Basophils regulate the recruitment of eosinophils in a murine model of irritant contact dermatitis

Chisa Nakashima, Atsushi Otsuka, Akihiko Kitoh, Tetsuya Honda, Gyohei Egawa, Saeko Nakajima, Satoshi Nakamizo, Makoto Arita, Masato Kubo, Yoshiki Miyachi, Kenji Kabashima

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Background Although eosinophils have been detected in several human skin diseases in the vicinity of basophils, how eosinophils infiltrate the skin and the role of eosinophils in the development of skin inflammation have yet to be examined. Objective Using murine irritant contact dermatitis (ICD) as a model, we sought to clarify the roles of eosinophils in ICD and the underlying mechanism of eosinophil infiltration of the skin. Methods We induced croton oil-induced ICD in eosinophil-deficient ΔdblGATA mice with or without a reactive oxygen species (ROS) inhibitor. We performed cocultivation with fibroblasts and bone marrow-derived basophils and evaluated eosinophil migration using a chemotaxis assay. Results ICD responses were significantly attenuated in the absence of eosinophils or by treatment with the ROS inhibitor. ROS was produced abundantly by eosinophils, and both basophils and eosinophils were detected in human and murine ICD skin lesions. In coculture experiments, basophils attracted eosinophils, especially in the presence of fibroblasts. Moreover, basophils produced IL-4 and TNF-α in contact with fibroblasts and promoted the expression of eotaxin/CCL11 from fibroblasts in vitro. Conclusion Eosinophils mediated the development of murine ICD, possibly through ROS production. Recruitment of eosinophils into the skin was induced by basophils in cooperation with fibroblasts. Our findings introduce the novel concept that basophils promote the recruitment of eosinophils into the skin through fibroblasts in the development of skin inflammation.

Original languageEnglish
JournalJournal of Allergy and Clinical Immunology
Volume134
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Irritant Dermatitis
Basophils
Contact Dermatitis
Eosinophils
Fibroblasts
Skin
Reactive Oxygen Species
Coculture Techniques
Croton Oil
Inflammation
Chemotaxis

Keywords

  • basophil
  • Eosinophil
  • eotaxin/CCL11
  • fibroblast
  • irritant contact dermatitis
  • RANTES/CCL5
  • reactive oxygen species
  • tumor necrosis factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Medicine(all)

Cite this

Basophils regulate the recruitment of eosinophils in a murine model of irritant contact dermatitis. / Nakashima, Chisa; Otsuka, Atsushi; Kitoh, Akihiko; Honda, Tetsuya; Egawa, Gyohei; Nakajima, Saeko; Nakamizo, Satoshi; Arita, Makoto; Kubo, Masato; Miyachi, Yoshiki; Kabashima, Kenji.

In: Journal of Allergy and Clinical Immunology, Vol. 134, No. 1, 2014.

Research output: Contribution to journalArticle

Nakashima, C, Otsuka, A, Kitoh, A, Honda, T, Egawa, G, Nakajima, S, Nakamizo, S, Arita, M, Kubo, M, Miyachi, Y & Kabashima, K 2014, 'Basophils regulate the recruitment of eosinophils in a murine model of irritant contact dermatitis', Journal of Allergy and Clinical Immunology, vol. 134, no. 1. https://doi.org/10.1016/j.jaci.2014.02.026
Nakashima, Chisa ; Otsuka, Atsushi ; Kitoh, Akihiko ; Honda, Tetsuya ; Egawa, Gyohei ; Nakajima, Saeko ; Nakamizo, Satoshi ; Arita, Makoto ; Kubo, Masato ; Miyachi, Yoshiki ; Kabashima, Kenji. / Basophils regulate the recruitment of eosinophils in a murine model of irritant contact dermatitis. In: Journal of Allergy and Clinical Immunology. 2014 ; Vol. 134, No. 1.
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abstract = "Background Although eosinophils have been detected in several human skin diseases in the vicinity of basophils, how eosinophils infiltrate the skin and the role of eosinophils in the development of skin inflammation have yet to be examined. Objective Using murine irritant contact dermatitis (ICD) as a model, we sought to clarify the roles of eosinophils in ICD and the underlying mechanism of eosinophil infiltration of the skin. Methods We induced croton oil-induced ICD in eosinophil-deficient ΔdblGATA mice with or without a reactive oxygen species (ROS) inhibitor. We performed cocultivation with fibroblasts and bone marrow-derived basophils and evaluated eosinophil migration using a chemotaxis assay. Results ICD responses were significantly attenuated in the absence of eosinophils or by treatment with the ROS inhibitor. ROS was produced abundantly by eosinophils, and both basophils and eosinophils were detected in human and murine ICD skin lesions. In coculture experiments, basophils attracted eosinophils, especially in the presence of fibroblasts. Moreover, basophils produced IL-4 and TNF-α in contact with fibroblasts and promoted the expression of eotaxin/CCL11 from fibroblasts in vitro. Conclusion Eosinophils mediated the development of murine ICD, possibly through ROS production. Recruitment of eosinophils into the skin was induced by basophils in cooperation with fibroblasts. Our findings introduce the novel concept that basophils promote the recruitment of eosinophils into the skin through fibroblasts in the development of skin inflammation.",
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AU - Egawa, Gyohei

AU - Nakajima, Saeko

AU - Nakamizo, Satoshi

AU - Arita, Makoto

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AU - Miyachi, Yoshiki

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AB - Background Although eosinophils have been detected in several human skin diseases in the vicinity of basophils, how eosinophils infiltrate the skin and the role of eosinophils in the development of skin inflammation have yet to be examined. Objective Using murine irritant contact dermatitis (ICD) as a model, we sought to clarify the roles of eosinophils in ICD and the underlying mechanism of eosinophil infiltration of the skin. Methods We induced croton oil-induced ICD in eosinophil-deficient ΔdblGATA mice with or without a reactive oxygen species (ROS) inhibitor. We performed cocultivation with fibroblasts and bone marrow-derived basophils and evaluated eosinophil migration using a chemotaxis assay. Results ICD responses were significantly attenuated in the absence of eosinophils or by treatment with the ROS inhibitor. ROS was produced abundantly by eosinophils, and both basophils and eosinophils were detected in human and murine ICD skin lesions. In coculture experiments, basophils attracted eosinophils, especially in the presence of fibroblasts. Moreover, basophils produced IL-4 and TNF-α in contact with fibroblasts and promoted the expression of eotaxin/CCL11 from fibroblasts in vitro. Conclusion Eosinophils mediated the development of murine ICD, possibly through ROS production. Recruitment of eosinophils into the skin was induced by basophils in cooperation with fibroblasts. Our findings introduce the novel concept that basophils promote the recruitment of eosinophils into the skin through fibroblasts in the development of skin inflammation.

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