Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis

Hideyuki Nishizawa; Mami Iida; Yoshihiro Abe

doi:10.2116/analsci.1.451

Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis

Hideyuki Nishizawa, Mami Iida, Yoshihiro Abe

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

When we used an acidic solution of BPB as a marker for tracking, we observed that the band of the dye sharpened to become a very thin line. We supposed that the sample proteins in the gel should be similarly concentrated by the action of acid, and that the resolution should be improved. Among the acids we assessed, most of which sharpened the BPB band, 2 M acetic acid showed the proper band sharpening action and brought about the improvement in resolution.

Original language	English
Pages (from-to)	451-454
Number of pages	4
Journal	analytical sciences
Volume	1
Issue number	5
DOIs	https://doi.org/10.2116/analsci.1.451
Publication status	Published - 1985
Externally published	Yes

Keywords

Bromophenol Blue (BPB)
Polyacrylamide gel electrophoresis (PAGE)
band sharpening

ASJC Scopus subject areas

Analytical Chemistry

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10.2116/analsci.1.451

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abstract = "When we used an acidic solution of BPB as a marker for tracking, we observed that the band of the dye sharpened to become a very thin line. We supposed that the sample proteins in the gel should be similarly concentrated by the action of acid, and that the resolution should be improved. Among the acids we assessed, most of which sharpened the BPB band, 2 M acetic acid showed the proper band sharpening action and brought about the improvement in resolution.",

keywords = "Bromophenol Blue (BPB), Polyacrylamide gel electrophoresis (PAGE), band sharpening",

author = "Hideyuki Nishizawa and Mami Iida and Yoshihiro Abe",

year = "1985",

doi = "10.2116/analsci.1.451",

language = "English",

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journal = "Analytical Sciences",

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T1 - Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis

AU - Nishizawa, Hideyuki

AU - Iida, Mami

AU - Abe, Yoshihiro

PY - 1985

Y1 - 1985

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AB - When we used an acidic solution of BPB as a marker for tracking, we observed that the band of the dye sharpened to become a very thin line. We supposed that the sample proteins in the gel should be similarly concentrated by the action of acid, and that the resolution should be improved. Among the acids we assessed, most of which sharpened the BPB band, 2 M acetic acid showed the proper band sharpening action and brought about the improvement in resolution.

KW - Bromophenol Blue (BPB)

KW - Polyacrylamide gel electrophoresis (PAGE)

KW - band sharpening

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DO - 10.2116/analsci.1.451

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ER -

Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis

Abstract

Keywords

ASJC Scopus subject areas

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