Basement membrane-like matrix sponge for the three-dimensional proliferation culture of differentiated retinal horizontal interneurons

Itsuki Ajioka, Shizuko Ichinose, Kazunori Nakajima, Hidehiro Mizusawa

Research output: Contribution to journalArticle

8 Citations (Scopus)


As the neurons in the central nervous system (CNS) form a neuronal network in a three-dimensional (3D) manner, a 3D proliferation culture system for differentiated neurons has been desired. Although differentiated neurons were previously thought to never proliferate, differentiated horizontal interneurons of Rb-/-; p107+. /-; p130-/- (p107-single) retina clonally proliferated without dedifferentiation in vivo [1]. In the present study, we developed a basement membrane-like matrix sponge (BM-sponge) for the 3D proliferation culture of differentiated horizontal interneurons. p107-single horizontal interneurons, but not other types of retinal neurons, proliferated in the BM-sponge in a 3D manner. These interneurons expressed presynaptic marker and developed synaptic vesicles. These data demonstrated that p107-single horizontal interneurons cultured in the BM-sponge proliferate while maintaining their differentiated features. We described here the 3D proliferation culture system for differentiated neurons.

Original languageEnglish
Pages (from-to)5765-5772
Number of pages8
Issue number25
Publication statusPublished - 2011 Sep 1



  • Cell culture
  • Cell proliferation
  • ECM (extra-cellular matrix)
  • Neural cell

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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