Base editors for simultaneous introduction of C-to-T and A-to-G mutations

Rina C. Sakata, Soh Ishiguro, Hideto Mori, Mamoru Tanaka, Kenji Tatsuno, Hiroki Ueda, Shogo Yamamoto, Motoaki Seki, Nanami Masuyama, Keiji Nishida, Hiroshi Nishimasu, Kazuharu Arakawa, Akihiko Kondo, Osamu Nureki, Masaru Tomita, Hiroyuki Aburatani, Nozomu Yachie

Research output: Contribution to journalArticle

Abstract

We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. On-target as well as DNA and RNA off-target activities of Target-ACEmax were similar to those of existing single-function base editors.

Original languageEnglish
JournalNature Biotechnology
DOIs
Publication statusAccepted/In press - 2020 Jan 1

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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  • Cite this

    Sakata, R. C., Ishiguro, S., Mori, H., Tanaka, M., Tatsuno, K., Ueda, H., Yamamoto, S., Seki, M., Masuyama, N., Nishida, K., Nishimasu, H., Arakawa, K., Kondo, A., Nureki, O., Tomita, M., Aburatani, H., & Yachie, N. (Accepted/In press). Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0509-0