Basic fibroblast growth factor as a potential stent coating material inducing endothelial cell proliferation

Noriko Kitamura, Terumitsu Hasebe, Tomohiro Matsumoto, Atsushi Hotta, Tetsuya Suzuki, Takuji Yamagami, Hitoshi Terada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aim: The use of drug-eluting stents has reduced the incidence of in-stent restenosis following percutaneous coronary intervention; however, almost all drugs eluting from polymers on stents induce antiproliferative effects on vascular endothelial and vascular smooth muscle cells. Due to injury of the endothelium and delayed reendothelialization, the risk of thrombosis increases over time. Enhancing rapid reendothelialization after stent placement is important for solving these problems. Basic fibroblast growth factor (bFGF) is one of the most important growth factors involved in vascular lesion formation. In this study, we evaluated the potential of bFGF as a stent coating promoting endothelial cell proliferation. Methods: Human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HASMCs) were cultured with various doses of bFGF in vitro, and the effects of bFGF on the degree of cell proliferation and migration were monitored. We also investigated the effects of bFGF on the protein expression of endothelial nitric oxide synthase (eNOS) in HUVECs using Western blotting. Results: Cell proliferation and migration were promoted in HUVECs by bFGF in a dose- and timedependent manner. On the other hand, bFGF stimulation had little effect on the HASMCs. Basic FGF increased the eNOS protein levels in the HUVECs, with a maximum at 10 ng/mL followed by a decline at 100 ng/mL. Conclusions: Basic FGF is a possible candidate stent coating and/or eluting drug material stimulating endothelial cell proliferation and early reendothelialization without excessive vascular smooth muscle cell proliferation.

Original languageEnglish
Pages (from-to)477-485
Number of pages9
JournalJournal of Atherosclerosis and Thrombosis
Volume21
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Stents
Endothelial cells
Cell proliferation
Fibroblast Growth Factor 2
Endothelial Cells
Cell Proliferation
Human Umbilical Vein Endothelial Cells
Coatings
Smooth Muscle Myocytes
Muscle
Nitric Oxide Synthase Type III
Vascular Smooth Muscle
Cell Movement
Blood Vessels
Cells
Pharmaceutical Preparations
Drug-Eluting Stents
Percutaneous Coronary Intervention
Endothelium
Intercellular Signaling Peptides and Proteins

Keywords

  • Basic fibroblast growth factor
  • Human umbilical vein endothelial cells
  • Reendothelialization
  • Stent

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Internal Medicine
  • Biochemistry, medical

Cite this

Basic fibroblast growth factor as a potential stent coating material inducing endothelial cell proliferation. / Kitamura, Noriko; Hasebe, Terumitsu; Matsumoto, Tomohiro; Hotta, Atsushi; Suzuki, Tetsuya; Yamagami, Takuji; Terada, Hitoshi.

In: Journal of Atherosclerosis and Thrombosis, Vol. 21, No. 5, 2014, p. 477-485.

Research output: Contribution to journalArticle

Kitamura, Noriko ; Hasebe, Terumitsu ; Matsumoto, Tomohiro ; Hotta, Atsushi ; Suzuki, Tetsuya ; Yamagami, Takuji ; Terada, Hitoshi. / Basic fibroblast growth factor as a potential stent coating material inducing endothelial cell proliferation. In: Journal of Atherosclerosis and Thrombosis. 2014 ; Vol. 21, No. 5. pp. 477-485.
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