Abstract
Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 4518-4521 |
| Number of pages | 4 |
| Journal | Tetrahedron |
| Volume | 71 |
| Issue number | 26-27 |
| DOIs | |
| Publication status | Published - 2015 Jan 13 |
| Externally published | Yes |
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Keywords
- Azaelectrocyclization
- Fluorescence
- Labeling
- Live cell
- Lysine
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry
- Drug Discovery
Cite this
Azaelectrocyclization on cell surface : Convenient and general approach to chemical biology research. / Ogura, Akihiro; Tanaka, Katsunori.
In: Tetrahedron, Vol. 71, No. 26-27, 13.01.2015, p. 4518-4521.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Azaelectrocyclization on cell surface
T2 - Convenient and general approach to chemical biology research
AU - Ogura, Akihiro
AU - Tanaka, Katsunori
PY - 2015/1/13
Y1 - 2015/1/13
N2 - Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions.
AB - Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions.
KW - Azaelectrocyclization
KW - Fluorescence
KW - Labeling
KW - Live cell
KW - Lysine
UR - http://www.scopus.com/inward/record.url?scp=84930082000&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930082000&partnerID=8YFLogxK
U2 - 10.1016/j.tet.2015.02.063
DO - 10.1016/j.tet.2015.02.063
M3 - Article
AN - SCOPUS:84930082000
VL - 71
SP - 4518
EP - 4521
JO - Tetrahedron
JF - Tetrahedron
SN - 0040-4020
IS - 26-27
ER -