Background norepinephrine primes astrocytic calcium responses to subsequent norepinephrine stimuli in the cerebral cortex

Mutsuo Nuriya, Miyabi Takeuchi, Masato Yasui

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Norepinephrine (NE) levels in the cerebral cortex are regulated in two modes; the brain state is correlated with slow changes in background NE concentration, while salient stimuli induce transient NE spikes. Previous studies have revealed their diverse neuromodulatory actions; however, the modulatory role of NE on astrocytic activity has been poorly characterized thus far. In this study, we evaluated the modulatory action of background NE on astrocytic responses to subsequent stimuli, using two-photon calcium imaging of acute murine cortical brain slices. We find that subthreshold background NE significantly augments calcium responses to subsequent pulsed NE stimulation in astrocytes. This priming effect is independent of neuronal activity and is mediated by the activation of β-adrenoceptors and the downstream cAMP pathway. These results indicate that background NE primes astrocytes for subsequent calcium responses to NE stimulation and suggest a novel gliomodulatory role for brain state-dependent background NE in the cerebral cortex.

Original languageEnglish
Pages (from-to)732-738
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume483
Issue number1
DOIs
Publication statusPublished - 2017 Jan 29

Fingerprint

Cerebral Cortex
Norepinephrine
Calcium
Brain
Astrocytes
Photons
Adrenergic Receptors
Chemical activation
Imaging techniques

Keywords

  • Astrocyte
  • Calcium
  • Cerebral cortex
  • Norepinephrine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{9b4b086543a84f87b55747c7cd7a0ed1,
title = "Background norepinephrine primes astrocytic calcium responses to subsequent norepinephrine stimuli in the cerebral cortex",
abstract = "Norepinephrine (NE) levels in the cerebral cortex are regulated in two modes; the brain state is correlated with slow changes in background NE concentration, while salient stimuli induce transient NE spikes. Previous studies have revealed their diverse neuromodulatory actions; however, the modulatory role of NE on astrocytic activity has been poorly characterized thus far. In this study, we evaluated the modulatory action of background NE on astrocytic responses to subsequent stimuli, using two-photon calcium imaging of acute murine cortical brain slices. We find that subthreshold background NE significantly augments calcium responses to subsequent pulsed NE stimulation in astrocytes. This priming effect is independent of neuronal activity and is mediated by the activation of β-adrenoceptors and the downstream cAMP pathway. These results indicate that background NE primes astrocytes for subsequent calcium responses to NE stimulation and suggest a novel gliomodulatory role for brain state-dependent background NE in the cerebral cortex.",
keywords = "Astrocyte, Calcium, Cerebral cortex, Norepinephrine",
author = "Mutsuo Nuriya and Miyabi Takeuchi and Masato Yasui",
year = "2017",
month = "1",
day = "29",
doi = "10.1016/j.bbrc.2016.12.073",
language = "English",
volume = "483",
pages = "732--738",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Background norepinephrine primes astrocytic calcium responses to subsequent norepinephrine stimuli in the cerebral cortex

AU - Nuriya, Mutsuo

AU - Takeuchi, Miyabi

AU - Yasui, Masato

PY - 2017/1/29

Y1 - 2017/1/29

N2 - Norepinephrine (NE) levels in the cerebral cortex are regulated in two modes; the brain state is correlated with slow changes in background NE concentration, while salient stimuli induce transient NE spikes. Previous studies have revealed their diverse neuromodulatory actions; however, the modulatory role of NE on astrocytic activity has been poorly characterized thus far. In this study, we evaluated the modulatory action of background NE on astrocytic responses to subsequent stimuli, using two-photon calcium imaging of acute murine cortical brain slices. We find that subthreshold background NE significantly augments calcium responses to subsequent pulsed NE stimulation in astrocytes. This priming effect is independent of neuronal activity and is mediated by the activation of β-adrenoceptors and the downstream cAMP pathway. These results indicate that background NE primes astrocytes for subsequent calcium responses to NE stimulation and suggest a novel gliomodulatory role for brain state-dependent background NE in the cerebral cortex.

AB - Norepinephrine (NE) levels in the cerebral cortex are regulated in two modes; the brain state is correlated with slow changes in background NE concentration, while salient stimuli induce transient NE spikes. Previous studies have revealed their diverse neuromodulatory actions; however, the modulatory role of NE on astrocytic activity has been poorly characterized thus far. In this study, we evaluated the modulatory action of background NE on astrocytic responses to subsequent stimuli, using two-photon calcium imaging of acute murine cortical brain slices. We find that subthreshold background NE significantly augments calcium responses to subsequent pulsed NE stimulation in astrocytes. This priming effect is independent of neuronal activity and is mediated by the activation of β-adrenoceptors and the downstream cAMP pathway. These results indicate that background NE primes astrocytes for subsequent calcium responses to NE stimulation and suggest a novel gliomodulatory role for brain state-dependent background NE in the cerebral cortex.

KW - Astrocyte

KW - Calcium

KW - Cerebral cortex

KW - Norepinephrine

UR - http://www.scopus.com/inward/record.url?scp=85007579913&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007579913&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2016.12.073

DO - 10.1016/j.bbrc.2016.12.073

M3 - Article

C2 - 27965089

AN - SCOPUS:85007579913

VL - 483

SP - 732

EP - 738

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -