Bay K 8644, a voltage-sensitive calcium channel agonist, facilitates secretion of atrial natriuretic polypeptide from isolated perfused rat hearts

Yoshihiko Saito, Kazuwa Nakao, Narito Morri, Akira Sugawara, Shozo Shiono, Takayuki Yamada, Hiroshi Itoh, Makoto Sakamito, Kazuyoshi Kurahashi, Motohatsu Fujiwara, Hiroo Imura

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Effects of Bay K 8644, a voltage-sensitive calcium channel agonist, on atrial natriuretic polypeptide (ANP) secretion from isolated rat hearts perfused with Krebs-Henseleit solution were investigated. After a ninetymin period for stabilization, coronary sinus effluents were collected every two min and ANP levels were measured by radioimmunoassay. The basal secretory rate of ANP was 1.65 ± 0.15 ng/min (mean ± standard error). Bay K 8644 stimulated ANP secretion dose-dependently. This stimulatory action was blocked by simultaneous administration of nifedipine, its competitive antagonist. Heart rate was also increased by Bay K 8644 administration. In the gel filtration study, the major secretory form of ANP corresponded to α-rat ANP, a 28-amino acid peptide. These results suggest that voltage-sensitive calcium channels are involved in two principal biological properties, contraction and ANP secretion, of atrial cardiocytes.

Original languageEnglish
Pages (from-to)1170-1176
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number3
DOIs
Publication statusPublished - 1986 Aug 14

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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