Bcl-2 related proteins are dramatically induced at the early stage of differentiation in human liver cancer cells by a histone deacetylase inhibitor projecting an anti-apoptotic role during this period

K. Wakabayashi, Hidetsugu Saito, H. Ebinuma, Yoshimasa Saito, T. Takagi, M. Nakamura, A. Umezawa, J. Hata, H. Ishii

Research output: Contribution to journalArticle

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Abstract

Expression of the Bcl-2 family members in a human hepatocellular carcinoma cell line (HCC-T) after sodium butyrate-treatment was investigated. Sodium butyrate, a histone deacetylase inhibitor, induced differentiation of the cell line into its normal counterpart without inducing apoptosis at the concentration of 2 mmol/1. Since sodium butyrate has effects on both differentiation and apoptosis, we investigated the expression profile of bcl-2 related genes in HCC-T. The expression of Bcl-2 and Mcl-1/EAT was up-regulated 4-12 h after the treatment while Bcl-XLwas up-regulated 2-3 days after the stimulation. On the other hand, the expression levels of Bax protein remained unchanged during differentiation. The HCC-T cells entered a cell cycle arrest at G1and showed neither cellular fragmentation nor apoptosis during this period, which was concomitantly associated with up-regulated expression of a cell cycle regulator, p21WAF-1. These results demonstrate that induction of anti-apoptotic Bcl-2 related proteins at an early stage of differentiation is important for the maintenance of HCC-T cell differentiation by antagonizing pro-apoptotic molecules such as Bax.

Original languageEnglish
Pages (from-to)285-288
Number of pages4
JournalOncology Reports
Volume7
Issue number2
Publication statusPublished - 2000

Fingerprint

Histone Deacetylase Inhibitors
Butyric Acid
Liver Neoplasms
Apoptosis
T-Lymphocytes
bcl-2 Genes
bcl-2-Associated X Protein
Cell Line
Proteins
Cell Cycle Checkpoints
Cell Differentiation
Hepatocellular Carcinoma
Cell Cycle
Maintenance

Keywords

  • Apoptosis
  • Bcl-2 related proteins
  • Differentiation
  • Hepatocellular carcinoma
  • Histone deacetylase inhibitor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Bcl-2 related proteins are dramatically induced at the early stage of differentiation in human liver cancer cells by a histone deacetylase inhibitor projecting an anti-apoptotic role during this period. / Wakabayashi, K.; Saito, Hidetsugu; Ebinuma, H.; Saito, Yoshimasa; Takagi, T.; Nakamura, M.; Umezawa, A.; Hata, J.; Ishii, H.

In: Oncology Reports, Vol. 7, No. 2, 2000, p. 285-288.

Research output: Contribution to journalArticle

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AU - Saito, Hidetsugu

AU - Ebinuma, H.

AU - Saito, Yoshimasa

AU - Takagi, T.

AU - Nakamura, M.

AU - Umezawa, A.

AU - Hata, J.

AU - Ishii, H.

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