Bay K 8644, a voltage-sensitive calcium channel agonist, facilitates secretion of atrial natriuretic polypeptide from isolated perfused rat hearts

Yoshihiko Saito, Kazuwa Nakao, Narito Morri, Akira Sugawara, Shozo Shiono, Takayuki Yamada, Hiroshi Itoh, Makoto Sakamito, Kazuyoshi Kurahashi, Motohatsu Fujiwara, Hiroo Imura

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Effects of Bay K 8644, a voltage-sensitive calcium channel agonist, on atrial natriuretic polypeptide (ANP) secretion from isolated rat hearts perfused with Krebs-Henseleit solution were investigated. After a ninetymin period for stabilization, coronary sinus effluents were collected every two min and ANP levels were measured by radioimmunoassay. The basal secretory rate of ANP was 1.65 ± 0.15 ng/min (mean ± standard error). Bay K 8644 stimulated ANP secretion dose-dependently. This stimulatory action was blocked by simultaneous administration of nifedipine, its competitive antagonist. Heart rate was also increased by Bay K 8644 administration. In the gel filtration study, the major secretory form of ANP corresponded to α-rat ANP, a 28-amino acid peptide. These results suggest that voltage-sensitive calcium channels are involved in two principal biological properties, contraction and ANP secretion, of atrial cardiocytes.

Original languageEnglish
Pages (from-to)1170-1176
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number3
DOIs
Publication statusPublished - 1986 Aug 14
Externally publishedYes

Fingerprint

Calcium Channel Agonists
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
Rats
Peptides
Electric potential
Secretory Rate
Coronary Sinus
Calcium Channels
Nifedipine
Radioimmunoassay
Gel Chromatography
Effluents
Stabilization
Heart Rate
Gels
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Bay K 8644, a voltage-sensitive calcium channel agonist, facilitates secretion of atrial natriuretic polypeptide from isolated perfused rat hearts. / Saito, Yoshihiko; Nakao, Kazuwa; Morri, Narito; Sugawara, Akira; Shiono, Shozo; Yamada, Takayuki; Itoh, Hiroshi; Sakamito, Makoto; Kurahashi, Kazuyoshi; Fujiwara, Motohatsu; Imura, Hiroo.

In: Biochemical and Biophysical Research Communications, Vol. 138, No. 3, 14.08.1986, p. 1170-1176.

Research output: Contribution to journalArticle

Saito, Yoshihiko ; Nakao, Kazuwa ; Morri, Narito ; Sugawara, Akira ; Shiono, Shozo ; Yamada, Takayuki ; Itoh, Hiroshi ; Sakamito, Makoto ; Kurahashi, Kazuyoshi ; Fujiwara, Motohatsu ; Imura, Hiroo. / Bay K 8644, a voltage-sensitive calcium channel agonist, facilitates secretion of atrial natriuretic polypeptide from isolated perfused rat hearts. In: Biochemical and Biophysical Research Communications. 1986 ; Vol. 138, No. 3. pp. 1170-1176.
@article{2b9534ab216b4b8cba3e878f0fc92c2f,
title = "Bay K 8644, a voltage-sensitive calcium channel agonist, facilitates secretion of atrial natriuretic polypeptide from isolated perfused rat hearts",
abstract = "Effects of Bay K 8644, a voltage-sensitive calcium channel agonist, on atrial natriuretic polypeptide (ANP) secretion from isolated rat hearts perfused with Krebs-Henseleit solution were investigated. After a ninetymin period for stabilization, coronary sinus effluents were collected every two min and ANP levels were measured by radioimmunoassay. The basal secretory rate of ANP was 1.65 ± 0.15 ng/min (mean ± standard error). Bay K 8644 stimulated ANP secretion dose-dependently. This stimulatory action was blocked by simultaneous administration of nifedipine, its competitive antagonist. Heart rate was also increased by Bay K 8644 administration. In the gel filtration study, the major secretory form of ANP corresponded to α-rat ANP, a 28-amino acid peptide. These results suggest that voltage-sensitive calcium channels are involved in two principal biological properties, contraction and ANP secretion, of atrial cardiocytes.",
author = "Yoshihiko Saito and Kazuwa Nakao and Narito Morri and Akira Sugawara and Shozo Shiono and Takayuki Yamada and Hiroshi Itoh and Makoto Sakamito and Kazuyoshi Kurahashi and Motohatsu Fujiwara and Hiroo Imura",
year = "1986",
month = "8",
day = "14",
doi = "10.1016/S0006-291X(86)80405-3",
language = "English",
volume = "138",
pages = "1170--1176",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Bay K 8644, a voltage-sensitive calcium channel agonist, facilitates secretion of atrial natriuretic polypeptide from isolated perfused rat hearts

AU - Saito, Yoshihiko

AU - Nakao, Kazuwa

AU - Morri, Narito

AU - Sugawara, Akira

AU - Shiono, Shozo

AU - Yamada, Takayuki

AU - Itoh, Hiroshi

AU - Sakamito, Makoto

AU - Kurahashi, Kazuyoshi

AU - Fujiwara, Motohatsu

AU - Imura, Hiroo

PY - 1986/8/14

Y1 - 1986/8/14

N2 - Effects of Bay K 8644, a voltage-sensitive calcium channel agonist, on atrial natriuretic polypeptide (ANP) secretion from isolated rat hearts perfused with Krebs-Henseleit solution were investigated. After a ninetymin period for stabilization, coronary sinus effluents were collected every two min and ANP levels were measured by radioimmunoassay. The basal secretory rate of ANP was 1.65 ± 0.15 ng/min (mean ± standard error). Bay K 8644 stimulated ANP secretion dose-dependently. This stimulatory action was blocked by simultaneous administration of nifedipine, its competitive antagonist. Heart rate was also increased by Bay K 8644 administration. In the gel filtration study, the major secretory form of ANP corresponded to α-rat ANP, a 28-amino acid peptide. These results suggest that voltage-sensitive calcium channels are involved in two principal biological properties, contraction and ANP secretion, of atrial cardiocytes.

AB - Effects of Bay K 8644, a voltage-sensitive calcium channel agonist, on atrial natriuretic polypeptide (ANP) secretion from isolated rat hearts perfused with Krebs-Henseleit solution were investigated. After a ninetymin period for stabilization, coronary sinus effluents were collected every two min and ANP levels were measured by radioimmunoassay. The basal secretory rate of ANP was 1.65 ± 0.15 ng/min (mean ± standard error). Bay K 8644 stimulated ANP secretion dose-dependently. This stimulatory action was blocked by simultaneous administration of nifedipine, its competitive antagonist. Heart rate was also increased by Bay K 8644 administration. In the gel filtration study, the major secretory form of ANP corresponded to α-rat ANP, a 28-amino acid peptide. These results suggest that voltage-sensitive calcium channels are involved in two principal biological properties, contraction and ANP secretion, of atrial cardiocytes.

UR - http://www.scopus.com/inward/record.url?scp=0022551581&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022551581&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(86)80405-3

DO - 10.1016/S0006-291X(86)80405-3

M3 - Article

VL - 138

SP - 1170

EP - 1176

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -