BCG priming enhances endotoxin-induced acute lung injury independent of neutrophils

S. Tasaka, A. Ishizaka, T. Urano, K. Sayama, F. Sakamaki, H. Nakamura, T. Terashima, Y. Waki, Kenzo Soejima, Y. Oyamada, Seitaro Fujishima, M. Kanazawa

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Abstract

Bacillus Calmette Guerin (BCG) is known to increase susceptibility to endotoxin in some animal species. We investigated the effect of BCG-priming and the role of neutrophils in the priming process on the pathogenesis of acute lung injury caused by intravenously administered Escherichia call endotoxin (LPS). Guinea pigs were divided into seven groups: (1) control (n = 8), (2) BCG-alone (n = 6), (3) cyclophosphamide (CPA)-alone (n = 6), (4) CPA+LPS (n = 6), (5) LPS-alone (n = 6), (6) BCG+LPS (n = 6), and (7) BCG+CPA+LPS (n = 6). A BCG dose of 8 mg/kg was injected subcutaneously 10 d before the study. CPA was administered intraperitoneally to induce peripheral neutropenia. Animals were observed for 4 h after intravenous administration of 0.2 mg/kg of LPS. The plasma TNF level was measured 2 h after LPS challenge. Lung wet-to-dry weight ratio, [125I]albumin leakage in lung tissue, differential cell count in bronchoalveolar lavage (BAL) fluid, and histopathologic features were examined immediately after death. Although the LPS-alone group showed PMN accumulation in lung tissue, neither excess lung water nor increased albumin leakage was induced by this dose of LPS. The BCG+LPS group showed increased lung water, histopathologic edema, and increases in BAL fluid cell counts and plasma TNF in comparison with the LPS- alone group. The BCG+CPA+LPS group also showed enhanced lung injury comparable to that seen in the BCG+LPS group. In both the CPA-alone and the CPA+LPS groups, no parameter was increased as compared with those in the control group. We conclude that pretreatment with BCG enhances LPS-induced lung injury, possibly through the priming effect of mononuclear cells but independently of peripheral neutrophils.

Original languageEnglish
Pages (from-to)1041-1049
Number of pages9
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume152
Issue number3
Publication statusPublished - 1995

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Acute Lung Injury
Mycobacterium bovis
Endotoxins
Neutrophils
Cyclophosphamide
Lung
Bronchoalveolar Lavage Fluid
Lung Injury
Albumins
Cell Count
Escherichia
Control Groups
Water
Pulmonary Edema
Neutropenia
Intravenous Administration
Edema
Guinea Pigs
Weights and Measures

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Tasaka, S., Ishizaka, A., Urano, T., Sayama, K., Sakamaki, F., Nakamura, H., ... Kanazawa, M. (1995). BCG priming enhances endotoxin-induced acute lung injury independent of neutrophils. American Journal of Respiratory and Critical Care Medicine, 152(3), 1041-1049.

BCG priming enhances endotoxin-induced acute lung injury independent of neutrophils. / Tasaka, S.; Ishizaka, A.; Urano, T.; Sayama, K.; Sakamaki, F.; Nakamura, H.; Terashima, T.; Waki, Y.; Soejima, Kenzo; Oyamada, Y.; Fujishima, Seitaro; Kanazawa, M.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 152, No. 3, 1995, p. 1041-1049.

Research output: Contribution to journalArticle

Tasaka, S, Ishizaka, A, Urano, T, Sayama, K, Sakamaki, F, Nakamura, H, Terashima, T, Waki, Y, Soejima, K, Oyamada, Y, Fujishima, S & Kanazawa, M 1995, 'BCG priming enhances endotoxin-induced acute lung injury independent of neutrophils', American Journal of Respiratory and Critical Care Medicine, vol. 152, no. 3, pp. 1041-1049.
Tasaka S, Ishizaka A, Urano T, Sayama K, Sakamaki F, Nakamura H et al. BCG priming enhances endotoxin-induced acute lung injury independent of neutrophils. American Journal of Respiratory and Critical Care Medicine. 1995;152(3):1041-1049.
Tasaka, S. ; Ishizaka, A. ; Urano, T. ; Sayama, K. ; Sakamaki, F. ; Nakamura, H. ; Terashima, T. ; Waki, Y. ; Soejima, Kenzo ; Oyamada, Y. ; Fujishima, Seitaro ; Kanazawa, M. / BCG priming enhances endotoxin-induced acute lung injury independent of neutrophils. In: American Journal of Respiratory and Critical Care Medicine. 1995 ; Vol. 152, No. 3. pp. 1041-1049.
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AU - Ishizaka, A.

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AU - Nakamura, H.

AU - Terashima, T.

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AB - Bacillus Calmette Guerin (BCG) is known to increase susceptibility to endotoxin in some animal species. We investigated the effect of BCG-priming and the role of neutrophils in the priming process on the pathogenesis of acute lung injury caused by intravenously administered Escherichia call endotoxin (LPS). Guinea pigs were divided into seven groups: (1) control (n = 8), (2) BCG-alone (n = 6), (3) cyclophosphamide (CPA)-alone (n = 6), (4) CPA+LPS (n = 6), (5) LPS-alone (n = 6), (6) BCG+LPS (n = 6), and (7) BCG+CPA+LPS (n = 6). A BCG dose of 8 mg/kg was injected subcutaneously 10 d before the study. CPA was administered intraperitoneally to induce peripheral neutropenia. Animals were observed for 4 h after intravenous administration of 0.2 mg/kg of LPS. The plasma TNF level was measured 2 h after LPS challenge. Lung wet-to-dry weight ratio, [125I]albumin leakage in lung tissue, differential cell count in bronchoalveolar lavage (BAL) fluid, and histopathologic features were examined immediately after death. Although the LPS-alone group showed PMN accumulation in lung tissue, neither excess lung water nor increased albumin leakage was induced by this dose of LPS. The BCG+LPS group showed increased lung water, histopathologic edema, and increases in BAL fluid cell counts and plasma TNF in comparison with the LPS- alone group. The BCG+CPA+LPS group also showed enhanced lung injury comparable to that seen in the BCG+LPS group. In both the CPA-alone and the CPA+LPS groups, no parameter was increased as compared with those in the control group. We conclude that pretreatment with BCG enhances LPS-induced lung injury, possibly through the priming effect of mononuclear cells but independently of peripheral neutrophils.

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