Abstract
Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 4518-4521 |
| Number of pages | 4 |
| Journal | Tetrahedron |
| Volume | 71 |
| Issue number | 26-27 |
| DOIs | |
| Publication status | Published - 2015 May 30 |
| Externally published | Yes |
Keywords
- Azaelectrocyclization
- Fluorescence
- Labeling
- Live cell
- Lysine
ASJC Scopus subject areas
- Biochemistry
- Drug Discovery
- Organic Chemistry
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Cite this
Ogura, A., & Tanaka, K. (2015). Azaelectrocyclization on cell surface: Convenient and general approach to chemical biology research. Tetrahedron, 71(26-27), 4518-4521. https://doi.org/10.1016/j.tet.2015.02.063