Azaelectrocyclization on cell surface: Convenient and general approach to chemical biology research

Akihiro Ogura; Katsunori Tanaka

doi:10.1016/j.tet.2015.02.063

Azaelectrocyclization on cell surface: Convenient and general approach to chemical biology research

Akihiro Ogura, Katsunori Tanaka

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions.

Original language	English
Pages (from-to)	4518-4521
Number of pages	4
Journal	Tetrahedron
Volume	71
Issue number	26-27
DOIs	https://doi.org/10.1016/j.tet.2015.02.063
Publication status	Published - 2015 May 30
Externally published	Yes

Keywords

Azaelectrocyclization
Fluorescence
Labeling
Live cell
Lysine

ASJC Scopus subject areas

Biochemistry
Drug Discovery
Organic Chemistry

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title = "Azaelectrocyclization on cell surface: Convenient and general approach to chemical biology research",

abstract = "Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions.",

keywords = "Azaelectrocyclization, Fluorescence, Labeling, Live cell, Lysine",

author = "Akihiro Ogura and Katsunori Tanaka",

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doi = "10.1016/j.tet.2015.02.063",

language = "English",

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T2 - Convenient and general approach to chemical biology research

AU - Ogura, Akihiro

AU - Tanaka, Katsunori

PY - 2015/5/30

Y1 - 2015/5/30

N2 - Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions.

AB - Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions.

KW - Azaelectrocyclization

KW - Fluorescence

KW - Labeling

KW - Live cell

KW - Lysine

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M3 - Article

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EP - 4521

JO - Tetrahedron

JF - Tetrahedron

SN - 0040-4020

IS - 26-27

ER -