Basonuclin in murine corneal and lens epithelia correlates with cellular maturation and proliferative ability

Hung Tseng, Kyoichi Matsuzaki, Robert M. Lavker

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Basonuclin is a zinc finger protein with highly restricted tissue distribution. It has been found in abundance only in keratinocytes of stratified epithelia and the germ cells of the testis and ovary. We studied the expression pattern of basonuclin in relation to cellular proliferation and differentiation in murine corneal and lens epithelia, two self-renewing tissues in the eye which contain cells that proliferate throughout life. Mouse corneal and lens epithelial cells at various stages of development were labeled with BrdU for 90 min to detect cells in S phase and to establish proliferative rates. Whole eyes of mouse or rat were processed for frozen sections and cellular basonuclin was detected by either a rabbit anti-mouse- or a rabbit anti-human-basonuclin antibody. Basonuclin was expressed in virtually all cells in the basal layer of corneal epithelium and in the pre-equatorial lens epithelium, the respective proliferative compartments of adult corneal and lens epithelia. Basonuclin expression in corneal epithelium began at post-natal life day 4, first in a few cells and then spread to virtually all basal cells at day 20. Basonuclin was consistently absent in limbal epithelium. Lens basonuclin, which was detected earlier than that of the cornea, was confined to the pre-equatorial epithelium and was absent in equatorial cells that expressed p57(KIP2), an early differentiation marker for these cells. An important distinction between corneal and lens basonuclin is that the former is predominantly nuclear whereas the latter cytoplasmic.

Original languageEnglish
Pages (from-to)221-227
Number of pages7
JournalDifferentiation
Volume65
Issue number4
Publication statusPublished - 1999
Externally publishedYes

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Corneal Epithelium
Lenses
Epithelium
Rabbits
Zinc Fingers
Differentiation Antigens
Frozen Sections
Tissue Distribution
Bromodeoxyuridine
S Phase
Keratinocytes
Germ Cells
Cornea
Testis
Ovary
Epithelial Cells
Cell Proliferation
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Basonuclin in murine corneal and lens epithelia correlates with cellular maturation and proliferative ability. / Tseng, Hung; Matsuzaki, Kyoichi; Lavker, Robert M.

In: Differentiation, Vol. 65, No. 4, 1999, p. 221-227.

Research output: Contribution to journalArticle

Tseng, Hung ; Matsuzaki, Kyoichi ; Lavker, Robert M. / Basonuclin in murine corneal and lens epithelia correlates with cellular maturation and proliferative ability. In: Differentiation. 1999 ; Vol. 65, No. 4. pp. 221-227.
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