Abstract
In this paper we demonstrate a microfluidic device that immobilizes microbes into microporous carrier using positive dielectrophoresis (DEP). This device can characterize bacteria, while precisely controlling the reaction conditions and the number of bacteria involved in the biochemical reaction, and can effectively screen them to efficiently produce useful chemicals by fermentation, such as organic acids, antibiotic drugs, and foods. We chose microbes belonging to Corynebacterium group as samples. First, as preparing for using the microfluidic device, we experimentally deduced effective DEP frequency of 10 MHz, when live C. glutamicum was preferably driven to the areas with stronger electric fields. Second, we characterized the microfluidic device and found that the device could immobilize microbes into micro pores and evaluate the production capacity of a single bacrerium. As the demonstration, we used the developed device to characterize microbes under various environments and evaluate biological activity.
| Original language | English |
|---|---|
| Title of host publication | IEEE International Conference on Intelligent Robots and Systems |
| Pages | 3247-3252 |
| Number of pages | 6 |
| DOIs | |
| Publication status | Published - 2012 |
| Event | 25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012 - Vilamoura, Algarve, Portugal Duration: 2012 Oct 7 → 2012 Oct 12 |
Other
| Other | 25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012 |
|---|---|
| Country | Portugal |
| City | Vilamoura, Algarve |
| Period | 12/10/7 → 12/10/12 |
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ASJC Scopus subject areas
- Control and Systems Engineering
- Software
- Computer Vision and Pattern Recognition
- Computer Science Applications
Cite this
Bacteria manipulation using dielectrophoresis for efficient screening. / Kano, T.; Inaba, T.; Miki, Norihisa.
IEEE International Conference on Intelligent Robots and Systems. 2012. p. 3247-3252 6386197.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Bacteria manipulation using dielectrophoresis for efficient screening
AU - Kano, T.
AU - Inaba, T.
AU - Miki, Norihisa
PY - 2012
Y1 - 2012
N2 - In this paper we demonstrate a microfluidic device that immobilizes microbes into microporous carrier using positive dielectrophoresis (DEP). This device can characterize bacteria, while precisely controlling the reaction conditions and the number of bacteria involved in the biochemical reaction, and can effectively screen them to efficiently produce useful chemicals by fermentation, such as organic acids, antibiotic drugs, and foods. We chose microbes belonging to Corynebacterium group as samples. First, as preparing for using the microfluidic device, we experimentally deduced effective DEP frequency of 10 MHz, when live C. glutamicum was preferably driven to the areas with stronger electric fields. Second, we characterized the microfluidic device and found that the device could immobilize microbes into micro pores and evaluate the production capacity of a single bacrerium. As the demonstration, we used the developed device to characterize microbes under various environments and evaluate biological activity.
AB - In this paper we demonstrate a microfluidic device that immobilizes microbes into microporous carrier using positive dielectrophoresis (DEP). This device can characterize bacteria, while precisely controlling the reaction conditions and the number of bacteria involved in the biochemical reaction, and can effectively screen them to efficiently produce useful chemicals by fermentation, such as organic acids, antibiotic drugs, and foods. We chose microbes belonging to Corynebacterium group as samples. First, as preparing for using the microfluidic device, we experimentally deduced effective DEP frequency of 10 MHz, when live C. glutamicum was preferably driven to the areas with stronger electric fields. Second, we characterized the microfluidic device and found that the device could immobilize microbes into micro pores and evaluate the production capacity of a single bacrerium. As the demonstration, we used the developed device to characterize microbes under various environments and evaluate biological activity.
UR - http://www.scopus.com/inward/record.url?scp=84872334035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872334035&partnerID=8YFLogxK
U2 - 10.1109/IROS.2012.6386197
DO - 10.1109/IROS.2012.6386197
M3 - Conference contribution
SN - 9781467317375
SP - 3247
EP - 3252
BT - IEEE International Conference on Intelligent Robots and Systems
ER -