Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis

Hideyuki Nishizawa, Mami Iida, Yoshihiro Abe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

When we used an acidic solution of BPB as a marker for tracking, we observed that the band of the dye sharpened to become a very thin line. We supposed that the sample proteins in the gel should be similarly concentrated by the action of acid, and that the resolution should be improved. Among the acids we assessed, most of which sharpened the BPB band, 2 M acetic acid showed the proper band sharpening action and brought about the improvement in resolution.

Original languageEnglish
Pages (from-to)451-454
Number of pages4
JournalAnalytical Sciences
Volume1
Issue number5
DOIs
Publication statusPublished - 1985

Fingerprint

Electrophoresis
Acids
Acetic Acid
Coloring Agents
Gels
Proteins
polyacrylamide gels

Keywords

  • band sharpening
  • Bromophenol Blue (BPB)
  • Polyacrylamide gel electrophoresis (PAGE)

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis. / Nishizawa, Hideyuki; Iida, Mami; Abe, Yoshihiro.

In: Analytical Sciences, Vol. 1, No. 5, 1985, p. 451-454.

Research output: Contribution to journalArticle

Nishizawa, Hideyuki ; Iida, Mami ; Abe, Yoshihiro. / Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis. In: Analytical Sciences. 1985 ; Vol. 1, No. 5. pp. 451-454.
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