator of synaptic transmission and intracellular signaling. However, the distribution of AA-PC within neurons has not been indicated. In the present study, we used imaging mass spectrometry to characterize the distribution of PC species in cultured neurons of superior cervical ganglia. Intriguingly, PC species exhibited a unique distribution that was dependent on the acyl chains at the sn-2 position. In particular, we found that AA-PC is enriched within the axon and is distributed across a proximal-to-distal gradient. Inhibitors of actin dynamics (cytochalasin D and phallacidin) disrupted this gradient. This is the first report of the gradual distribution of AA-PC along the axon and its association with actin dynamics.

Original languageEnglish
Pages (from-to)5290-5300
Number of pages11
JournalJournal of Biological Chemistry
Volume287
Issue number8
DOIs
Publication statusPublished - 2012 Feb 17

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Phosphatidylcholines
Arachidonic Acid
Actins
Neurons
Axons
Cytochalasin D
Superior Cervical Ganglion
Lipid bilayers
Lipid Bilayers
Synaptic Transmission
Mass spectrometry
Mass Spectrometry
Fatty Acids
Imaging techniques

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics. / Yang, Hyun Jeong; Sugiura, Yuki; Ikegami, Koji; Konishi, Yoshiyuki; Setou, Mitsutoshi.

In: Journal of Biological Chemistry, Vol. 287, No. 8, 17.02.2012, p. 5290-5300.

Research output: Contribution to journalArticle

Yang, Hyun Jeong ; Sugiura, Yuki ; Ikegami, Koji ; Konishi, Yoshiyuki ; Setou, Mitsutoshi. / Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 8. pp. 5290-5300.
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