Basic study on pulse-intensity-domain depth-controlled Photodynamic Therapy for transurethral prostate cancer

Sayaka Ohmori, Kensuke Masuda, Yuko Yamakawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Photodynamic therapy (PDT) is promising modality for cancer. Prostate cancer is the most common cancer in USA. We proposed transurethral prostate cancer treatment using the pulse-intensity-domain depth-controlled PDT to preserve urethra wall. We have found that photocytotoxicity has been suppressed under high-intensity pulsed excitation with the second generation photosensitizers. We aim to apply this effect to form intact portion on the surface of the irradiated field. Irradiation condition dependence of photocytotoxicity of rat prostate cancer cell line R3327-AT-1 was investigated with two clinical photosensitizers, Porfimer sodium and Talaporfin sodium. A pulsed laser was irradiated with the power energy density ranging from 1.25 to 10 mJ/cm2. Near-infrared luminescence from singlet oxygen in the solution of those two photosensitizers was measured transiently. We performed PDT against a rat subcutaneous prostate tumor mode with Talaporfin sodium (2mg/kg) injected intravenously 1 h prior to the irradiation. The laser was irradiated with the power energy density 2.5 or 10 mW/cm2, with the total fluence of 50 J/cm2. Photocytotoxicity in vitro and the singlet oxygen generation were both suppressed with the 10mJ/cm2 irradiation with Talaporfin sodium, while these with Porfimer sodium were kept relatively constant. The surface of the irradiated field of 1mm in thickness remained intact, while the tumor damaged layer of 1.3 mm in thickness was obtained in the case of 10mJ/cm2 irradiation. We think Talaporfin sodium has high sensitivity to the pulse energy density, which might be useful to realize urethra preserved PDT for prostate cancer.

Original languageEnglish
Title of host publicationOptical Methods for Tumor Treatment and Detection
Subtitle of host publicationMechanisms and Techniques in Photodynamic Therapy XV
DOIs
Publication statusPublished - 2006 Jun 30
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV - San Jose, CA, United States
Duration: 2006 Jan 212006 Jan 22

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6139
ISSN (Print)1605-7422

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV
CountryUnited States
CitySan Jose, CA
Period06/1/2106/1/22

Keywords

  • High intensity
  • Prostate cancer
  • Pulsed excitation
  • Surface prevention

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Ohmori, S., Masuda, K., Yamakawa, Y., & Arai, T. (2006). Basic study on pulse-intensity-domain depth-controlled Photodynamic Therapy for transurethral prostate cancer. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV [61390T] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6139). https://doi.org/10.1117/12.647768