Bacteria manipulation using dielectrophoresis for efficient screening

T. Kano, T. Inaba, Norihisa Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper we demonstrate a microfluidic device that immobilizes microbes into microporous carrier using positive dielectrophoresis (DEP). This device can characterize bacteria, while precisely controlling the reaction conditions and the number of bacteria involved in the biochemical reaction, and can effectively screen them to efficiently produce useful chemicals by fermentation, such as organic acids, antibiotic drugs, and foods. We chose microbes belonging to Corynebacterium group as samples. First, as preparing for using the microfluidic device, we experimentally deduced effective DEP frequency of 10 MHz, when live C. glutamicum was preferably driven to the areas with stronger electric fields. Second, we characterized the microfluidic device and found that the device could immobilize microbes into micro pores and evaluate the production capacity of a single bacrerium. As the demonstration, we used the developed device to characterize microbes under various environments and evaluate biological activity.

Original languageEnglish
Title of host publicationIEEE International Conference on Intelligent Robots and Systems
Pages3247-3252
Number of pages6
DOIs
Publication statusPublished - 2012
Event25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012 - Vilamoura, Algarve, Portugal
Duration: 2012 Oct 72012 Oct 12

Other

Other25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012
CountryPortugal
CityVilamoura, Algarve
Period12/10/712/10/12

Fingerprint

Electrophoresis
Microfluidics
Bacteria
Screening
Organic acids
Antibiotics
Bioactivity
Fermentation
Demonstrations
Electric fields

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Software
  • Computer Vision and Pattern Recognition
  • Computer Science Applications

Cite this

Kano, T., Inaba, T., & Miki, N. (2012). Bacteria manipulation using dielectrophoresis for efficient screening. In IEEE International Conference on Intelligent Robots and Systems (pp. 3247-3252). [6386197] https://doi.org/10.1109/IROS.2012.6386197

Bacteria manipulation using dielectrophoresis for efficient screening. / Kano, T.; Inaba, T.; Miki, Norihisa.

IEEE International Conference on Intelligent Robots and Systems. 2012. p. 3247-3252 6386197.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kano, T, Inaba, T & Miki, N 2012, Bacteria manipulation using dielectrophoresis for efficient screening. in IEEE International Conference on Intelligent Robots and Systems., 6386197, pp. 3247-3252, 25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012, Vilamoura, Algarve, Portugal, 12/10/7. https://doi.org/10.1109/IROS.2012.6386197
Kano T, Inaba T, Miki N. Bacteria manipulation using dielectrophoresis for efficient screening. In IEEE International Conference on Intelligent Robots and Systems. 2012. p. 3247-3252. 6386197 https://doi.org/10.1109/IROS.2012.6386197
Kano, T. ; Inaba, T. ; Miki, Norihisa. / Bacteria manipulation using dielectrophoresis for efficient screening. IEEE International Conference on Intelligent Robots and Systems. 2012. pp. 3247-3252
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