Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA

Ryo Iwaoka, Takashi Nagata, Kengo Tsuda, Takao Imai, Hideyuki Okano, Naohiro Kobayashi, Masato Katahira

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Musashi1 (Msi1) is an RNA-binding protein that is involved in cell fate determination. Here, we report the 1H, 15N, and 13C resonance assignments of Msi1 second RNA-binding domain in free form and in complex with RNA. The assignments can be utilized for NMR structure and dynamics analyses of the Msi1:RNA complex, and moreover, for chemical shift perturbation analyses to evaluate the binding of potential small molecule inhibitors against Msi1:RNA interaction.

Original languageEnglish
Pages (from-to)265-268
Number of pages4
JournalBiomolecular NMR Assignments
Volume11
Issue number2
DOIs
Publication statusPublished - 2017 Oct 1

Keywords

  • Cancer development
  • Cell fate determination
  • Musashi
  • RBD
  • RNA-binding protein
  • RRM

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

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