Basic studies on the clinical applications of platelet-rich plasma

Masaki Yazawa, Hisao Ogata, Tatsuo Nakajima, Taisuke Mori, Naohide Watanabe, Makoto Handa

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Platelets, which contain many growth factors such as platelet-derived growth factor (PDGF) and transforming growth factor-β (TGF-β), are being used in clinical applications as platelet-rich plasma (PRP). Only a few studies, however, have been conducted on the growth factors present in PRP and on the clinical applications using the drug delivery system (DDS). For the purpose of clinical application, we first modified the PRP preparation method and assessed the amounts of growth factors contained in the human platelet concentrates. Furthermore, we assessed fibrin glue as a DDS of platelet concentrates. Platelet precipitations were made by twice centrifuging human whole blood. The precipitated platelet was resuspended to yield the platelet concentrates. The growth factor concentrations were measured. Fibrin glue sheets containing this platelet concentrate were implanted in rabbit pinna and samples were obtained for immunostaining (anti-PDGF antibody) to assess the use of PRP over time using the fibrin glue as the DDS. The mean concentration of growth factors present in the platelet concentrates was three times or greater than that of conventional PRP. Furthermore, the results indicated that when the platelet concentrate was used with fibrin glue as a carrier, the contents were released over a period of about 1 week. This raises the possibility that this system may be useful in clinical applications.

Original languageEnglish
Pages (from-to)509-518
Number of pages10
JournalCell Transplantation
Volume12
Issue number5
DOIs
Publication statusPublished - 2003 Jan 1

Keywords

  • Drug delivery system
  • Fibrin glue
  • Platelet-rich plasma
  • Platlet-derived growth factor

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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