BAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse embryonic stem cells

Zhijiang Yan, Zhong Wang, Lioudmila Sharova, Alexei A. Sharov, Chen Ling, Yulan Piao, Kazuhiro Aiba, Ryo Matoba, Weidong Wang, Minoru Ko

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Whether SWI/SNF chromatin remodeling complexes play roles in embryonic stem (ES) cells remains unknown. Here we show that SWI/SNF complexes are present in mouse ES cells, and their composition is dynamically regulated upon induction of ES cell differentiation. For example, the SWI/SNF purified from undifferentiated ES cells contains a high level of BAF155 and a low level of BAF170 (both of which are homologs of yeast SWI3 protein), whereas that from differentiated cells contains nearly equal amounts of both. Moreover, the levels of BAF250A and BAF250B decrease during the differentiation of ES cells, whereas that of BRM increases. The altered expression of SWI/SNF components hinted that these complexes could play roles in ES cell maintenance or differentiation. We therefore generated ES cells with biallelic inactivation of BAF250B and found that these cells display a reduced proliferation rate and an abnormal cell cycle. Importantly, these cells are deficient in the self-renewal capacity of undifferentiated ES cells and exhibit certain phenotypes of differentiated cells, including reduced expression of several pluripotency-related genes and increased expression of some differentiation-related genes. These data suggest that the BAF250B-associated SWI/SNF is essential for mouse ES cells to maintain their normal proliferation and pluripotency. The work presented here underscores the importance of SWI/SNF chromatin remodeling complexes in pluripotent stem cells.

Original languageEnglish
Pages (from-to)1155-1165
Number of pages11
JournalStem Cells
Volume26
Issue number5
DOIs
Publication statusPublished - 2008 May
Externally publishedYes

Fingerprint

Chromatin Assembly and Disassembly
Embryonic Stem Cells
Pluripotent Stem Cells
Fungal Proteins
Mouse Embryonic Stem Cells
Cell Differentiation
Cell Cycle
Maintenance
Phenotype
Gene Expression
Genes

Keywords

  • ARID1B
  • BAF250B
  • Chromatin remodeling
  • DNA microarray
  • Embryonic stem cells
  • Gene targeting
  • SWI/SNF

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine
  • Medicine(all)

Cite this

BAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse embryonic stem cells. / Yan, Zhijiang; Wang, Zhong; Sharova, Lioudmila; Sharov, Alexei A.; Ling, Chen; Piao, Yulan; Aiba, Kazuhiro; Matoba, Ryo; Wang, Weidong; Ko, Minoru.

In: Stem Cells, Vol. 26, No. 5, 05.2008, p. 1155-1165.

Research output: Contribution to journalArticle

Yan, Z, Wang, Z, Sharova, L, Sharov, AA, Ling, C, Piao, Y, Aiba, K, Matoba, R, Wang, W & Ko, M 2008, 'BAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse embryonic stem cells', Stem Cells, vol. 26, no. 5, pp. 1155-1165. https://doi.org/10.1634/stemcells.2007-0846
Yan, Zhijiang ; Wang, Zhong ; Sharova, Lioudmila ; Sharov, Alexei A. ; Ling, Chen ; Piao, Yulan ; Aiba, Kazuhiro ; Matoba, Ryo ; Wang, Weidong ; Ko, Minoru. / BAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse embryonic stem cells. In: Stem Cells. 2008 ; Vol. 26, No. 5. pp. 1155-1165.
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